scholarly journals A synthetic medium for the selection of yeasts able to utilize fatty acids as the sole carbon source.

1996 ◽  
Vol 42 (1) ◽  
pp. 87-91 ◽  
Author(s):  
ESTEBAN C. DELL'ANGELICA ◽  
DANIEL MILIKOWSKI ◽  
DANIEL A. SAENZ ◽  
CARLOS A. STELLA ◽  
EUGENIA H. RAMOS ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Selection Of

Rhamnolipids stabilize quorum sensing mediated cooperation in Pseudomonas aeruginosa

2020 ◽  
Vol 367 (10) ◽  
Author(s):  
Rodolfo García-Contreras ◽  
Daniel Loarca ◽  
Caleb Pérez-González ◽  
J Guillermo Jiménez-Cortés ◽  
Abigail Gonzalez-Valdez ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Carbon Source ◽  
Public Goods ◽  
Sole Carbon Source ◽  
Regulatory Genes ◽  
Chronic Infections ◽  
Toxic Metabolites ◽  
Serial Cultivation ◽  
Selection Of

ABSTRACT Pseudomonas aeruginosa is one of the main models to study social behaviors in bacteria since it synthesizes several exoproducts, including exoproteases and siderophores and release them to the environment. Exoproteases and siderophores are public goods that can be utilized by the individuals that produce them but also by non-producers, that are considered social cheaters. Molecularly exoprotease cheaters are mutants in regulatory genes such as lasR, and are commonly isolated from chronic infections and selected in the laboratory upon serial cultivation in media with protein as a sole carbon source. Despite that the production of exoproteases is exploitable, cooperators have also ways to restrict the growth and selection of social cheaters, for instance by producing toxic metabolites like pyocyanin. In this work, using bacterial competitions, serial cultivation and growth assays, we demonstrated that rhamnolipids which production is regulated by quorum sensing, selectively affect the growth of lasR mutants and are able to restrict social cheating, hence contributing to the maintenance of cooperation in Pseudomonas aeruginosa populations.

scholarly journals Effect of Lactose on Soluble-glucan Production and on the Ultrastructure of Sclerotium rolfsii Sacc. Grown in Submerged Culture

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Y. Okon ◽  
I. Chet ◽  
Naomi Kislev ◽  
Y. Henis
Keyword(s):  
Carbon Source ◽  
Submerged Culture ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Sclerotium Rolfsii ◽  
Dry Weight ◽  
Solid Media

SUMMARY A significant increase in mycelial dry weight and a decrease in production of extracellular glucan were observed when lactose was added to a submerged culture of Sclerotium rolfsii grown in a liquid glucose-synthetic medium. When added as a sole carbon source, lactose at 2·5% (w/v) induced the formation of dark, spherical, compact bodies. The ultrastructure of these bodies is compared with that of sclerotia formed on solid media.

The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production

2011 ◽  
Vol 102 (3) ◽  
pp. 2695-2701 ◽  
Author(s):  
Qiang Fei ◽  
Ho Nam Chang ◽  
Longan Shang ◽  
Jin-dal-rae Choi ◽  
NagJong Kim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Lipid Accumulation ◽  
Sole Carbon Source ◽  
Volatile Fatty Acids ◽  
Biodiesel Production ◽  
Cryptococcus Albidus

scholarly journals Genetically Modified Strains of Ralstonia eutropha H16 with β-Ketothiolase Gene Deletions for Production of Copolyesters with Defined 3-Hydroxyvaleric Acid Contents

2012 ◽  
Vol 78 (15) ◽  
pp. 5375-5383 ◽  
Author(s):  
Nicole Lindenkamp ◽  
Elena Volodina ◽  
Alexander Steinbüchel
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Ralstonia Eutropha ◽  
Carbon Sources ◽  
Production Capacity ◽  
Wild Type ◽  
Content Type ◽  
Storage Behavior ◽  
Copolymer Poly

ABSTRACTβ-Ketothiolases catalyze the first step of poly(3-hydroxybutyrate) [poly(3HB)] biosynthesis in bacteria by condensation of two acetyl coenzyme A (acetyl-CoA) molecules to acetoacetyl-CoA and also take part in the degradation of fatty acids. During growth on propionate or valerate,Ralstonia eutrophaH16 produces the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)]. InR. eutropha, 15 β-ketothiolase homologues exist. The synthesis of 3-hydroxybutyryl-CoA (3HB-CoA) could be significantly reduced in an 8-fold mutant (Lindenkamp et al., Appl. Environ. Microbiol. 76:5373–5382, 2010). In this study, a 9-fold mutant deficient in nine β-ketothiolase gene homologues (phaA,bktB, H16_A1713, H16_B1771, H16_A1528, H16_B0381, H16_B1369, H16_A0170, andpcaF) was generated. In order to examine the polyhydroxyalkanoate production capacity when short- or long-chain and even- or odd-chain-length fatty acids were provided as carbon sources, the growth and storage behavior of several mutants from the previous study and the newly generated 9-fold mutant were analyzed. Propionate, valerate, octanoate, undecanoic acid, or oleate was chosen as the sole carbon source. On octanoate, no significant differences in growth or storage behavior were observed between wild-typeR. eutrophaand the mutants. In contrast, during the growth on oleate of a multiple mutant lackingphaA,bktB, and H16_A0170, diminished poly(3HB) accumulation occurred. Surprisingly, the amount of accumulated poly(3HB) in the multiple mutants grown on gluconate differed; it was much lower than that on oleate. The β-ketothiolase activity toward acetoacetyl-CoA in H16ΔphaAand all the multiple mutants remained 10-fold lower than the activity of the wild type, regardless of which carbon source, oleate or gluconate, was employed. During growth on valerate as a sole carbon source, the 9-fold mutant accumulated almost a poly(3-hydroxyvalerate) [poly(3HV)] homopolyester with 99 mol% 3HV constituents.

scholarly journals ESTIMATION OF ANTIBACTERIAL EFFECT ON VIBRIO CHOLERAE (ON THE EXAMPLE OF THE SUBSTANCE ACETYL-N- CYSTEINE–L)

2019 ◽  
pp. 55-57
Author(s):  
O.V. Duvanova ◽  
B.N. Mishan′kin
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Carbon Source ◽  
Vibrio Cholerae ◽  
Sole Carbon Source ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Synthetic Medium ◽  
Nutrient Media ◽  
Cholera Vibrio ◽  
El Tor

We studied the effect of the substance acetyl-N-cysteine-L on cholera Vibrio. Its minimum inhibitory concentration for the strains of Vibrio cholerae El Tor O1 and O139 serogroup was 1–2.5 mg/ml in solid nutrient media - Martin's agar (pH 7.6) and LB (pH 7.2), while in Bhascaran synthetic medium, using glucose (0.1per cent) as the sole carbon source, N-acetyl-β-D-glucosamine (0.05 per cent) or colloidal chitin (0.027 per cent) it was reduced to 50–250 μg/ml. The ability of the substance acetyl-N-cysteine-L substance to suppress the activity of the purified enzyme N-acetyl-β-D-glucosaminidase (chitobiasis) was found. Antibacterial effect detected of the substance acetyl-N-cysteine-L against the strains of Vibrio cholerae El Tor O1 and O139 serogroups with different epidemic significance (presence / absence of ctxAB and tcpA genes) indicates the advisability of considering the issue on the possibility of including this substance in composition of solution components used in the rehydration therapy of diarrheal diseases cases.

GROWTH OF FUSARIUM DIVERSISPORUM SHERB. ON LONG-CHAINn-FATTY ALCOHOLS OR CHOLESTEROL AS THE SOLE CARBON SOURCE

1967 ◽  
Vol 13 (2) ◽  
pp. 121-136 ◽  
Author(s):  
Rudolf G. Strobel ◽  
Herbert Quinn ◽  
Willy Lange
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Unsaturated Fatty Acids ◽  
Submerged Cultures ◽  
Reserve Material ◽  
Lipid Contents ◽  
Cell Components ◽  
Metabolic Action

In well-aerated submerged cultures of Fusarium diversisporum Sherb. with n-hexadecanol or n-heptadecanol (which are virtually water insoluble) as the sole carbon source, the alkanols do not undergo extracellular chemical changes before assimilation but move, unchanged, through the cell wall faster than they can be metabolized by the organism and thus may constitute up to one-half of the total lipids in the cells. The alkanols are initially oxidized at the hydroxylated terminal carbon atom to fatty acids without loss of carbon. The fatty acids are subject to further metabolic action. Apparently carbon, not needed immediately for energy and for synthesis of cell components, is transformed into triglycerides as a reserve material. In these triglycerides, the distribution of the saturated and the unsaturated fatty acids between the primary and the secondary positions of the glycerol moiety is one typical of a vegetable lipid. Interesting differences exist, particularly in the sterol ester and phospho- or glyco-lipid contents, between mycelia grown on sucrose, hexadecanol, or heptadecanol. These cell constituents may possibly be involved in alkanol transport across the cell wall. The mold also assimilates cholesterol but has difficulty in metabolizing it.

Effects of cysteine and structurally related compounds on ochratoxin production by Aspergillus ochraceus

1985 ◽  
Vol 31 (11) ◽  
pp. 973-976 ◽  
Author(s):  
N. Lisker ◽  
N. Paster ◽  
I. Chet
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Fungal Growth ◽  
Toxin Production ◽  
Aspergillus Ochraceus ◽  
Homocysteine Thiolactone ◽  
The Media ◽  
Related Compounds

Addition of 10−2 ML-cysteine, L-cystine, or S-ethyl-L-cysteine to a synthetic medium containing xylose as the sole carbon source did not decrease ochratoxin production by Aspergillus ochraceus. At that concentration, DL-homocysteine thiolactone HCl, DL-cysteine HCl, L-ethionine, S-methyl-L-cysteine, and glutathione (reduced) strongly inhibited ochratoxin production. DL-Homocysteine thiolactone HCl, DL-cysteine HCl, and L-ethionine also strongly inhibited fungal growth. At lower concentrations (10−3and 10−4 M) only L-ethionine decreased the toxin production. Ochratoxin inhibition caused by DL-homocysteine thiolactone HCl, DL-cysteine HCl, and glutathione was observed only in cases where the pH of the media was below 5.0. The inhibition caused by 10−3 M ethionine was partially prevented by the addition of 10−3 M methionine but this was not the case after the addition of S-methyl-L-cysteine to the medium.

scholarly journals Role of Sinorhizobium meliloti and Escherichia coli Long-Chain Acyl-CoA Synthetase FadD in Long-Term Survival

2020 ◽  
Vol 8 (4) ◽  
pp. 470
Author(s):  
Ángel de la Cruz Pech-Canul ◽  
Geovanny Rivera-Hernández ◽  
Joaquina Nogales ◽  
Otto Geiger ◽  
María J. Soto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Carbon Source ◽  
Stationary Phase ◽  
Sole Carbon Source ◽  
Sinorhizobium Meliloti ◽  
Membrane Lipids ◽  
Term Survival ◽  
Long Chain

FadD is an acyl-coenzyme A (CoA) synthetase specific for long-chain fatty acids (LCFA). Strains mutated in fadD cannot produce acyl-CoA and thus cannot grow on exogenous LCFA as the sole carbon source. Mutants in the fadD (smc02162) of Sinorhizobium meliloti are unable to grow on oleate as the sole carbon source and present an increased surface motility and accumulation of free fatty acids at the entry of the stationary phase of growth. In this study, we found that constitutive expression of the closest FadD homologues of S. meliloti, encoded by sma0150 and smb20650, could not revert any of the mutant phenotypes. In contrast, the expression of Escherichia coli fadD could restore the same functions as S. meliloti fadD. Previously, we demonstrated that FadD is required for the degradation of endogenous fatty acids released from membrane lipids. Here, we show that absence of a functional fadD provokes a significant loss of viability in cultures of E. coli and of S. meliloti in the stationary phase, demonstrating a crucial role of fatty acid degradation in survival capacity.

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