Effects of cysteine and structurally related compounds on ochratoxin production by Aspergillus ochraceus

1985 ◽  
Vol 31 (11) ◽  
pp. 973-976 ◽  
Author(s):  
N. Lisker ◽  
N. Paster ◽  
I. Chet
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Fungal Growth ◽  
Toxin Production ◽  
Aspergillus Ochraceus ◽  
Homocysteine Thiolactone ◽  
The Media ◽  
Related Compounds

Addition of 10−2 ML-cysteine, L-cystine, or S-ethyl-L-cysteine to a synthetic medium containing xylose as the sole carbon source did not decrease ochratoxin production by Aspergillus ochraceus. At that concentration, DL-homocysteine thiolactone HCl, DL-cysteine HCl, L-ethionine, S-methyl-L-cysteine, and glutathione (reduced) strongly inhibited ochratoxin production. DL-Homocysteine thiolactone HCl, DL-cysteine HCl, and L-ethionine also strongly inhibited fungal growth. At lower concentrations (10−3and 10−4 M) only L-ethionine decreased the toxin production. Ochratoxin inhibition caused by DL-homocysteine thiolactone HCl, DL-cysteine HCl, and glutathione was observed only in cases where the pH of the media was below 5.0. The inhibition caused by 10−3 M ethionine was partially prevented by the addition of 10−3 M methionine but this was not the case after the addition of S-methyl-L-cysteine to the medium.

Ochratoxin production by Aspergillus ochraceus as affected by methionine and structurally related compounds

1983 ◽  
Vol 29 (5) ◽  
pp. 536-540 ◽  
Author(s):  
N. Lisker ◽  
N. Paster ◽  
I. Chet
Keyword(s):  
Radial Growth ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Methionine Sulfoxide ◽  
Dry Weight ◽  
Aspergillus Ochraceus ◽  
Inhibitory Effects ◽  
Methionine Sulfone ◽  
Initial Ph ◽  
Related Compounds

When 10−2 M of L- or D-methionine was added to a synthetic medium containing xylose as the sole carbon source, ochratoxin production by Aspergillus ochraceus was strongly inhibited. At that concentration methionine derivatives, e.g., α-methyl-DL-methionine, DL-methionine sulfoxide, and L-methionine sulfone, did not inhibit ochratoxin production, whereas DL-methionine S-methyl sulfonium chloride (MMSC) inhibited ochratoxin production to a large extent. L-Methionine, as well as MMSC, also completely inhibited sclerotia formation, while D-methionine and DL-methionine sulfoxide caused only a partial inhibition. At lower concentrations (10−3 and 10−4 M), none of the compounds exhibited inhibitory effects. In cases where strong ochratoxin inhibition was detected, fungal radial growth or mycelial dry weight was inhibited by only 10–25%, while the initial pH of the medium dropped from ~6.5 to ~4.4–5.0. Adjustment of the initial pH of media supplemented with 10−2 ML-methionine, D-methionine, or MMSC to a pH of ~7.8 did not change the inhibitory effects on ochratoxin production in media containing L-methionine. On the other hand, sclerotia formation was restored in all three treatments.

scholarly journals Effect of Lactose on Soluble-glucan Production and on the Ultrastructure of Sclerotium rolfsii Sacc. Grown in Submerged Culture

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Y. Okon ◽  
I. Chet ◽  
Naomi Kislev ◽  
Y. Henis
Keyword(s):  
Carbon Source ◽  
Submerged Culture ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Sclerotium Rolfsii ◽  
Dry Weight ◽  
Solid Media

SUMMARY A significant increase in mycelial dry weight and a decrease in production of extracellular glucan were observed when lactose was added to a submerged culture of Sclerotium rolfsii grown in a liquid glucose-synthetic medium. When added as a sole carbon source, lactose at 2·5% (w/v) induced the formation of dark, spherical, compact bodies. The ultrastructure of these bodies is compared with that of sclerotia formed on solid media.

scholarly journals A synthetic medium for the selection of yeasts able to utilize fatty acids as the sole carbon source.

1996 ◽  
Vol 42 (1) ◽  
pp. 87-91 ◽  
Author(s):  
ESTEBAN C. DELL'ANGELICA ◽  
DANIEL MILIKOWSKI ◽  
DANIEL A. SAENZ ◽  
CARLOS A. STELLA ◽  
EUGENIA H. RAMOS ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Selection Of

scholarly journals Characterization of Butachlor Degradation by A Molybdenum-Reducing and Aniline-degrading Pseudomonas sp.

2021 ◽  
Vol 9 (2) ◽  
pp. 8-12
Author(s):  
A.J. Sufyan ◽  
S. Ibrahim ◽  
A. Babandi ◽  
Hafeez Muhammad Yakasai
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Maximum Growth ◽  
Culture Collection ◽  
Inoculum Size ◽  
Lag Phase ◽  
Pseudomonas Sp ◽  
Characterization Study ◽  
The Media ◽  
Dna Strand

Butachlor is a chloroacetanilide herbicide that is selective in action and commonly used for pre-emergence control of weeds. It is believed to have range of toxicity from acute to chronic and also can cause DNA strand breaks and chromosomal aberrations in humans. This study was aimed at characterizing the potential of previously isolated bacteria for butachlor degradation. The isolates from culture collection, labelled A-F were screened for butachlor degradation on Bushnell Hass agar media with butachlor as a sole carbon source. Isolate A (molybdenum-reducing and aniline-degrading Pseudomonas sp.) was observed to grow best and tolerated the highest concentration of butachlor supplemented in the media after 72 h of incubation at 37 ℃. Characterization study revealed that the Pseudomonas sp. can utilize and grow with butachlor at optimum pH between 6.0 - 6.5, temperature between 30 – 37 ℃ and can tolerate up to 600 mg/L butachlor concentration with increase in growth as inoculum size increases. Additionally, this bacterial strain shows no lag phase regardless of the concentration of the herbicide used and reach its maximum growth after 24 h of incubation. The ability of this isolate to tolerate and utilize butachlor as sole carbon source makes it suitable for future bioremediation of this toxicant.

scholarly journals ESTIMATION OF ANTIBACTERIAL EFFECT ON VIBRIO CHOLERAE (ON THE EXAMPLE OF THE SUBSTANCE ACETYL-N- CYSTEINE–L)

2019 ◽  
pp. 55-57
Author(s):  
O.V. Duvanova ◽  
B.N. Mishan′kin
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Carbon Source ◽  
Vibrio Cholerae ◽  
Sole Carbon Source ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Synthetic Medium ◽  
Nutrient Media ◽  
Cholera Vibrio ◽  
El Tor

We studied the effect of the substance acetyl-N-cysteine-L on cholera Vibrio. Its minimum inhibitory concentration for the strains of Vibrio cholerae El Tor O1 and O139 serogroup was 1–2.5 mg/ml in solid nutrient media - Martin's agar (pH 7.6) and LB (pH 7.2), while in Bhascaran synthetic medium, using glucose (0.1per cent) as the sole carbon source, N-acetyl-β-D-glucosamine (0.05 per cent) or colloidal chitin (0.027 per cent) it was reduced to 50–250 μg/ml. The ability of the substance acetyl-N-cysteine-L substance to suppress the activity of the purified enzyme N-acetyl-β-D-glucosaminidase (chitobiasis) was found. Antibacterial effect detected of the substance acetyl-N-cysteine-L against the strains of Vibrio cholerae El Tor O1 and O139 serogroups with different epidemic significance (presence / absence of ctxAB and tcpA genes) indicates the advisability of considering the issue on the possibility of including this substance in composition of solution components used in the rehydration therapy of diarrheal diseases cases.

Growth and Production of Mycotoxins by Alternaria alternata in Synthetic, Semisynthetic and Rice Media

1985 ◽  
Vol 48 (4) ◽  
pp. 306-311 ◽  
Author(s):  
CHENG–I WEI ◽  
DIANE D. SWARTZ
Keyword(s):  
Carbon Source ◽  
Alternaria Alternata ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Toxin Production ◽  
Liquid Media ◽  
Rice Culture ◽  
Glucose Levels ◽  
Alternariol Methyl Ether ◽  
Cultivation Period

Alternaria alternata RL671-2 was cultivated in synthetic, semisynthetic or a rice culture medium to study its growth, and production of the mycotoxins alternariol (AOH), alternariol methyl ether (AME), and alteneune (ALT). Toxins were produced during the later stage of growth in both liquid media. Greater toxin production was found in semisynthetic (560 μg/100 ml of medium) than in synthetic medium (135 μg/100 ml). The pH decreased from 4.0 to 2.1 during the cultivation period in the synthetic medium, while it increased from 5.1 to 6.8 in the semisynthetic medium. Reduction of carbon source (glucose) levels in the synthetic medium to change the C/N ratios from 12:1 to 6:1 or 3:1, greatly increased both production of toxins and fungal mycelial weights. At a C/N ratio of 6:1, the fungus produced toxins at a level close to that produced in the semisynthetic medium. The rice culture was more efficient for production of large quantities of toxin.

Improved procedure and colorimetric test for the detection of ortho- and meta-cleavage of protocatechuate by Pseudomonas isolates

1974 ◽  
Vol 20 (7) ◽  
pp. 1059-1061 ◽  
Author(s):  
J. C. G. Ottow ◽  
W. Zolg
Keyword(s):  
Carbon Source ◽  
Yeast Extract ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Clear Cut ◽  
Colorimetric Test ◽  
Ring Fission ◽  
Cut Method

A sensitive and clear-cut method for the demonstration of ortho- and meta-cleavage of protocatechuate is described. Pseudomonas strains to be tested for the mode of ring fission should be grown on a synthetic medium containing p-hydroxybenzoate, quinate, or glucose–yeast extract as sole carbon source. Suspensions of these organisms are tested for ring-fission mechanism to detect the constitutive or inducible nature of protocatechuate 3,4-oxygenase.

scholarly journals Isolation of Rhodocyclus gelatinosus from poultry slaughterhouse wastewater

2002 ◽  
Vol 45 (4) ◽  
pp. 445-449 ◽  
Author(s):  
Elisa Helena Giglio Ponsano ◽  
Pedro Magalhães Lacava ◽  
Marcos Franke Pinto
Keyword(s):  
Carbon Source ◽  
Photosynthetic Bacteria ◽  
Synthetic Medium ◽  
Poultry Feed ◽  
Gram Negative ◽  
Slaughterhouse Wastewater ◽  
Rhodocyclus Gelatinosus

Four cultures of photosynthetic bacteria isolated from poultry slaughterhouse wastewater were identified as Rhodocyclus gelatinosus based on the following properties: reddish color of cultures in synthetic medium, presence of motility, slightly curved Gram-negative rods morphology, gelatin liquefying activity, utilization of citrate as carbon source and production of bacteriochlorophyl a and carotenoids of the spirilloxanthin alternative series. R. gelatinosus may represent a source of nutrients and pigments with application in poultry feed.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp
Sign in / Sign up

Export Citation Format

Share Document