scholarly journals Efficient expression of Bacillus subtilis sporulation gene in Escherichia coli and purification of the gene product.

1985 ◽  
Vol 31 (1) ◽  
pp. 93-105 ◽  
Author(s):  
MASANAO ODA ◽  
RYOSAKU NOMI ◽  
YASUO KOBAYASHI
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Gene Product ◽  
Efficient Expression

A developmental gene product of Bacillus subtilis homologous to the sigma factor of Escherichia coli

Nature ◽  
1984 ◽  
Vol 312 (5992) ◽  
pp. 376-378 ◽  
Author(s):  
Patrick Stragier ◽  
Jean Bouvier ◽  
Céline Bonamy ◽  
Jekisiel Szulmajster
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Gene Product ◽  
Sigma Factor ◽  
Developmental Gene

scholarly journals Characterization of the Depletion of 2-C-Methyl-d-Erythritol-2,4-Cyclodiphosphate Synthase in Escherichia coli and Bacillus subtilis

2002 ◽  
Vol 184 (20) ◽  
pp. 5609-5618 ◽  
Author(s):  
Tracey L. Campbell ◽  
Eric D. Brown
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Bacillus Subtilis ◽  
Gene Product ◽  
Cell Walls ◽  
Isoprenoid Biosynthesis ◽  
Synthetic Lethal ◽  
E Coli

ABSTRACT The ispF gene product in Escherichia coli has been shown to catalyze the formation of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (MEC) in the deoxyxylulose (DOXP) pathway for isoprenoid biosynthesis. In this work, the E. coli gene ispF and its Bacillus subtilis orthologue, yacN, were deleted and conditionally complemented by expression of these genes from distant loci in the respective organisms. In E. coli, complementation was achieved through integration of ispF at the araBAD locus with control from the arabinose-inducible araBAD promoter, while in B. subtilis, yacN was placed at amyE under control of the xylose-inducible xylA promoter. In both cases, growth was severely retarded in the absence of inducer, consistent with these genes being essential for survival. E. coli cells depleted of MEC synthase revealed a filamentous phenotype. This was in contrast to the depletion of MEC synthase in B. subtilis, which resulted in a loss of rod shape, irregular septation, multicompartmentalized cells, and thickened cell walls. To probe the nature of the predominant deficiency of MEC synthase-depleted cells, we investigated the sensitivity of these conditionally complemented mutants, grown with various concentrations of inducer, to a wide variety antibiotics. Synthetic lethal behavior in MEC synthase-depleted cells was prevalent for cell wall-active antibiotics.

scholarly journals Glutamyl-tRNA reductase activity in Bacillus subtilis is dependent on the hemA gene product

1992 ◽  
Vol 281 (3) ◽  
pp. 843-850 ◽  
Author(s):  
I Schröder ◽  
L Hederstedt ◽  
C G Kannangara ◽  
S P Gough
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Molecular Mass ◽  
Gene Product ◽  
Reductase Activity ◽  
Gel Filtration ◽  
Wild Type ◽  
C5 Pathway ◽  
Hema Gene

The Bacillus subtilis hemAXCDBL operon encodes enzymes for the synthesis of 5-aminolaevuline acid via the C5 pathway (hemA and hemL) and uroporphyrinogen III (hemB, hemC and hemD). B. subtilis HemA protein (molecular mass 50 kDa) was overexpressed in hemA mutant of both Escherichia coli and B. subtilis. A mutant B. subtilis HemA protein with a Cys to Tyr change at position 105 was also overexpressed. Both wild-type and mutant HemA proteins migrated as oligomers (molecular mass greater than or equal to 230 kDa) on gel-filtration columns. All column fractions containing wild-type HemA protein had glutamyl-tRNA reductase activity. No glutamyl-tRNA reductase activity was found with the mutant HemA protein. It is concluded that the B. subtilis hemA gene product is identical to, or part of, the glutamyl-tRNA reductase of the C5 pathway.

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

Sign in / Sign up

Export Citation Format

Share Document