A developmental gene product of Bacillus subtilis homologous to the sigma factor of Escherichia coli

Nature ◽  
1984 ◽  
Vol 312 (5992) ◽  
pp. 376-378 ◽  
Author(s):  
Patrick Stragier ◽  
Jean Bouvier ◽  
Céline Bonamy ◽  
Jekisiel Szulmajster
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Gene Product ◽  
Sigma Factor ◽  
Developmental Gene

The Mycobacterium tuberculosis mysB gene product is a functional equivalent of the Escherichia coli sigma factor, KatF

Gene ◽  
1999 ◽  
Vol 240 (2) ◽  
pp. 361-370 ◽  
Author(s):  
Nicola J. Mulder ◽  
Ros E. Powles ◽  
Harold Zappe ◽  
Lafras M. Steyn
Keyword(s):  
Escherichia Coli ◽  
Mycobacterium Tuberculosis ◽  
Gene Product ◽  
Sigma Factor ◽  
Functional Equivalent

scholarly journals Complementation of a temperature sensitive Escherichia coli rpoD mutation using Lactobacillus sigma factors

2014 ◽  
Author(s):  
James Winkler ◽  
Katy Kao
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Sigma Factor ◽  
Lactobacillus Brevis ◽  
Sigma Factors ◽  
Model Organisms ◽  
Temperature Sensitive ◽  
Genomic Libraries ◽  
E Coli ◽  
Rna Polymerase Holoenzyme

Housekeeping sigma factors in the σ70 family, as components of the RNA polymerase holoenzyme, are responsible for regulating transcription of genes related to vegetative growth. While these factors are well understood in model organisms such asEscherichia coliandBacillus subtilis, little experimental work has focused on the sigma factors in members of theLactobacillusgenus such asLactobacillus brevisandLactobacillus plantarum. This study evaluates the ability of putative σ70 proteins fromL. brevis(σ70-Lb) andL. plantarum(σ70-Lp) to complement a temperature sensitive mutation in theE. coli285c σ70 protein. This report is the first to show that these heterologous sigma factors were capable of restoring the viability ofE. coli285c for growth at 40-43.5 °C, indicating the σ70-Lb and σ70-Lp are capable of initiating transcription in a complex with theE. coli285c RNA polymerase.These heterologous sigma factors may therefore be useful for improving biochemical knowledge of the sigma factor family or for use in the expression of hetereologous genomic libraries.

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