scholarly journals RpaB, an essential response regulator for high-light stress, is extensively involved in transcriptional regulation under light-intensity upshift conditions in Synechococcus elongatus PCC 7942

2020 ◽  
Vol 66 (2) ◽  
pp. 73-79
Author(s):  
Akira Yasuda ◽  
Daichi Inami ◽  
Mitsumasa Hanaoka
Keyword(s):  
Transcriptional Regulation ◽  
Light Intensity ◽  
Response Regulator ◽  
Light Stress ◽  
Synechococcus Elongatus ◽  
High Light ◽  
High Light Stress ◽  
Pcc 7942

scholarly journals Stable Reference Gene Selection for RT-qPCR Analysis inSynechococcus elongatusPCC 7942 under Abiotic Stresses

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Xiao Luo ◽  
Jiaxing Li ◽  
Tianliang Chang ◽  
Hongyan He ◽  
Yi Zhao ◽  
...  
Keyword(s):  
Reference Gene ◽  
Reference Genes ◽  
Time Course ◽  
Light Stress ◽  
Nacl Stress ◽  
High Light ◽  
Stress Conditions ◽  
Internal Reference ◽  
High Light Stress ◽  
Pcc 7942

Synechococcus elongatusPCC 7942 (S. elongatusPCC 7942) is a model cyanobacteria species for circadian clock mechanism studies. It has also been widely used as a bioreactor to produce biofuels and other metabolic products. Quantitative real-time PCR (qPCR) technology is the most commonly used method for studying the expression of specific genes, in which the relative expression level of target genes is calibrated by stably expressed internal reference genes. In this work, we examined the expression of nine candidate reference genes in time-course samples ofS. elongatusPCC 7942 under no treatment (control), NaCl-stress conditions, H2O2-stress conditions, and high light-stress conditions. Based on the qPCR amplification parameters, the stability ranking of these candidate reference genes was established by three statistical software programs, geNorm, NormFinder, and BestKeeper. Considering all the stress conditions or high light stress alone, the results showed that the combination ofprsandsecAwas the best choice for the double reference gene calibration method by qPCR. The combination ofsecAandppc,rimMandrnpA,rnpA,andilvDwas most stable under no treatment, NaCl-stress conditions, and H2O2-stress conditions, respectively.rimMwas stable under only special conditions and should be carefully chosen.16SandrnpBwere not suitable as internal reference genes forS. elongatusPCC 7942 qPCR experiments under all experimental conditions. To validate the above results, a cyanobacterial core clock gene,kaiC, was used to evaluate the actual performance of the optimized reference genes by qPCR, as well as the worst reference genes under different stress conditions. The results indicated that the best reference gene yielded more accurate calibration results for qPCR experiments carried out inS. elongatusPCC 7942 time-course samples.

Nitric oxide mediates abscisic acid induced light-tolerance in leaves of tall fescue under high-light stress

2013 ◽  
Vol 162 ◽  
pp. 1-10 ◽  
Author(s):  
Yuefei Xu ◽  
Juanjuan Fu ◽  
Xitong Chu ◽  
Yongfang Sun ◽  
He Zhou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Abscisic Acid ◽  
Tall Fescue ◽  
Light Stress ◽  
High Light ◽  
High Light Stress

Does Astaxanthin Protect Haematococcus against Light Damage?

1998 ◽  
Vol 53 (1-2) ◽  
pp. 93-100 ◽  
Author(s):  
Lu Fan ◽  
Avigad Vonshak ◽  
Aliza Zarka ◽  
Sammy Boussiba
Keyword(s):  
Light Intensity ◽  
Light Stress ◽  
Phosphate Starvation ◽  
High Light Intensity ◽  
High Irradiance ◽  
High Light ◽  
Protective Agent ◽  
Light Damage ◽  
Low Light ◽  
Very High

Abstract The photoprotective function of the ketocarotenoid astaxanthin in Haematococcus was questioned. When exposed to high irradiance and/or nutritional stress, green Haematococcus cells turned red due to accumulation of an immense quantity of the red pigment astaxanthin. Our results demonstrate that: 1) The addition of diphenylamine, an inhibitor of astaxanthin biosynthesis, causes cell death under high light intensity; 2) Red cells are susceptible to high light stress to the same extent or even higher then green ones upon exposure to a very high light intensity (4000 μmol photon m-2 s-1); 3) Addition of 1O2 generators (methylene blue, rose bengal) under noninductive conditions (low light of 100 (μmol photon m-2 s-1) induced astaxanthin accumulation. This can be reversed by an exogenous 1O2 quencher (histidine); 4) Histidine can prevent the accumulation of astaxanthin induced by phosphate starvation. We suggest that: 1) Astaxanthin is the result of the photoprotection process rather than the protective agent; 2) 1O2 is involved indirectly in astaxanthin accumulation process.

scholarly journals Glutathione and zeaxanthin formation during high light stress in foliose lichens

2008 ◽  
Vol 53 (No. 8) ◽  
pp. 340-344 ◽  
Author(s):  
J. Štepigová ◽  
H. Vráblíková ◽  
J. Lang ◽  
K. Večeřová ◽  
M. Barták
Keyword(s):  
Xanthophyll Cycle ◽  
Light Exposure ◽  
Light Stress ◽  
High Light ◽  
Short Term ◽  
Xanthophyll Cycle Pigments ◽  
High Light Stress ◽  
Fluorescence Label ◽  
Thiol Binding ◽  
Pure Acetone

In the presented study, we describe techniques for glutathione and pigment determination in lichens used in our laboratory. Glutathione and xanthophyll cycle pigments, especially zeaxanthin, are important antioxidants protecting plants against various stresses. In our laboratory, the high light stress in lichens has been intensively studied for several years. We extract glutathione in HCl and determine it by thiol-binding fluorescence label monobromobimane. For pigment determination, homogenized lichen thalli are extracted with pure acetone. According to our results, the total amount of glutathione decreases after a short-term high light exposure, while the amount of zeaxanthin increases.

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