scholarly journals Differences in the gingival crevicular fluid composition between adults and adolescents undergoing orthodontic treatment

2013 ◽  
Vol 84 (1) ◽  
pp. 120-126 ◽  
Author(s):  
Wellington J. Rody ◽  
Manjula Wijegunasinghe ◽  
William A. Wiltshire ◽  
Brenden Dufault
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Age Groups ◽  
Tissue Response ◽  
Fluid Composition ◽  
Age Trends ◽  
Crevicular Fluid ◽  
Changes Over Time ◽  
Microarray Technique

ABSTRACT Objectives: To investigate differences in the gingival crevicular fluid (GCF) composition between adolescent and adult patients undergoing orthodontic treatment with fixed appliances. Materials and Methods: Ten adolescents (14.4 ± 1.43) and 10 adults (28.5 ± 7.83) with Class I malocclusions and minor upper incisor crowding were allocated to two different age groups. Brackets were bonded only in the upper arch over the 20-week period of the experiment. Samples of GCF were collected from the labial sides of the upper incisors (experimental sites) and lower incisors (control sites) of each subject at five time points. Aliquots from diluted GCF were screened for the presence of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), interleukin-1 (IL-1), interleukin-1 receptor antagonist (IL-1RA), and metalloproteinase-9 (MMP-9) using a microarray technique. The values were statistically analyzed. Results: In adults, the ratio of IL-1 to IL-1RA decreased significantly (P  =  .033) in experimental sites 3 weeks after appliance placement and first archwire activation. In adolescents, the ratio of RANKL to OPG peaked 6 weeks after the insertion of the first rectangular archwire. This ratio peak found in adolescents was a consequence of a decrease in the mean concentration of OPG. No significant changes over time were observed in the concentration of MMP-9. Conclusion: This study demonstrates age trends in the GCF levels of IL-1, IL-1RA, RANKL, and OPG that may be used to track differences in tissue response between adults and adolescents undergoing orthodontic treatment.

scholarly journals Effects of Low-Level Diode Laser on the Level of Interleukin 1-Beta in the Gingival Crevicular Fluid and the Incidence of Gingivitis Caused by the Use of Orthodontic Separators: A Randomized Clinical Trial

2020 ◽  
Vol 12 (4) ◽  
pp. 120-125
Author(s):  
Sepideh Soheilifar ◽  
Sara Soheilifar ◽  
Shayesteh Hatami ◽  
Farshid Vahdatinia ◽  
Vahid Molabashi ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Laser Radiation ◽  
Diode Laser ◽  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Significant Difference ◽  
Orthodontic Separators ◽  
Crevicular Fluid

Background: In recent years, there has been an increased tendency for using dental lasers for the treatment of soft tissue problems. The aim of this study was to evaluate the effect of low-level diode laser (980 nm) on the level of interleukin 1 beta (IL-1β) in the gingival crevicular fluid (GCF) and the incidence of initial gingivitis caused by the use of orthodontic separators. Methods: In this randomized clinical trial, 30 patients, who were beginning a fixed orthodontic treatment without gingivitis, were randomly assigned to control and diode laser radiation (980 nm wavelength, 3 J of energy, a density of 3 J/cm2 , a power of 0.2 W, and at a distance of 1 cm away from the tissue for 15 seconds on the buccal and palatal sides of the tooth) groups. The gingival index (GI) and bleeding on probing (BOP) were measured at the beginning of the study and one week after the treatment. The level of IL-1β was evaluated using an enzyme-linked immunosorbent assay at the beginning of the study and one week after the placement of the separator. Finally, the inter-group and intra-group statistical analyses were performed using independent and paired t tests, and P<0.05 was considered as the significance level. Results: The evaluation of clinical variables in the entire mouth showed a slight clinical improvement in the experimental group although there was no significant difference between the two groups. No significant difference was observed between intra-group and inter-group evaluations of clinical indices in the studied specific teeth. Eventually, no difference was found between the two groups in terms of IL-1β changes. Conclusions: In general, the single-diode laser radiation session is not effective in the treatment of gingivitis in patients undergoing orthodontic treatment. Thus, it is recommended to perform frequent laser radiation sessions in further studies.

scholarly journals Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Simina Chelărescu ◽  
Petra Șurlin ◽  
Mioara Decusară ◽  
Mădălina Oprică ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Young Adults ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Early Stage ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Adolescents And Young Adults ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

Aspartate Aminotransferase Activity in Gingival Crevicular Fluid During Orthodontic Treatment. A Controlled Short-Term Longitudinal Study

2003 ◽  
Vol 74 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Giuseppe Perinetti ◽  
Michele Paolantonio ◽  
Michele D'Attilio ◽  
Domenico D'Archivio ◽  
Marco Dolci ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Aspartate Aminotransferase ◽  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Aminotransferase Activity ◽  
Short Term ◽  
Crevicular Fluid

scholarly journals Gingival Crevicular Fluid – Composition and Clinical Importance in Gingivitis and Periodontitis

2014 ◽  
Vol 124 (2) ◽  
Author(s):  
Mansur Rahnama ◽  
Łukasz Czupkałło ◽  
Maryla Kozicka-Czupkałło ◽  
Michał Łobacz
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Importance ◽  
Fluid Composition ◽  
Crevicular Fluid

scholarly journals Immunoassay analysis of proteins in gingival crevicular fluid samples from resorbing teeth

2015 ◽  
Vol 86 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Wellington J. Rody ◽  
Manjula Wijegunasinghe ◽  
L. Shannon Holliday ◽  
Kevin P. McHugh ◽  
Shannon M. Wallet
Keyword(s):  
Root Resorption ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Skewed Distribution ◽  
Mixed Dentition ◽  
Deciduous Teeth ◽  
Permanent Molar ◽  
Dentin Sialoprotein ◽  
Deciduous Molar ◽  
Crevicular Fluid

ABSTRACT Objective:  To carry out an immunoassay analysis of biomarkers expressed in gingival crevicular fluid (GCF) with the main goal of finding a useful diagnostic pattern to distinguish between resorbing deciduous teeth and nonresorbing controls. Materials and Methods:  A split-mouth design was used in this study with a total of 22 GCF samples collected from 11 patients in the mixed dentition. For each child, one deciduous molar with radiographic evidence of root resorption was used as the test tooth whereas the contralateral first permanent molar with formed roots was used as the control tooth. Samples were processed with immunoassays using a panel of selected biomarkers including interleukin-1 beta (IL-1b), interleukin-1 receptor antagonist (IL-1RA), nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase-9 (MMP-9), and dentin sialoprotein (DSP). Results:  There were no statistically significant differences in levels of IL-1b, OPG, and MMP-9 between test and control sites (P &gt; .05). IL-1RA was the only biomarker to show a significant down-regulation (P  =  .04) in GCF samples collected from resorbing teeth. RANKL data showed a heavily skewed distribution and was deemed unreliable. Only one deciduous GCF sample had detectable levels of DSP; therefore, no further statistical calculation was applicable because of the limited amount of data for this biomarker. Conclusions:  This study indicated that IL1-RA is down-regulated in GCF from resorbing primary molars, thus suggesting this cytokine as a potential analyte to be included in a panel that can discriminate between resorbing and nonresorbing teeth.

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