scholarly journals Multicellular Tumor Spheroids as an in Vivo–Like Tumor Model for Three-Dimensional Imaging of Chemotherapeutic and Nano Material Cellular Penetration

2012 ◽  
Vol 11 (6) ◽  
pp. 7290.2012.00012 ◽  
Author(s):  
Hui-li Ma ◽  
Qiao Jiang ◽  
Siyuan Han ◽  
Yan Wu ◽  
Jin Cui Tomshine ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Tumor Model ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Three Dimensional Imaging ◽  
Nano Material

scholarly journals Natural and Synthetic Biomaterials for Engineering Multicellular Tumor Spheroids

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2506
Author(s):  
Advika Kamatar ◽  
Gokhan Gunay ◽  
Handan Acar
Keyword(s):  
Cancer Research ◽  
Three Dimensional ◽  
3D Culture ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
In Vivo Models ◽  
Advantages And Disadvantages ◽  
Natural And Synthetic

The lack of in vitro models that represent the native tumor microenvironment is a significant challenge for cancer research. Two-dimensional (2D) monolayer culture has long been the standard for in vitro cell-based studies. However, differences between 2D culture and the in vivo environment have led to poor translation of cancer research from in vitro to in vivo models, slowing the progress of the field. Recent advances in three-dimensional (3D) culture have improved the ability of in vitro culture to replicate in vivo conditions. Although 3D cultures still cannot achieve the complexity of the in vivo environment, they can still better replicate the cell–cell and cell–matrix interactions of solid tumors. Multicellular tumor spheroids (MCTS) are three-dimensional (3D) clusters of cells with tumor-like features such as oxygen gradients and drug resistance, and represent an important translational tool for cancer research. Accordingly, natural and synthetic polymers, including collagen, hyaluronic acid, Matrigel®, polyethylene glycol (PEG), alginate and chitosan, have been used to form and study MCTS for improved clinical translatability. This review evaluates the current state of biomaterial-based MCTS formation, including advantages and disadvantages of the different biomaterials and their recent applications to the field of cancer research, with a focus on the past five years.

scholarly journals Impact of multicellular tumor spheroids as an in vivo-like tumor model on anticancer drug response

2016 ◽  
Vol 48 (6) ◽  
pp. 2295-2302 ◽  
Author(s):  
BIANCA GALATEANU ◽  
ARIANA HUDITA ◽  
CAROLINA NEGREI ◽  
RODICA-MARIANA ION ◽  
MARIETA COSTACHE ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Drug Response ◽  
Tumor Model ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Anticancer Drug Response

scholarly journals Challenges of applying multicellular tumor spheroids in preclinical phase

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Se Jik Han ◽  
Sangwoo Kwon ◽  
Kyung Sook Kim
Keyword(s):  
Solid Tumors ◽  
Therapeutic Efficacy ◽  
Three Dimensional ◽  
Biological Properties ◽  
Physical Interaction ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Efficacy Evaluation ◽  
Preclinical Phase

AbstractThe three-dimensional (3D) multicellular tumor spheroids (MCTs) model is becoming an essential tool in cancer research as it expresses an intermediate complexity between 2D monolayer models and in vivo solid tumors. MCTs closely resemble in vivo solid tumors in many aspects, such as the heterogeneous architecture, internal gradients of signaling factors, nutrients, and oxygenation. MCTs have growth kinetics similar to those of in vivo tumors, and the cells in spheroid mimic the physical interaction of the tumors, such as cell-to-cell and cell-to-extracellular matrix interactions. These similarities provide great potential for studying the biological properties of tumors and a promising platform for drug screening and therapeutic efficacy evaluation. However, MCTs are not well adopted as preclinical tools for studying tumor behavior and therapeutic efficacy up to now. In this review, we addressed the challenges with MCTs application and discussed various efforts to overcome the challenges.

scholarly journals Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy

2006 ◽  
Vol 11 (3) ◽  
pp. 034032 ◽  
Author(s):  
Jung-Taek Oh ◽  
Meng-Lin Li ◽  
Hao F. Zhang ◽  
Konstantin Maslov ◽  
George Stoica ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Dual Wavelength ◽  
Photoacoustic Microscopy ◽  
Skin Melanoma ◽  
Three Dimensional Imaging

Folic acid-conjugated soybean protein-based nanoparticles mediate efficient antitumor ability in vitro

2016 ◽  
Vol 31 (6) ◽  
pp. 832-843 ◽  
Author(s):  
Weijing Yao ◽  
Qian Zha ◽  
Xu Cheng ◽  
Xin Wang ◽  
Jun Wang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Soy Protein ◽  
Soybean Protein ◽  
Soy Protein Isolate ◽  
Three Dimensional ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Solution Ph ◽  
Protein Nanoparticles

In this study, soy protein isolate was hydrolyzed by compound enzymes to give aqueous soy protein with low molecular weights. Folic acid modified and free soy protein nanoparticles were successfully prepared by a desolvation method as target-specific drug delivery, respectively. Ultraviolet spectrophotometry demonstrated that folic acid was successfully grafted onto soy protein. The shape and size of folic acid modified soy protein nanoparticles were detected by transmission electron microscopy, scanning electron microscope, and dynamic light scattering. In addition, a series of characteristics including kinetic stability, pH stability, and time stability were also performed. Doxorubicin was successfully loaded into folic acid modified soy protein nanoparticles, and the encapsulation and loading efficiencies were 96.7% and 23%, respectively. Doxorubicin-loaded folic acid modified soy protein nanoparticles exhibited faster drug release rate than soy protein nanoparticles in PBS solution (pH = 5). The tumor penetration and antitumor experiments were done using three-dimensional multicellular tumor spheroids as the in vitro model. The results proved that folic acid modified soy protein nanoparticles display higher penetration and accumulation than soy protein nanoparticles, therefore possessing efficient growth inhibitory ability against multicellular tumor spheroids.

scholarly journals Micro-MRI at 11.7 T of a Murine Brain Tumor Model Using Delayed Contrast Enhancement

2003 ◽  
Vol 2 (3) ◽  
pp. 153535002003031
Author(s):  
Rex A. Moats ◽  
Sendhil Velan-Mullan ◽  
Russell Jacobs ◽  
Ignacio Gonzalez-Gomez ◽  
David J. Dubowitz ◽  
...  
Keyword(s):  
Brain Tumors ◽  
Tumor Growth ◽  
Intraperitoneal Administration ◽  
Three Dimensional ◽  
Tumor Model ◽  
Qualitative Assessment ◽  
Response To Treatment ◽  
Simple Method ◽  
Serial Measurement

In vivo imaging methodologies allow for serial measurement of tumor size, circumventing the need for sacrificing mice at given time points. In orthotopically transplanted murine models of brain tumors, cross-section micro-MRI allows for visualization and measurement of the physically inaccessible tumors. To allow for long resident times of a contrast agent in the tumor, intraperitoneal administration was used as a route of injection for contrast-enhanced micro-MRI, and a simple method for relative tumor volume measurements was examined. A strategy for visualizing the variability of the delayed tumor enhancement was developed. These strategies were applied to monitor the growth of brain tumors xenotransplanted into nude mice and either treated with the antiangiogenic peptide EMD 121974 or an inactive control peptide. Each mouse was used as its own control. Serial imaging was done weekly, beginning at Day 7 after tumor cell implantation and continued for 7 weeks. Images obtained were reconstructed on the MRI instrument. The image files were transferred off line to be postprocessed to assess tumor growth (volume) and variability in enhancement (three-dimensional [3-D] intensity models). In a small study, tumor growth and response to treatment were followed using this methodology and the high-resolution images displayed in 3-D allowed for straightforward qualitative assessment of variable enhancement related to vascular factors and tumor age.

scholarly journals In vivo, high-resolution, three-dimensional imaging of port wine stain microvasculature in human skin

2013 ◽  
Vol 45 (10) ◽  
pp. 628-632 ◽  
Author(s):  
Gangjun Liu ◽  
Wangcun Jia ◽  
J. Stuart Nelson ◽  
Zhongping Chen
Keyword(s):  
High Resolution ◽  
Human Skin ◽  
Three Dimensional ◽  
Port Wine Stain ◽  
Port Wine ◽  
Three Dimensional Imaging

Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

2013 ◽  
Vol 437 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Anika Nagelkerke ◽  
Johan Bussink ◽  
Fred C.G.J. Sweep ◽  
Paul N. Span
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Three Dimensional ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids
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