The Cultivation of Rickettsia diaporica in Tissue Culture and in the Tissues of Developing Chick Embryos

1939 ◽  
Vol 54 (49) ◽  
pp. 2171 ◽  
Author(s):  
Herald R. Cox ◽  
E. John Beli
Keyword(s):  
Tissue Culture ◽  
Chick Embryos

Cytotoxicity of lens antisera to dissociated chick neural retina cells in tissue culture

Development ◽  
1969 ◽  
Vol 21 (3) ◽  
pp. 391-406
Author(s):  
Max Braverman ◽  
Carl Cohen ◽  
Arthur Katoh
Keyword(s):  
Tissue Culture ◽  
Neural Retina ◽  
Vitreous Humour ◽  
Specific Antiserum ◽  
Chick Embryos ◽  
Ocular Tissues ◽  
Lens Proteins ◽  
Chick Lens

Immunoprecipitation techniques have shown that characteristic lens proteins can be found in many tissues of the chick eye. Langman & Prescott (1959), Maisel & Langman (1961a), Maisel (1962) and Maisel & Harmison (1963), among others, have demonstrated antigens cross-reacting with adult chick lens antisera in iris, pigmented retina, cornea and aqueous and vitreous humour. Maisel (1963) suggested that lens antigens are present in neural retina, but the presence of lens antigens in this tissue has not been firmly established, and a number of investigators reporting lens antigens in other ocular tissues have not found them in the neuro-retina. [For reviews of immunological investigations on the development and ubiquity of lens proteins see Langman (1959a, b), Maisel & Langman (1961b), Rabaey (1962), Woedereman (1961), Zwaan (1963), Ikeda & Zwaan (1966, 1967), Zwaan & Ikeda (1968) and Clayton, Campbell & Truman (1968).] Chick embryos developing in the presence of lens specific antiserum do, however, exhibit defects of the neural retina.

scholarly journals A STUDY OF TISSUE CULTURE CELLS BY ELECTRON MICROSCOPY

1945 ◽  
Vol 81 (3) ◽  
pp. 233-246 ◽  
Author(s):  
Keith R. Porter ◽  
Albert Claude ◽  
Ernest F. Fullam
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Electron Micrographs ◽  
Cell Structure ◽  
Culture Technique ◽  
Chick Embryos ◽  
Tissue Culture Technique ◽  
Culture Cells ◽  
Tissue Culture Cells

By means of a tissue culture technique, cells from chick embryos were procured in a state which proved to be suitable for electron microscopy. The electron micrographs disclosed details of cell structure not revealed by other methods of examination.

scholarly journals Identification of increased amounts of UDP-glucuronyltransferase protein in phenobarbital-treated chick-embryo liver cells

1983 ◽  
Vol 214 (2) ◽  
pp. 517-523 ◽  
Author(s):  
B Burchell ◽  
G J Pratt ◽  
I Duffy ◽  
L West
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Microsomal Fraction ◽  
Microsomal Protein ◽  
Liver Cells ◽  
Transferase Activity ◽  
Chick Embryos ◽  
Embryo Liver ◽  
Liver Microsomal Fraction ◽  
First Time

UDP-glucuronyltransferase activity of neonatal-chick liver or phenobarbital-treated chick-embryo liver catalysed the glucuronidation of 1-naphthol, 4-nitrophenol and 2-aminophenol. Only low transferase activity towards testosterone was detected, and activity towards bilirubin was not detectable. Liver microsomal transferase activity towards the three phenols was increased approx. 20-50-fold by phenobarbital treatment of chick embryos or by transfer of liver cells into tissue culture. A single form of UDP-glucuronyltransferase, which appears to catalyse the glucuronidation of these three phenols, was purified to near homogeneity from phenobarbital-treated chick-embryo liver microsomal fraction for the first time. The use of this purified enzyme as a standard protein facilitated the identification of this protein in chick-embryo liver microsomal fraction. Further, the accumulation of this microsomal protein was observed following phenobarbital treatment of chick embryos and during tissue culture of chick-embryo liver cells. The value of this model system for the study of the induction of UDP-glucuronyltransferase by drugs and hormones is discussed.

scholarly journals THE OCCURRENCE OF INTRACELLULAR CHONDROITIN SULFATE

1963 ◽  
Vol 18 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Frank K. Thorp ◽  
Albert Dorfman
Keyword(s):  
Tissue Culture ◽  
Infrared Spectroscopy ◽  
Chemical Analysis ◽  
Chondroitin Sulfate ◽  
Culture Medium ◽  
Extracellular Polysaccharide ◽  
Tissue Culture Medium ◽  
Extracellular Polysaccharides ◽  
Chick Embryos ◽  
The Matrix

Suspensions of chondrocytes were prepared by treatment with trypsin of the epiphyses of tibias and femurs of 13-day-old chick embryos. After washing to remove the matrix, such suspensions readily incorporate radioactive sulfate into both intracellular and extracellular chondroitin sulfate. Following disruption of the cells, the cell constituents were fractionated by centrifugation. Fractions obtained from cells incubated for 10 minutes showed a concentration of radioactivity in the material which sediments at 10,000 to 20,000 g. At this time the radioactivity of the extracellular chondroitin sulfate is low, but at 1 hour the radioactivity of the intracellular material is relatively unchanged, while that of the extracellular polysaccharide is markedly increased. Following incubation of the chondrocyte suspensions in a tissue culture medium, the intracellular chondroitin sulfate was isolated. This was compared with chondroitin sulfate isolated from the cartilage matrix. Chemical analysis and infrared spectroscopy indicated that both the intracellular and extracellular polysaccharides consist of a mixture of chondroitin sulfuric acids A and C. A portion of the chondroitin sulfate is not sulfated.

Studies on cardiac muscle cells, from chick embryos, grown in tissue culture

1938 ◽  
Vol 70 (4) ◽  
pp. 441-449 ◽  
Author(s):  
George S. de Rényi ◽  
Mary Jane Hogue
Keyword(s):  
Tissue Culture ◽  
Cardiac Muscle ◽  
Muscle Cells ◽  
Chick Embryos ◽  
Cardiac Muscle Cells
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