The Effects of X-Rays on the Growth of Spinal Ganglia from 6- and 12-Day Chick Embryos in Tissue Culture

1956 ◽  
Vol 5 (4) ◽  
pp. 390 ◽  
Author(s):  
Irene Plactere Goldring
Keyword(s):  
Tissue Culture ◽  
Spinal Ganglia ◽  
Chick Embryos ◽  
X Rays

scholarly journals STUDIES OF THE DEGENERATION AND REGENERATION OF AXIS CYLINDERS IN VITRO

1913 ◽  
Vol 17 (2) ◽  
pp. 182-191 ◽  
Author(s):  
Ragnvald Ingebrigtsen
Keyword(s):  
Spinal Cord ◽  
Degenerative Changes ◽  
Spinal Ganglia ◽  
Chick Embryos

1. The brains of chick embryos, of cats six weeks old, of rabbits two months old, and of dogs three weeks old, when cultivated in vitro, develop long filaments which, according to their growth and their anatomical and tinctorial characters, must be considered as true axis cylinders. 2. Similar structures develop from spinal ganglia of rabbits seven months old, and from the spinal cord of cats six weeks old, and of rabbits two months old. 3. When severed from their origin by section these threads undergo degenerative changes which do not appear after nine hours, but which are seen after twenty hours, and continue until in the course of the following two days the thread degenerates completely. 4. After twenty hours the development of new axis cylinders from the central part of the cut fibers is observed.

Cytotoxicity of lens antisera to dissociated chick neural retina cells in tissue culture

Development ◽  
1969 ◽  
Vol 21 (3) ◽  
pp. 391-406
Author(s):  
Max Braverman ◽  
Carl Cohen ◽  
Arthur Katoh
Keyword(s):  
Tissue Culture ◽  
Neural Retina ◽  
Vitreous Humour ◽  
Specific Antiserum ◽  
Chick Embryos ◽  
Ocular Tissues ◽  
Lens Proteins ◽  
Chick Lens

Immunoprecipitation techniques have shown that characteristic lens proteins can be found in many tissues of the chick eye. Langman & Prescott (1959), Maisel & Langman (1961a), Maisel (1962) and Maisel & Harmison (1963), among others, have demonstrated antigens cross-reacting with adult chick lens antisera in iris, pigmented retina, cornea and aqueous and vitreous humour. Maisel (1963) suggested that lens antigens are present in neural retina, but the presence of lens antigens in this tissue has not been firmly established, and a number of investigators reporting lens antigens in other ocular tissues have not found them in the neuro-retina. [For reviews of immunological investigations on the development and ubiquity of lens proteins see Langman (1959a, b), Maisel & Langman (1961b), Rabaey (1962), Woedereman (1961), Zwaan (1963), Ikeda & Zwaan (1966, 1967), Zwaan & Ikeda (1968) and Clayton, Campbell & Truman (1968).] Chick embryos developing in the presence of lens specific antiserum do, however, exhibit defects of the neural retina.

scholarly journals The immediate changes observed in tissue cells after exposure to soft X -rays while growing in vitro

1923 ◽  
Vol 95 (669) ◽  
pp. 373-381 ◽  
Keyword(s):  
Chick Embryos ◽  
X Rays ◽  
Tissue Cells

The experiments described in the following paper were carried out in order to study the immediate changes seen in tissue cells after exposure to soft X-rays while growing in vitro . The tissue from which the cultures were grown was obtained from the choroid of chick embryos of 6 to 7 days’ incubation.

scholarly journals The effect of gamma irradiation on cell division in tissue culture in vitro .—Part II

1929 ◽  
Vol 105 (734) ◽  
pp. 93-98 ◽  
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
X Rays ◽  
Tissue Cultures ◽  
Long Period ◽  
Silk Worm ◽  
Number Of Cells

In a previous paper (1) the effects of the gamma rays of radium upon the number of cells in mitosis in tissue cultures has been described, the cultures being examined 80 minutes after irradiation. Under the conditions of the experiment it was shown that there was a threshold of intensity below which no diminution in the number of cells in mitosis was apparent, and also a threshold of time for each intensity which must be exceeded before diminution could be observed. Gilman and Baetjer (2) showed that there was an acceleration in the development of the eggs of Amblystoma after irradiation by X-rays. Hastings, Beckton and Wedd (4) showed an increase in the rate of hatching out of silk-worm eggs which had been irradiated by X-rays; and Lazarus-Barlow and Beckton (3) showed that small intensities of beta-rays acting for a long period were followed by a greater rate of cell division in the eggs of Ascaris Megalocephala . In the case of tissue cultures Canti and Donaldson (5) described an experiment in which cessation of mitosis having been caused by exposure to the gamma rays of radium a return of mitosis was observed after removal of the radium. Since the completion of the present experiments it has been shown by Spear (6) that by lowering the temperature of tissue cultures to 0° C. for 4 hours and subsequently incubating for various periods there is a fall in the number of cells in mitosis followed by a return in increased numbers which is compensatory.

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