Rocky Mountain Spotted Fever: Protective Value for Guinea Pigs of Vaccine Prepared from Rickettsiae Cultivated in Embryonic Chick Tissues

1939 ◽  
Vol 54 (24) ◽  
pp. 1070 ◽  
Author(s):  
Herald R. Cox
Keyword(s):  
Guinea Pigs ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Embryonic Chick ◽  
Rocky Mountain Spotted Fever

scholarly journals STUDIES ON TYPHUS FEVER

1931 ◽  
Vol 53 (4) ◽  
pp. 493-497 ◽  
Author(s):  
Hans Zinsser ◽  
M. Ruiz Castaneda
Keyword(s):  
Guinea Pigs ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Active Immunization ◽  
Rocky Mountain Spotted Fever ◽  
Typhus Fever

Guinea pigs can be immunized against Mexican typhus virus by peritoneal injections of formalinized Rickettsia material, provided sufficient amounts of the organisms are used. Our results in this respect are analogous to those of Spencer and Parker with carbolized virus of Rocky Mountain spotted fever. The Rickettsia suspensions appear to possess considerable toxicity. We do not wish to be misunderstood as implying that the results in guinea pigs offer anything more than a demonstration of the principle of active immunization with killed Rickettsiae. Application to man will have to be worked out, and preliminary to this, we are now attempting to apply the methods to a limited number of monkeys.

scholarly journals IMMUNITY STUDIES OF ROCKY MOUNTAIN SPOTTED FEVER

1923 ◽  
Vol 38 (5) ◽  
pp. 605-626 ◽  
Author(s):  
Hideyo Noguchi
Keyword(s):  
Experimental Infection ◽  
Guinea Pigs ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Rocky Mountain Spotted Fever ◽  
Refrigerator Temperature ◽  
Considerable Loss ◽  
Immune Rabbit

Freshly prepared mixtures of spotted fever virus and immune rabbit serum in neutral or superneutral proportions confer complete immunity on guinea pigs. The mixtures undergo a considerable loss in immunizing power when heated to 60°C. for 20 minutes, but are still capable, if used in sufficient quantity, of conferring a degree of immunity on the vaccinated animal such that a subsequent experimental infection is rendered less severe and non-fatal. Unheated mixtures which had been preserved in the refrigerator at 4°C. for a period of 32 days still retained a certain degree of immunizing property. The virus alone, or mixed with normal rabbit serum, when allowed to die out by prolonged preservation at refrigerator temperature, or when killed either by heating at 60°C. for 20 minutes or by chemicals (chloroform, ether, xylene) does not induce immunity in guinea pigs.

scholarly journals A CYTOLOGICAL STUDY OF THE NATURE OF RICKETTSIA IN ROCKY MOUNTAIN SPOTTED FEVER

1923 ◽  
Vol 37 (2) ◽  
pp. 221-230 ◽  
Author(s):  
F. M. Nicholson
Keyword(s):  
Endothelial Cells ◽  
Guinea Pigs ◽  
Cytological Study ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
The Other ◽  
Rocky Mountain Spotted Fever ◽  
Nuclear Changes ◽  
Cellular Components ◽  
Blood Pigment

The Rickettsia of Rocky Mountain spotted fever were easily differentiated from mitochondria, phagocytosed blood pigment, nuclear debris, and all other known cellular constituents. Although they were lodged within the cytoplasm of endothelial cells, they were not observed to establish any definite relations with the nucleus or with other cellular components. Their number varied in contiguous cells which sustained the same degree of injury as evidenced by nuclear changes, and alterations in their mitochondria content. The mitochondria, on the other hand, showed similar modifications, characterized by a decrease in number and a rounding up into spherules, in all the endothelial cells seen in a section of an affected blood vessel. Diplobacillary forms were most abundant in the early stages of the reaction and single bacillary bodies towards its termination. Other slight differences in morphology from Wolbach's account were noted in the organisms as seen in the tissues of both ticks and guinea pigs. His study of the distribution of specific lesions with accompanying organisms in the tissues of guinea pigs was confirmed and extended.

scholarly journals New assay of protective activity of Rocky Mountain spotted fever vaccines

1976 ◽  
Vol 4 (3) ◽  
pp. 309-311
Author(s):  
R L Anacker ◽  
R F Smith ◽  
R E Mann ◽  
M A Hamilton
Keyword(s):  
Guinea Pigs ◽  
Spotted Fever ◽  
Dose Range ◽  
Vaccine Dose ◽  
Rocky Mountain ◽  
Protective Activity ◽  
Rocky Mountain Spotted Fever ◽  
Rickettsia Rickettsii

Areas under the fever curves of guinea pigs inoculated with Rocky Mountain spotted fever vaccine over a restricted dose range and infected with a standardized dose of Rickettsia rickettsii varied linearly with log10 dose of vaccine. A calculator was programmed to plot fever curves and calculate the vaccine dose that reduced the fever of infected animals by 50%.

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