Identification in vitro of a Post-Translational Regulatory Site in the Hinge 1 Region of Arabidopsis Nitrate Reductase

1996 ◽  
Vol 8 (3) ◽  
pp. 519
Author(s):  
Wenpei Su ◽  
Steven C. Huber ◽  
Nigel M. Crawford
Keyword(s):  
Nitrate Reductase ◽  
Regulatory Site

In vitro reconstitution of NADH: nitrate reductase in nitrate reductase-deficient mutants of barley

1984 ◽  
Vol 197 (3) ◽  
pp. 358-362 ◽  
Author(s):  
Komaratchi R. Narayanan ◽  
Andreas J. Müller ◽  
Andris Kleinhofs ◽  
Robert L. Warner
Keyword(s):  
Nitrate Reductase ◽  
In Vitro Reconstitution

scholarly journals Membrane-Bound Nitrate Reductase Is Required for Anaerobic Growth in Cystic Fibrosis Sputum

2007 ◽  
Vol 189 (12) ◽  
pp. 4449-4455 ◽  
Author(s):  
Kelli L. Palmer ◽  
Stacie A. Brown ◽  
Marvin Whiteley
Keyword(s):  
Cystic Fibrosis ◽  
Nitrate Reductase ◽  
Mutational Analysis ◽  
Autosomal Recessive Disorder ◽  
Opportunistic Pathogen ◽  
Anaerobic Growth ◽  
Expression Of Genes ◽  
Membrane Bound ◽  
Lung Infections

ABSTRACT The autosomal recessive disorder cystic fibrosis (CF) affects approximately 70,000 people worldwide and is characterized by chronic bacterial lung infections with the opportunistic pathogen Pseudomonas aeruginosa. To form a chronic CF lung infection, P. aeruginosa must grow and proliferate within the CF lung, and the highly viscous sputum within the CF lung provides a likely growth substrate. Recent evidence indicates that anaerobic microenvironments may be present in the CF lung sputum layer. Since anaerobic growth significantly enhances P. aeruginosa biofilm formation and antibiotic resistance, it is important to examine P. aeruginosa physiology and metabolism in anaerobic environments. Measurement of nitrate levels revealed that CF sputum contains sufficient nitrate to support significant P. aeruginosa growth anaerobically, and mutational analysis revealed that the membrane-bound nitrate reductase is essential for P. aeruginosa anaerobic growth in an in vitro CF sputum medium. In addition, expression of genes coding for the membrane-bound nitrate reductase complex is responsive to CF sputum nitrate levels. These findings suggest that the membrane-bound nitrate reductase is critical for P. aeruginosa anaerobic growth with nitrate in the CF lung.

scholarly journals Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.

1992 ◽  
Vol 174 (24) ◽  
pp. 7934-7940 ◽  
Author(s):  
C L Santini ◽  
C Iobbi-Nivol ◽  
C Romane ◽  
D H Boxer ◽  
G Giordano
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
In Vitro Activation

Repair in vitro of nitrate reductase-deficient tobacco mutants (cnxA) by molybdate and by molybdenum cofactor

Planta ◽  
1985 ◽  
Vol 163 (3) ◽  
pp. 370-375 ◽  
Author(s):  
Ralf R. Mendel ◽  
Andreas J. M�ller
Keyword(s):  
Nitrate Reductase ◽  
Molybdenum Cofactor
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