Repair in vitro of nitrate reductase-deficient tobacco mutants (cnxA) by molybdate and by molybdenum cofactor

Planta ◽  
1985 ◽  
Vol 163 (3) ◽  
pp. 370-375 ◽  
Author(s):  
Ralf R. Mendel ◽  
Andreas J. M�ller
Keyword(s):  
Nitrate Reductase ◽  
Molybdenum Cofactor

scholarly journals Biochemical Characterization of Molybdenum Cofactor-free Nitrate Reductase from Neurospora crassa

2013 ◽  
Vol 288 (20) ◽  
pp. 14657-14671 ◽  
Author(s):  
Phillip Ringel ◽  
Joern Krausze ◽  
Joop van den Heuvel ◽  
Ute Curth ◽  
Antonio J. Pierik ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Neurospora Crassa ◽  
Biochemical Characterization ◽  
Nitrate Assimilation ◽  
Molybdenum Cofactor ◽  
Site Formation ◽  
Epr Spectrum ◽  
Redox Active

Nitrate reductase (NR) is a complex molybdenum cofactor (Moco)-dependent homodimeric metalloenzyme that is vitally important for autotrophic organism as it catalyzes the first and rate-limiting step of nitrate assimilation. Beside Moco, eukaryotic NR also binds FAD and heme as additional redox active cofactors, and these are involved in electron transfer from NAD(P)H to the enzyme molybdenum center where reduction of nitrate to nitrite takes place. We report the first biochemical characterization of a Moco-free eukaryotic NR from the fungus Neurospora crassa, documenting that Moco is necessary and sufficient to induce dimer formation. The molybdenum center of NR reconstituted in vitro from apo-NR and Moco showed an EPR spectrum identical to holo-NR. Analysis of mutants unable to bind heme or FAD revealed that insertion of Moco into NR occurs independent from the insertion of any other NR redox cofactor. Furthermore, we showed that at least in vitro the active site formation of NR is an autonomous process.

Identification in vitro of a Post-Translational Regulatory Site in the Hinge 1 Region of Arabidopsis Nitrate Reductase

1996 ◽  
Vol 8 (3) ◽  
pp. 519
Author(s):  
Wenpei Su ◽  
Steven C. Huber ◽  
Nigel M. Crawford
Keyword(s):  
Nitrate Reductase ◽  
Regulatory Site

In vitro reconstitution of NADH: nitrate reductase in nitrate reductase-deficient mutants of barley

1984 ◽  
Vol 197 (3) ◽  
pp. 358-362 ◽  
Author(s):  
Komaratchi R. Narayanan ◽  
Andreas J. Müller ◽  
Andris Kleinhofs ◽  
Robert L. Warner
Keyword(s):  
Nitrate Reductase ◽  
In Vitro Reconstitution
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