A Nodule-Specific Gene Encoding a Subtilisin-Like Protease Is Expressed in Early Stages of Actinorhizal Nodule Development

1995 ◽  
Vol 7 (6) ◽  
pp. 785 ◽  
Author(s):  
Ana Ribeiro ◽  
Antoon D. L. Akkermans ◽  
Ab van Kammen ◽  
Ton Bisseling ◽  
Katharina Pawlowski
Keyword(s):  
Nodule Development ◽  
Specific Gene ◽  
Gene Encoding ◽  
Early Stages ◽  
Actinorhizal Nodule

scholarly journals A nodule-specific gene encoding a subtilisin-like protease is expressed in early stages of actinorhizal nodule development.

1995 ◽  
Vol 7 (6) ◽  
pp. 785-794 ◽  
Author(s):  
A Ribeiro ◽  
A D Akkermans ◽  
A van Kammen ◽  
T Bisseling ◽  
K Pawlowski
Keyword(s):  
Nodule Development ◽  
Specific Gene ◽  
Gene Encoding ◽  
Early Stages ◽  
Actinorhizal Nodule

scholarly journals Stress, novel sex genes, and epigenetic reprogramming orchestrate socially controlled sex change

2019 ◽  
Vol 5 (7) ◽  
pp. eaaw7006 ◽  
Author(s):  
Erica V. Todd ◽  
Oscar Ortega-Recalde ◽  
Hui Liu ◽  
Melissa S. Lamm ◽  
Kim M. Rutherford ◽  
...  
Keyword(s):  
Developmental Process ◽  
Molecular Level ◽  
Sex Change ◽  
Epigenetic Reprogramming ◽  
Stress Axis ◽  
Specific Gene ◽  
Gene Encoding ◽  
Epigenetic Machinery ◽  
Male Sex ◽  
Early Developmental

Bluehead wrasses undergo dramatic, socially cued female-to-male sex change. We apply transcriptomic and methylome approaches in this wild coral reef fish to identify the primary trigger and subsequent molecular cascade of gonadal metamorphosis. Our data suggest that the environmental stimulus is exerted via the stress axis and that repression of the aromatase gene (encoding the enzyme converting androgens to estrogens) triggers a cascaded collapse of feminizing gene expression and identifies notable sex-specific gene neofunctionalization. Furthermore, sex change involves distinct epigenetic reprogramming and an intermediate state with altered epigenetic machinery expression akin to the early developmental cells of mammals. These findings reveal at a molecular level how a normally committed developmental process remains plastic and is reversed to completely alter organ structures.

Molecular characterization of a phloem-specific gene encoding the filament protein, Phloem Protein 1 (PP1), from Cucurbita maxima

1997 ◽  
Vol 12 (1) ◽  
pp. 49-61 ◽  
Author(s):  
Anna M. Clark ◽  
Karin R. Jacobsen ◽  
Dwight E. Bostwick ◽  
Joanne M. Dannenhoffer ◽  
Megan I. Skaggs ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Specific Gene ◽  
Cucurbita Maxima ◽  
Gene Encoding ◽  
Phloem Protein ◽  
Filament Protein

scholarly journals Placenta-specific Expression of the Interleukin-2 (IL-2) Receptor β Subunit from an Endogenous Retroviral Promoter

2011 ◽  
Vol 286 (41) ◽  
pp. 35543-35552 ◽  
Author(s):  
Carla J. Cohen ◽  
Rita Rebollo ◽  
Sonja Babovic ◽  
Elizabeth L. Dai ◽  
Wendy P. Robinson ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Interleukin 2 ◽  
Endogenous Retroviruses ◽  
Specific Gene ◽  
Β Subunit ◽  
Gene Encoding ◽  
Specific Expression ◽  
Tissue Specific ◽  
Cellular Genes ◽  
Villous Trophoblast

The long terminal repeat (LTR) sequences of endogenous retroviruses and retroelements contain promoter elements and are known to form chimeric transcripts with nearby cellular genes. Here we show that an LTR of the THE1D retroelement family has been domesticated as an alternative promoter of human IL2RB, the gene encoding the β subunit of the IL-2 receptor. The LTR promoter confers expression specifically in the placental trophoblast as opposed to its native transcription in the hematopoietic system. Rather than sequence-specific determinants, DNA methylation was found to regulate transcription initiation and splicing efficiency in a tissue-specific manner. Furthermore, we detected the cytoplasmic signaling domain of the IL-2Rβ protein in the placenta, suggesting that IL-2Rβ undergoes preferential proteolytic cleavage in this tissue. These findings implicate novel functions for this cytokine receptor subunit in the villous trophoblast and reveal an intriguing example of ancient LTR exaptation to drive tissue-specific gene expression.

scholarly journals Isolation of a gene encoding a functional zinc finger protein homologous to erythroid Krüppel-like factor: identification of a new multigene family.

1995 ◽  
Vol 15 (11) ◽  
pp. 5957-5965 ◽  
Author(s):  
K P Anderson ◽  
C B Kern ◽  
S C Crable ◽  
J B Lingrel
Keyword(s):  
Dna Binding ◽  
Binding Site ◽  
Zinc Finger ◽  
Reporter Gene ◽  
Zinc Finger Protein ◽  
Erythroid Cell ◽  
Specific Gene ◽  
Beta Globin ◽  
Gene Encoding ◽  
Hybridization Probe

We have identified and characterized the gene for a novel zinc finger transcription factor which we have termed lung Krüppel-like factor (LKLF). LKLF was isolated through the use of the zinc finger domain of erythroid Krüppel-like factor (ELKF) as a hybridization probe and is closely related to this erythroid cell-specific gene. LKLF is expressed in a limited number of tissues, with the predominant expression seen in the lungs and spleen. The gene is developmentally controlled, with expression noted in the 7-day embryo followed by a down-regulation at 11 days and subsequent reactivation. A high degree of similarity is noted in the zinc finger regions of LKLF and EKLF. Beyond this domain, the sequences diverge significantly, although the putative transactivation domains for both LKLF and EKLF are proline-rich regions. In the DNA-binding domain, the three zinc finger motifs are so closely conserved that the predicted DNA contact sites are identical, suggesting that both proteins may bind to the same core sequence. This was further suggested by transactivation assays in which mouse fibroblasts were transiently transfected with a human beta-globin reporter gene in the absence and presence of an LKLF cDNA construct. Expression of the LKLF gene activates this human beta-globin promoter containing the CACCC sequence previously shown to be a binding site for EKLF. Mutation of this potential binding site results in a significant reduction in the reporter gene expression. LKLF and EKLF can thus be grouped as members of a unique family of transcription factors which have discrete patterns of expression in different tissues and which appear to recognize the same DNA-binding site.

Identification of a rat testis-specific gene encoding a potential rat outer dense fibre protein

1998 ◽  
Vol 50 (3) ◽  
pp. 313-322 ◽  
Author(s):  
M.K. O'Bryan ◽  
K.L. Loveland ◽  
D. Herszfeld ◽  
J.R. McFarlane ◽  
M.T.W. Hearn ◽  
...  
Keyword(s):  
Specific Gene ◽  
Gene Encoding ◽  
Rat Testis

scholarly journals A real-time PCR for the differentiation of typhoidal and non-typhoidal Salmonella

2019 ◽  
Author(s):  
Satheesh Nair ◽  
Vineet Patel ◽  
Tadgh Hickey ◽  
Clare Maguire ◽  
David R Greig ◽  
...  
Keyword(s):  
Public Health ◽  
Real Time ◽  
Real Time Pcr ◽  
Genomic Analysis ◽  
Rapid Identification ◽  
Specific Gene ◽  
Whole Genome ◽  
Pcr Assay ◽  
Gene Encoding ◽  
Accurate Identification

AbstractRapid and accurate differentiation of Salmonella spp. causing enteric fever from non-typhoidal Salmonella is essential for clinical management of cases, laboratory risk management and implementation of public health measures. Current methods used for confirmation of identification including biochemistry and serotyping as well as whole genome sequencing analyses, takes several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies, for the rapid identification of typhoidal and non-typhoidal Salmonella.This novel two-hour assay identifies the genus Salmonella by detecting the ttr gene, encoding tetrathionate reductase, and defines typhoidal Salmonella by the detection of S. Typhi and Paratyphi-specific gene combinations. PCR assay performance was determined using 211 clinical cultures of Salmonella (114 non-typhoidal and 97 Typhoidal strains) and 7 clinical non-Salmonella cultures. In addition, the specificity of the assay was evaluated in silico using a diverse in-house collection of 1882 Salmonella whole genome sequences. The real-time PCR results for 218 isolates and the genomic analysis of the 1882 isolates produced 100% sensitivity and 100% specificity (based on a 7 gene profile) for identifying typhoidal Salmonella compared to the Salmonella whole genome sequening identification methods currently used at Public Health England.This paper describes a robust real-time PCR assay for the rapid, accurate identification of typhoidal and non-typhoidal Salmonella which will be invaluable for the urgent screening of isolates from symptomatic individuals, the safe processing of isolates in laboratories and for assisting the management of public health risks.

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