Tissue-Specific Protein Expression in Plant Mitochondria

1994 ◽  
Vol 6 (1) ◽  
pp. 85 ◽  
Author(s):  
Catharine A. Conley ◽  
Maureen R. Hanson
Keyword(s):  
Protein Expression ◽  
Plant Mitochondria ◽  
Specific Protein ◽  
Tissue Specific

scholarly journals Distinct responses to rare codons in select Drosophila tissues

2022 ◽  
Author(s):  
Scott R. Allen ◽  
Rebeccah K Stewart ◽  
Michael Rogers ◽  
Ivan Jimenez Ruiz ◽  
Erez Cohen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Codon Usage ◽  
Codon Usage Bias ◽  
Critical Role ◽  
Specific Protein ◽  
Human Testis ◽  
Rare Codon ◽  
Tissue Specific ◽  
Rare Codons ◽  
Drosophila Testis

Codon usage bias has long been appreciated to influence protein production. Yet, relatively few studies have analyzed the impacts of codon usage on tissue-specific mRNA and protein expression. Here, we use codon-modified reporters to perform an organism-wide screen in Drosophila melanogaster for distinct tissue responses to codon usage bias. These reporters reveal a cliff-like decline of protein expression near the limit of rare codon usage in endogenously expressed Drosophila genes. Near the edge of this limit, however, we find the testis and brain are uniquely capable of expressing rare codon-enriched reporters. We define a new metric of tissue-specific codon usage, the tissue-apparent Codon Adaptation Index, to reveal a conserved enrichment for rare codon usage in the endogenously expressed genes of both Drosophila and human testis. We further demonstrate a role for rare codons in restricting protein expression of an evolutionarily young gene, RpL10Aa, to the Drosophila testis. Rare codon-mediated restriction of this testis-specific protein is critical for female fertility. Our work highlights distinct responses to rarely used codons in select tissues, revealing a critical role for codon bias in tissue biology.

Proteome profiling reveals tissue-specific protein expression in male and female accessory glands of the silkworm, Bombyx mori

Amino Acids ◽  
2016 ◽  
Vol 48 (5) ◽  
pp. 1173-1183 ◽  
Author(s):  
Zhaoming Dong ◽  
Xiaohuan Wang ◽  
Yan Zhang ◽  
Liping Zhang ◽  
Quanmei Chen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Bombyx Mori ◽  
Specific Protein ◽  
Male And Female ◽  
Tissue Specific ◽  
Proteome Profiling ◽  
Accessory Glands ◽  
Silkworm Bombyx Mori

Tissue Distribution and Gender-Specific Protein Expression of Cytochrome P450 in five Mouse Genotypes with a Background of FVB

2018 ◽  
Vol 35 (6) ◽  
Author(s):  
Jiamei M. Chen ◽  
Qisong S. Zhang ◽  
Xiaoyan Y. Li ◽  
Xia Gong ◽  
Yanjiao J. Ruan ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Protein Expression ◽  
Tissue Distribution ◽  
Specific Protein ◽  
And Gender ◽  
Gender Specific

Fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae ofAscaris suum

Parasitology ◽  
2006 ◽  
Vol 133 (4) ◽  
pp. 497-508 ◽  
Author(s):  
M. K. ISLAM ◽  
T. MIYOSHI ◽  
M. YAMADA ◽  
M. A. ALIM ◽  
X. HUANG ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Profiles ◽  
Immunoblot Analysis ◽  
Specific Protein ◽  
Inorganic Pyrophosphatase ◽  
2D Electrophoresis ◽  
Peptide Mass ◽  
Enzyme Families ◽  
Protein Expression Profiles ◽  
Peptide Mass Spectrometry

Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process ofAscaris suumlarvae. This study describes moulting and development-specific protein expression profiles ofA. suumlung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepared for protein analysis using two-dimensional (2D) electrophoresis. NaF exposure inhibited at least 22 proteins in AsLL3 compared with moulted larvae (i.e. AsLL4). A further comparison of AsLL4 with those of pre-cultured AsLL3 and NaF-exposed AsLL3 revealed 8 stage-specifically and 4 over-expressed proteins. Immunoblot analysis revealed an inhibition by NaF of 19 immunoreactive proteins. Enzyme assay and immunochemical data showed an inhibition of the moulting-specific inorganic pyrophosphatase activity by 41% and a decreased expression in NaF-treated larvae, indicating its significance in the moulting process. A protein spot associated with NaF inhibition was isolated and identified by peptide mass spectrometry and bioinformatics approaches to be a member of 3–hydroxyacyl–CoA dehydrogenase/short-chain dehydrogenase enzyme families. These results have implications for the identification of proteins specific to the moulting process as potential chemotherapeutic targets.

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