Characterization of cis-Acting Sequences Regulating Root-Specific Gene Expression in Tobacco

1991 ◽  
Vol 3 (4) ◽  
pp. 371 ◽  
Author(s):  
Yuri T. Yamamoto ◽  
Christopher G. Taylor ◽  
Gregoria N. Acedo ◽  
Chi-Lien Cheng ◽  
Mark A. Conkling
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Cis Acting ◽  
Specific Gene Expression

scholarly journals Characterization of cis-acting sequences regulating root-specific gene expression in tobacco.

1991 ◽  
Vol 3 (4) ◽  
pp. 371-382 ◽  
Author(s):  
Y T Yamamoto ◽  
C G Taylor ◽  
G N Acedo ◽  
C L Cheng ◽  
M A Conkling
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Cis Acting ◽  
Specific Gene Expression

Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression

1992 ◽  
Vol 12 (3) ◽  
pp. 1202-1208
Author(s):  
R A Graves ◽  
P Tontonoz ◽  
B M Spiegelman
Keyword(s):  
Gene Expression ◽  
Cultured Cells ◽  
Mutational Analysis ◽  
Cell Types ◽  
Specific Gene ◽  
Enhancer Activity ◽  
Cis Acting ◽  
Specific Gene Expression ◽  
Nuclear Extracts ◽  
Adipogenic Gene

The molecular basis of adipocyte-specific gene expression is not well understood. We have previously identified a 518-bp enhancer from the adipocyte P2 gene that stimulates adipose-specific gene expression in both cultured cells and transgenic mice. In this analysis of the enhancer, we have defined and characterized a 122-bp DNA fragment that directs differentiation-dependent gene expression in cultured preadipocytes and adipocytes. Several cis-acting elements have been identified and shown by mutational analysis to be important for full enhancer activity. One pair of sequences, ARE2 and ARE4, binds a nuclear factor (ARF2) present in extracts derived from many cell types. Multiple copies of these elements stimulate gene expression from a minimal promoter in preadipocytes, adipocytes, and several other cultured cell lines. A second pair of elements, ARE6 and ARE7, binds a separate factor (ARF6) that is detected only in nuclear extracts derived from adipocytes. The ability of multimers of ARE6 or ARE7 to stimulate promoter activity is strictly adipocyte specific. Mutations in the ARE6 sequence greatly reduce the activity of the 518-bp enhancer. These data demonstrate that several cis- and trans-acting components contribute to the activity of the adipocyte P2 enhancer and suggest that ARF6, a novel differentiation-dependent factor, may be a key regulator of adipogenic gene expression.

Generation and characterization of variants of mouse hepatoma cells with defects in hepato-specific gene expression. I. Albumin synthesis variants

1982 ◽  
Vol 8 (4) ◽  
pp. 451-464 ◽  
Author(s):  
Gretchen J. Darlington ◽  
John Papaconstantinou ◽  
David W. Sammons ◽  
Peter C. Brown ◽  
Edith Y. Wong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatoma Cells ◽  
Specific Gene ◽  
Albumin Synthesis ◽  
Specific Gene Expression ◽  
Mouse Hepatoma

scholarly journals Functional analysis of the endothelial cell-specific Tie2/Tek promoter identifies unique protein-binding elements

1998 ◽  
Vol 330 (1) ◽  
pp. 335-343 ◽  
Author(s):  
M. Bahaa FADEL ◽  
C. Stephane BOUTET ◽  
Thomas QUERTERMOUS
Keyword(s):  
Gene Expression ◽  
Endothelial Cell ◽  
Dna Binding ◽  
Protein Binding ◽  
Dna Sequences ◽  
Molecular Basis ◽  
Proximal Promoter ◽  
Specific Gene ◽  
Specific Gene Expression

To investigate the molecular basis of endothelial cell-specific gene expression, we have examined the DNA sequences and the cognate DNA-binding proteins that mediate transcription of the murine tie2/tek gene. Reporter transfection experiments conformed with earlier findings in transgenic mice, indicating that the upstream promoter of Tie2/Tek is capable of activating transcription in an endothelial cell-specific fashion. These experiments have also allowed the identification of a single upstream inhibitory region (region I) and two positive regulatory regions (regions U and A) in the proximal promoter. Electrophoretic mobility-shift assays have allowed further characterization of three novel DNA-binding sequences associated with these regions and have provided preliminary characterization of the protein factors binding to these elements. Two of the elements (U and A) confer increased transcription on a heterologous promoter, with element U functioning in an endothelial-cell-selective manner. By employing embryonic endothelial-like yolk sac cells in parallel with adult-derived endothelial cells, we have identified differences in functional activity and protein binding that may reflect mechanisms for specifying developmental regulation of tie2/tek expression. Further study of the DNA and protein elements characterized in these experiments is likely to provide new insight into the molecular basis of developmental- and cell-specific gene expression in the endothelium.

scholarly journals Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression.

1992 ◽  
Vol 12 (3) ◽  
pp. 1202-1208 ◽  
Author(s):  
R A Graves ◽  
P Tontonoz ◽  
B M Spiegelman
Keyword(s):  
Gene Expression ◽  
Cultured Cells ◽  
Mutational Analysis ◽  
Cell Types ◽  
Specific Gene ◽  
Enhancer Activity ◽  
Cis Acting ◽  
Specific Gene Expression ◽  
Nuclear Extracts ◽  
Adipogenic Gene

The molecular basis of adipocyte-specific gene expression is not well understood. We have previously identified a 518-bp enhancer from the adipocyte P2 gene that stimulates adipose-specific gene expression in both cultured cells and transgenic mice. In this analysis of the enhancer, we have defined and characterized a 122-bp DNA fragment that directs differentiation-dependent gene expression in cultured preadipocytes and adipocytes. Several cis-acting elements have been identified and shown by mutational analysis to be important for full enhancer activity. One pair of sequences, ARE2 and ARE4, binds a nuclear factor (ARF2) present in extracts derived from many cell types. Multiple copies of these elements stimulate gene expression from a minimal promoter in preadipocytes, adipocytes, and several other cultured cell lines. A second pair of elements, ARE6 and ARE7, binds a separate factor (ARF6) that is detected only in nuclear extracts derived from adipocytes. The ability of multimers of ARE6 or ARE7 to stimulate promoter activity is strictly adipocyte specific. Mutations in the ARE6 sequence greatly reduce the activity of the 518-bp enhancer. These data demonstrate that several cis- and trans-acting components contribute to the activity of the adipocyte P2 enhancer and suggest that ARF6, a novel differentiation-dependent factor, may be a key regulator of adipogenic gene expression.

scholarly journals Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei

2008 ◽  
Vol 147 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Changqing Zhang ◽  
Roger A. Barthelson ◽  
Georgina M. Lambert ◽  
David W. Galbraith
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Specific Gene Expression
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