scholarly journals Identification and functional characterization of cataract-specific gene expression changes reveals important pathways for human lens maintenance, aging and disease

2004 ◽  
Author(s):  
John R. Hawse
Keyword(s):  
Gene Expression ◽  
Functional Characterization ◽  
Human Lens ◽  
Specific Gene ◽  
Specific Gene Expression

Generation and characterization of variants of mouse hepatoma cells with defects in hepato-specific gene expression. I. Albumin synthesis variants

1982 ◽  
Vol 8 (4) ◽  
pp. 451-464 ◽  
Author(s):  
Gretchen J. Darlington ◽  
John Papaconstantinou ◽  
David W. Sammons ◽  
Peter C. Brown ◽  
Edith Y. Wong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatoma Cells ◽  
Specific Gene ◽  
Albumin Synthesis ◽  
Specific Gene Expression ◽  
Mouse Hepatoma

scholarly journals Functional analysis of the endothelial cell-specific Tie2/Tek promoter identifies unique protein-binding elements

1998 ◽  
Vol 330 (1) ◽  
pp. 335-343 ◽  
Author(s):  
M. Bahaa FADEL ◽  
C. Stephane BOUTET ◽  
Thomas QUERTERMOUS
Keyword(s):  
Gene Expression ◽  
Endothelial Cell ◽  
Dna Binding ◽  
Protein Binding ◽  
Dna Sequences ◽  
Molecular Basis ◽  
Proximal Promoter ◽  
Specific Gene ◽  
Specific Gene Expression

To investigate the molecular basis of endothelial cell-specific gene expression, we have examined the DNA sequences and the cognate DNA-binding proteins that mediate transcription of the murine tie2/tek gene. Reporter transfection experiments conformed with earlier findings in transgenic mice, indicating that the upstream promoter of Tie2/Tek is capable of activating transcription in an endothelial cell-specific fashion. These experiments have also allowed the identification of a single upstream inhibitory region (region I) and two positive regulatory regions (regions U and A) in the proximal promoter. Electrophoretic mobility-shift assays have allowed further characterization of three novel DNA-binding sequences associated with these regions and have provided preliminary characterization of the protein factors binding to these elements. Two of the elements (U and A) confer increased transcription on a heterologous promoter, with element U functioning in an endothelial-cell-selective manner. By employing embryonic endothelial-like yolk sac cells in parallel with adult-derived endothelial cells, we have identified differences in functional activity and protein binding that may reflect mechanisms for specifying developmental regulation of tie2/tek expression. Further study of the DNA and protein elements characterized in these experiments is likely to provide new insight into the molecular basis of developmental- and cell-specific gene expression in the endothelium.

scholarly journals Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei

2008 ◽  
Vol 147 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Changqing Zhang ◽  
Roger A. Barthelson ◽  
Georgina M. Lambert ◽  
David W. Galbraith
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals Characterization of the Promoter Controling the Stage-Specific Gene Expression of Bombyx mori

2011 ◽  
Vol 21 (10) ◽  
pp. 1466-1472 ◽  
Author(s):  
Seung-Won Park ◽  
Gwang-Ho Choi ◽  
Tae-Won Goo ◽  
Seong-Ryul Kim ◽  
Seok-Woo Kang
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals Characterization of cis elements of the probasin promoter necessary for prostate-specific gene expression

The Prostate ◽  
2010 ◽  
Vol 70 (9) ◽  
pp. 934-951 ◽  
Author(s):  
JianFeng Zhang ◽  
Nan Gao ◽  
David J. DeGraff ◽  
Xiuping Yu ◽  
Qian Sun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Cis Elements ◽  
Specific Gene Expression
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