A Cellophane Tape-Cover Glass Technique for Preparing Microscopic Slide Mounts of Fungi

Mycologia ◽  
1966 ◽  
Vol 58 (4) ◽  
pp. 655 ◽  
Author(s):  
Robert M. Endo
Keyword(s):  
Cover Glass ◽  
Cellophane Tape ◽  
Microscopic Slide

Comparison of Virus Recovery Techniques for Assessing the Virucidal Activity of Disinfectant Spray Products

1975 ◽  
Vol 58 (1) ◽  
pp. 130-132
Author(s):  
John H S Chen
Keyword(s):  
Test Methods ◽  
Virucidal Activity ◽  
Cotton Swab ◽  
Cover Glass ◽  
Log Reduction ◽  
Recovery Techniques ◽  
The Mean ◽  
Virus Recovery ◽  
Glass Surfaces ◽  
Microscopic Slide

Abstract A comparison was made of virus recovery from inoculated glass surfaces by 3 different viral recovery techniques used in spray test methods. On a total of 7, 5, and 5 observations, for the cover glass crushing, cotton swab rubbing, and microscopic slide shaking techniques, respectively, the log reduction of the median tissue culture infective dose (log TCID50/ml) averaged 1.48, 2.57, and 2.22, the mean percentage recovery was 77.98, 61.55, and 66.94%, and the coefficient of variation was 4.37, 10.01, and 7.42%. The cover glass technique was found to be the least variable and the most effective and precise of the 3 methods.

Aspiration cytology and transmission electron microscopy (TEM): A novel tissue-collection technique with useful diagnostic applications

1984 ◽  
Vol 42 ◽  
pp. 90-93
Author(s):  
J.A. Maksem ◽  
C. VanDyke ◽  
H.W. Carter ◽  
C.F. Galang
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Diagnostic Technique ◽  
Needle Aspiration ◽  
Large Series ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Transmission Electron ◽  
Diagnostic Applications ◽  
Microscopic Slide

In the last decade fine needle aspiraration biopsy has gained recognition as a valuable diagnostic technique, and its benefits have been demonstrated in large series of patients with almost every type of tumor (1,2). The usual way to collect cellular material from needle-aspiration biopsies is to discharge the needle and syringe contents onto a microscopic slide and smear the material with another slide. The entire specimen is contained on the slides prepared at the time of biopsy. Serious technical difficulties are inherent to this method. 1) Inconsistent fixation, 2) drying artifact, 3) loss of tissue fragments, 4) inability to confirm impressions by a “second method”, and 5) retention of significant diagnostic material in the needle hub. Our technique avoids these difficulties.

Flat embeddment of monolayer cells, tissue sections, and other cellular materials attached onto glass substrate

1990 ◽  
Vol 48 (3) ◽  
pp. 766-767
Author(s):  
Jeffrey P. Chang ◽  
Jaang J. Wang
Keyword(s):  
Present Report ◽  
Cellular Materials ◽  
Cover Glass ◽  
Tissue Sections ◽  
Embedding Technique ◽  
Exfoliated Cells ◽  
Black Paper ◽  
Cell Concentrations ◽  
Flat Embedding

Flat embeddment of certain specimens for electron microscopy is necessary for three classes of biological materials: namely monolayer cells, tissue sections of paraffin or plastics, as well as cell concentrations, exfoliated cells, and cell smears. The present report concerns a flat-embedding technique which can be applied to all these three classes of materials and which is a modified and improved version of Chang's original methodology.Preparation of coverglasses and microslides. Chemically cleaned coverglasses, 11 × 22 mm or other sizes, are laid in rows on black paper. Ink-mark one coner for identifying the spray-side of the glass for growing cells. Lightly spray with Teflon monomer (Heddy/Contact Inductries, Paterson, NO 07524, U.S.A.) from a pressurized can. Bake the sprayed glasses at 500°F for 45 min on Cover-Glass Ceramic Racks (A. Thomas Co. Philadelphia), for Teflon to polymerize.Monolayer Cells. After sterilization, the Teflon-treated coverglasses, with cells attached, are treated or fixed in situ in Columbia staining dishes (A. Thomas Co., Philadelphia) for subsequent processing.

scholarly journals III. On the coalescence of amœboid cells into plasmodia, and on the so-called coagulation of invertebrate fluids

1880 ◽  
Vol 30 (200-205) ◽  
pp. 252-255 ◽  
Keyword(s):  
Cover Glass ◽  
Superficial Resemblance ◽  
Glass Cell ◽  
Amoeboid Cells

Whether one collects the perivisceral fluid of a sea-urehin or of a worm, or the blood of a crustacean or a mollusc, the same phenomenon is always more or less distinctly to be observed. A kind of coagulation takes place, the fluid separating sooner or later into two portions, which have considerable superficial resemblance to the clot and serum of vertebrate blood. It is easy to watch the formation of the clot by placing a drop of fresh-drawn fluid upon a cover-glass and inverting this above a glass cell, of which the edge is oiled to prevent evaporation. The drop thus hangs freely and the coagulation can go on without interference.

scholarly journals Inactivation of Prions by Low-Temperature Sterilization Technology Using Vaporized Gas Derived from a Hydrogen Peroxide–Peracetic Acid Mixture

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 24
Author(s):  
Akikazu Sakudo ◽  
Daiki Anraku ◽  
Tomomasa Itarashiki
Keyword(s):  
Hydrogen Peroxide ◽  
Medical Devices ◽  
Neurodegenerative Disorders ◽  
Peracetic Acid ◽  
Protein Misfolding ◽  
Prion Diseases ◽  
Proteinase K ◽  
Acid Mixture ◽  
Cover Glass ◽  
Standard Mode

Prion diseases are proteopathies that cause neurodegenerative disorders in humans and animals. Prion is highly resistant to both chemical and physical inactivation. Here, vaporized gas derived from a hydrogen peroxide–peracetic acid mixture (VHPPA) was evaluated for its ability to inactivate prion using a STERIACE 100 instrument (Saraya Co., Ltd.). Brain homogenates of scrapie (Chandler strain) prion-infected mice were placed on a cover glass, air-dried, sealed in a Tyvek package, and subjected to VHPPA treatment at 50–55 °C using 8% hydrogen peroxide and <10% peracetic acid for 47 min (standard mode, SD) or 30 min (quick mode, QC). Untreated control samples were prepared in the same way but without VHPPA. The resulting samples were treated with proteinase K (PK) to separate PK-resistant prion protein (PrPres), as a marker of the abnormal isoform (PrPSc). Immunoblotting showed that PrPres was reduced by both SD and QC VHPPA treatments. PrPres bands were detected after protein misfolding cyclic amplification of control but not VHPPA-treated samples. In mice injected with prion samples, VHPPA treatment of prion significantly prolonged survival relative to untreated samples, suggesting that it decreases prion infectivity. Taken together, the results show that VHPPA inactivates prions and might be applied to the sterilization of contaminated heat-sensitive medical devices.

A Novel Cell Co-Culture Method Utilizing Thermo-Responsive Surface

2007 ◽  
Vol 342-343 ◽  
pp. 221-224
Author(s):  
Jin Suk Bae ◽  
Ga Young Jun ◽  
Akihiko Kikuchi ◽  
Teruo Okano ◽  
Chang Hyun Ahn ◽  
...  
Keyword(s):  
Contact Angle ◽  
Electron Spectroscopy ◽  
Electron Beam Irradiation ◽  
Culture Method ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Beam Irradiation ◽  
Cover Glass ◽  
Force Microscopy ◽  
Atomic Force

In this work, we developed a novel patterned co-culture method with thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) and poly(N-ρ-vinylbenzyl-Ο-β-D-galactopyranosyl-(1→ 4)-D-gluconamide) (PVLA) inducing active hepatocyte attachment. Patterned graft of PIPAAm onto PS dishes was carried out by electron beam irradiation using cover-glass as a photomask. PVLA was only coated onto PIPAAm-ungrafted domain because of hydrated hydrophilic property of PIPAAm at below the LCST. Analysis by attenuated total reflection-Fourier transform infrared and electron spectroscopy for chemical analysis revealed that PIPAAm and PVLA were successfully grafted and coated on surfaces of PS dishes. PIPAAm-grafted surface exhibited decreasing contact angle by changing temperature from 37 to 20°C, while PVLA-coated PS and non-treated PS had negligible contact angle changes with temperature alternation. Atomic force microscopy (AFM) results showed that PIPAAm-grafted and PVLA-coated PS had smoother surfaces than that of ungrafted PS dishes. After culture for 12 hours, hepatocytes were well attached on PVLA-coated domain. Hepatocytes adherent on PIPAAm-grafted domain were detached by decreasing temperature. And then, fibroblasts were seeded onto PIPAAm pattern-grafted domain. Fibroblasts were only attached and spread onto PIPAAm-grafted domain. Co-cultured hepatocytes showed better differentiated function of albumin expression compared to homotypic hepatocyte culture

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