Oncogenic Transformation of C3H 10T1/2 Cells Exposed to Alpha Particles: Sensitivity through the Cell Cycle

1995 ◽  
Vol 142 (3) ◽  
pp. 276 ◽  
Author(s):  
D. Bettega ◽  
P. Calzolari ◽  
A. Costa ◽  
G. Noris Chiorda ◽  
L. Tallone
Keyword(s):  
Cell Cycle ◽  
Alpha Particles ◽  
Oncogenic Transformation

Neutron-Induced Cell Cycle-Dependent Oncogenic Transformation of C3H 10T1/2 Cells

1995 ◽  
Vol 142 (3) ◽  
pp. 270 ◽  
Author(s):  
R. C. Miller ◽  
C. R. Geard ◽  
S. G. Martin ◽  
S. A. Marino ◽  
E. J. Hall
Keyword(s):  
Cell Cycle ◽  
Oncogenic Transformation ◽  
Cycle Dependent

scholarly journals v-Src-Induced Modulation of the Calpain-Calpastatin Proteolytic System Regulates Transformation

2002 ◽  
Vol 22 (1) ◽  
pp. 257-269 ◽  
Author(s):  
N. O. Carragher ◽  
M. A. Westhoff ◽  
D. Riley ◽  
D. A. Potter ◽  
P. Dutt ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Focal Adhesion ◽  
Cell Cycle Progression ◽  
Cell Transformation ◽  
Proteolytic Cleavage ◽  
Cell Phenotype ◽  
Oncogenic Transformation ◽  
Morphological Transformation ◽  
Proteolytic System ◽  
Anchorage Independent Growth

ABSTRACT v-Src-induced oncogenic transformation is characterized by alterations in cell morphology, adhesion, motility, survival, and proliferation. To further elucidate some of the signaling pathways downstream of v-Src that are responsible for the transformed cell phenotype, we have investigated the role that the calpain-calpastatin proteolytic system plays during oncogenic transformation induced by v-Src. We recently reported that v-Src-induced transformation of chicken embryo fibroblasts is accompanied by calpain-mediated proteolytic cleavage of the focal adhesion kinase (FAK) and disassembly of the focal adhesion complex. In this study we have characterized a positive feedback loop whereby activation of v-Src increases protein synthesis of calpain II, resulting in degradation of its endogenous inhibitor calpastatin. Reconstitution of calpastatin levels by overexpression of exogenous calpastatin suppresses proteolytic cleavage of FAK, morphological transformation, and anchorage-independent growth. Furthermore, calpastatin overexpression represses progression of v-Src-transformed cells through the G1 stage of the cell cycle, which correlates with decreased pRb phosphorylation and decreased levels of cyclins A and D and cyclin-dependent kinase 2. Calpain 4 knockout fibroblasts also exhibit impaired v-Src-induced morphological transformation and anchorage-independent growth. Thus, modulation of the calpain-calpastatin proteolytic system plays an important role in focal adhesion disassembly, morphological transformation, and cell cycle progression during v-Src-induced cell transformation.

Deregulation of Cell Cycle Progression by Oncogenic Transformation

2003 ◽  
pp. 133-152
Author(s):  
Marcelo L. Rodriguez-Puebla ◽  
Adrian M. Senderowicz ◽  
Claudio J. Conti
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Oncogenic Transformation ◽  
Cycle Progression

The Biological Effectiveness of Radon-Progeny Alpha Particles. II. Oncogenic Transformation as a Function of Linear Energy Transfer

1995 ◽  
Vol 142 (1) ◽  
pp. 54 ◽  
Author(s):  
Richard C. Miller ◽  
Stephen A. Marino ◽  
David J. Brenner ◽  
Stewart G. Martin ◽  
Marcia Richards ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Linear Energy Transfer ◽  
Alpha Particles ◽  
Radon Progeny ◽  
Oncogenic Transformation ◽  
Biological Effectiveness

Alterations in the Progression of Cells through the Cell Cycle after Exposure to Alpha Particles or Gamma Rays

1996 ◽  
Vol 146 (4) ◽  
pp. 414 ◽  
Author(s):  
Donna M. Gadbois ◽  
Harry A. Crissman ◽  
Anthony Nastasi ◽  
Robb Habbersett ◽  
Sha-ke Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Gamma Rays ◽  
Alpha Particles

Cell-Cycle-Dependent Radiation-Induced Oncogenic Transformation of C3H 10T1/2 Cells

1992 ◽  
Vol 130 (1) ◽  
pp. 129 ◽  
Author(s):  
Richard C. Miller ◽  
Charles R. Geard ◽  
Marcus J. Geard ◽  
Eric J. Hall
Keyword(s):  
Cell Cycle ◽  
Oncogenic Transformation ◽  
Radiation Induced ◽  
Cycle Dependent

Oncogenic Transformation of Mouse BALB/3T3 Cells by Plutonium-238 Alpha Particles

1983 ◽  
Vol 96 (2) ◽  
pp. 261 ◽  
Author(s):  
James B. Robertson ◽  
Andreas Koehler ◽  
Jacquelyn George ◽  
John B. Little
Keyword(s):  
Alpha Particles ◽  
Oncogenic Transformation ◽  
3T3 Cells
Sign in / Sign up

Export Citation Format

Share Document