A Charge-Coupled-Device Camera Image Analysis System for Quantifying DNA Distributions in Agarose Gels after Pulsed-Field Gel Electrophoresis

1994 ◽  
Vol 140 (1) ◽  
pp. 37 ◽  
Author(s):  
W. C. Dewey ◽  
L. L. Thompson ◽  
M. L. Trinh ◽  
D. L. Latz ◽  
J. F. Ward
Author(s):  
Yassin Labyed ◽  
Naima Kaabouch ◽  
Richard R. Schultz ◽  
Brij B. Singh ◽  
Barry Milavetz

Author(s):  
D.S. DeMiglio

Much progress has been made in recent years towards the development of closed-loop foundry sand reclamation systems. However, virtually all work to date has determined the effectiveness of these systems to remove surface clay and metal oxide scales by a qualitative inspection of a representative sampling of sand particles. In this investigation, particles from a series of foundry sands were sized and chemically classified by a Lemont image analysis system (which was interfaced with an SEM and an X-ray energy dispersive spectrometer) in order to statistically document the effectiveness of a reclamation system developed by The Pangborn Company - a subsidiary of SOHIO.The following samples were submitted: unreclaimed sand; calcined sand; calcined & mechanically scrubbed sand and unused sand. Prior to analysis, each sample was sprinkled onto a carbon mount and coated with an evaporated film of carbon. A backscattered electron photomicrograph of a field of scale-covered particles is shown in Figure 1. Due to a large atomic number difference between sand particles and the carbon mount, the backscattered electron signal was used for image analysis since it had a uniform contrast over the shape of each particle.


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