Polymorphism in DNA restriction patterns of Coxiella burnetii isolates investigated by pulsed field gel electrophoresis and image analysis

1993 ◽  
Vol 9 (4) ◽  
pp. 419-425 ◽  
Author(s):  
D. Thiele ◽  
H. Willems ◽  
G. Köpf ◽  
H. Krauss
Keyword(s):  
Image Analysis ◽  
Coxiella Burnetii ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Dna Restriction

Pulsed-field gel electrophoresis applied for comparing Listeria monocytogenes strains involved in outbreaks

1993 ◽  
Vol 39 (4) ◽  
pp. 395-401 ◽  
Author(s):  
C. Buchrieser ◽  
R. Brosch ◽  
B. Catimel ◽  
J. Rocourt
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Chromosomal Dna ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks., We used pulsed-field gel electrophoresis of large chromosomal DNA restriction fragments generated by ApaI, SmaI, or NotI to analyse 75 L. monocytogenes strains isolated during six major and eight smaller recent listeriosis outbreaks. These strains could be divided into 20 different genomic varieties. Thirteen of 14 strains isolated during major epidemics in Switzerland (1983–1987), the United States (California, 1985) and Denmark (1985–1987) demonstrated indistinguishable DNA restriction patterns. In contrast, strains responsible for the outbreaks in Canada (Nova Scotia, 1981), the United States (Massachusetts, 1983), France (Anjou, 1975–1976), New Zealand (1969), and Austria (1986) and some smaller outbreaks in France (1987, 1988, 1989) were each characterized by particular combinations of DNA restriction patterns. Seventy-seven percent of the tested strains could be classified into the previously described ApaI group A (Brosch et al. 1991), demonstrating a very close genomic relatedness. Because 49% of the epidemic strains selected for this study belonged to phagovar 2389/2425/3274/2671/47/108/340 or 2389/47/108/340, fifty-six additional strains of these phagovars, isolated from various origins, were also typed to determine whether differences in DNA restriction profiles between epidemic and randomly selected strains of the same phagovars could be pointed out. Variations in DNA patterns appeared more frequently within randomly selected strains than within epidemic strains.Key words: Listeria monocytogenes, listeriosis, typing, pulsed-field gel electrophoresis, epidemic.

scholarly journals DNA restriction patterns produced by pulsed-field gel electrophoresis in Moraxella catarrhalis isolated from different geographical areas

1999 ◽  
Vol 122 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. MARTINEZ ◽  
K. AHMED ◽  
C. H. ZHENG ◽  
K. WATANABE ◽  
K. OISHI ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Moraxella Catarrhalis ◽  
Genomic Dna ◽  
Respiratory Infections ◽  
Pulsed Field Gel Electrophoresis ◽  
Dynamic Changes ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Time Period ◽  
Dna Restriction

Pulsed field gel electrophoresis (PFGE) of the genomic DNA of Moraxella catarrhalis was done in 172 strains isolated from sputum of patients with respiratory infections in Nagasaki (130 strains), Europe (14 strains), Thailand (6 strains), Uganda (3 strains), Bangladesh (5 strains) and Kuwait (14 strains). Restriction endonuclease with SmaI generated 4–16 DNA fragments ranging from 1000 kb to 24·25 kb and was classified into 31 major groups. It was found that there were wide variations of DNA restriction patterns of strains isolated from the same and different geographical areas. DNA restriction patterns of strains isolated in Nagasaki during the last 12 years showed dynamic changes of the predominant strains in each time period. We conclude from this study that PFGE is a suitable method to document interstrain variation in M. catarrhalis.

Use of Pulsed Field Gel Electrophoresis to Differentiate Coxiella burnetii Strains

1990 ◽  
Vol 590 (1 Rickettsiolog) ◽  
pp. 504-513 ◽  
Author(s):  
R. HEINZEN ◽  
G. L. STIEGLER ◽  
L. L. WHITING ◽  
S. A. SCHMITT ◽  
L. P. MALLAVIA ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Comparative Analysis of Infrequent-Restriction-Site PCR and Pulsed-Field Gel Electrophoresis for Epidemiological Typing ofLegionella pneumophila Serogroup 1 Strains

1998 ◽  
Vol 36 (1) ◽  
pp. 161-167 ◽  
Author(s):  
Serge Riffard ◽  
François Lo Presti ◽  
François Vandenesch ◽  
Françoise Forey ◽  
Monique Reyrolle ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Gel Electrophoresis ◽  
Restriction Site ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Analysis ◽  
Pcr Assay ◽  
Pulsed Field ◽  
Restriction Sites ◽  
Dna Restriction ◽  
High Level

Two methods were compared for the analysis of 48 unrelated and epidemiologically related Legionella pneumophila serogroup 1 isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI restriction sites and the pulsed-field gel electrophoresis (PFGE) analysis determined after DNA restriction with SfiI. Both methods demonstrated a high level of discrimination with a similar capacity for differentiating 23 of the 24 unrelated isolates. PFGE analysis and IRS-PCR assay were both able to identify epidemiologically related isolates of L. pneumophila from three outbreaks. Hence, IRS-PCR assay appears to be a reproducible (intergel reproducibility, 100%) and discriminative (discriminatory index, ≥0.996) tool for typing of Legionella. Compared to PFGE, however, IRS-PCR presented an advantage through ease of performance and with attributes of rapidity and sensitivity of target DNA.

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