18 S Ribosomal RNA Is Degraded during Ribosome Maturation in Irradiated HeLa Cells

1989 ◽  
Vol 118 (2) ◽  
pp. 330 ◽  
Author(s):  
John M. Krolak ◽  
David McClain ◽  
Stephen L. Snyder ◽  
Pinhas Fuchs ◽  
Kenneth W. Minton
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
18 S Ribosomal Rna

scholarly journals Rcl1 Protein, a Novel Nuclease for 18 S Ribosomal RNA Production

2011 ◽  
Vol 286 (39) ◽  
pp. 34082-34087 ◽  
Author(s):  
Darryl M. Horn ◽  
Saundra L. Mason ◽  
Katrin Karbstein
Keyword(s):  
Ribosomal Rna ◽  
Protein A ◽  
18 S Ribosomal Rna

Synthesis of Ribosomal RNA in Synchronized Hela Cells

Nature ◽  
1965 ◽  
Vol 208 (5009) ◽  
pp. 464-466 ◽  
Author(s):  
MATTHEW D. SCHARFF ◽  
ELLIOTT ROBBINS
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells

Nuclear metabolism of ribosomal RNA in growing, methionine-limited, and ethionine-treated HeLa cells

Biochemistry ◽  
1977 ◽  
Vol 16 (12) ◽  
pp. 2783-2791 ◽  
Author(s):  
Stanley F. Wolf ◽  
David Schlessinger
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells

scholarly journals The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids

1968 ◽  
Vol 107 (6) ◽  
pp. 851-859 ◽  
Author(s):  
M. L. Fenwick
Keyword(s):  
Hydrogen Bonding ◽  
Ribosomal Rna ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Linear Structure ◽  
Sedimentation Rates ◽  
Amino Groups ◽  
Ribonucleic Acids ◽  
Molecular Weights ◽  
Virus Rna

It has been reported that the RNA of several bacteriophages and that of the larger ribosomal sub-units of mammalian cells sediment faster in the presence of 0·1m-sodium chloride than is expected from their estimated molecular weights. The effect of blocking the hydrogen-bonding amino groups of these and other types of RNA was studied. The RNA of phage R17 no longer sedimented anomalously fast after treatment with formaldehyde. In contrast, the larger ribosomal RNA of HeLa cells appeared more aberrant than before, sedimenting faster than tobacco-mosaic-virus RNA (mol.wt. 2×106) in the presence of formaldehyde. The rapidly labelled nuclear 45s RNA of HeLa cells still sedimented faster than the larger ribosomal RNA after reaction with formaldehyde, showing no evidence of disaggregation. It is suggested that both the large ribosomal RNA and the 45s RNA of HeLa cells may have a non-linear structure.

scholarly journals Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable.

1981 ◽  
Vol 1 (6) ◽  
pp. 497-511 ◽  
Author(s):  
R Gelfand ◽  
G Attardi
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
Half Life ◽  
Messenger Rna ◽  
Specific Activity ◽  
Mitochondrial Rna ◽  
Messenger Rnas ◽  
Precursor Pool ◽  
Messenger Ribonucleic Acid

The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes in specific activity of the mitochondrial nucleotide precursor pools. In one experiment, a novel method for determining the nucleotide precursor pool specific activities, using nascent RNA chains, has been utilized. All mitochondrial RNA species analyzed were found to be metabolically unstable, with half-lives of 2.5 to 3.5 h for the two ribosomal RNA components and between 25 and 90 min for the various putative messenger RNAs. A cordycepin "chase" experiment yielded half-life values for the messenger RNA species which were, in general, larger by a factor of 1.5 to 2.5 than those estimated in the labeling kinetics experiments. On the basis of previous observations, a model is proposed whereby the rate of mitochondrial RNA decay is under feedback control by some mechanism linked to RNA synthesis or processing. A short half-life was determined for five large polyadenylated RNAs, which are probably precursors of mature species. A rate of synthesis of one to two molecules per minute per cell was estimated for the various H-strand-coded messenger RNA species, and a rate of synthesis 50 to 100 times higher was estimated for the ribosomal RNA species. These data indicate that the major portion of the H-strand in each mitochondrial deoxyribonucleic acid molecule is transcribed very infrequently, possibly as rarely as once or twice per cell generation. Furthermore, these results are consistent with a previously proposed model of H-strand transcription in the form of a single polycistronic molecule.

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