Nuclear metabolism of ribosomal RNA in growing, methionine-limited, and ethionine-treated HeLa cells

Biochemistry ◽  
1977 ◽  
Vol 16 (12) ◽  
pp. 2783-2791 ◽  
Author(s):  
Stanley F. Wolf ◽  
David Schlessinger
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells

Synthesis of Ribosomal RNA in Synchronized Hela Cells

Nature ◽  
1965 ◽  
Vol 208 (5009) ◽  
pp. 464-466 ◽  
Author(s):  
MATTHEW D. SCHARFF ◽  
ELLIOTT ROBBINS
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells

scholarly journals The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids

1968 ◽  
Vol 107 (6) ◽  
pp. 851-859 ◽  
Author(s):  
M. L. Fenwick
Keyword(s):  
Hydrogen Bonding ◽  
Ribosomal Rna ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Linear Structure ◽  
Sedimentation Rates ◽  
Amino Groups ◽  
Ribonucleic Acids ◽  
Molecular Weights ◽  
Virus Rna

It has been reported that the RNA of several bacteriophages and that of the larger ribosomal sub-units of mammalian cells sediment faster in the presence of 0·1m-sodium chloride than is expected from their estimated molecular weights. The effect of blocking the hydrogen-bonding amino groups of these and other types of RNA was studied. The RNA of phage R17 no longer sedimented anomalously fast after treatment with formaldehyde. In contrast, the larger ribosomal RNA of HeLa cells appeared more aberrant than before, sedimenting faster than tobacco-mosaic-virus RNA (mol.wt. 2×106) in the presence of formaldehyde. The rapidly labelled nuclear 45s RNA of HeLa cells still sedimented faster than the larger ribosomal RNA after reaction with formaldehyde, showing no evidence of disaggregation. It is suggested that both the large ribosomal RNA and the 45s RNA of HeLa cells may have a non-linear structure.

scholarly journals Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable.

1981 ◽  
Vol 1 (6) ◽  
pp. 497-511 ◽  
Author(s):  
R Gelfand ◽  
G Attardi
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
Half Life ◽  
Messenger Rna ◽  
Specific Activity ◽  
Mitochondrial Rna ◽  
Messenger Rnas ◽  
Precursor Pool ◽  
Messenger Ribonucleic Acid

The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes in specific activity of the mitochondrial nucleotide precursor pools. In one experiment, a novel method for determining the nucleotide precursor pool specific activities, using nascent RNA chains, has been utilized. All mitochondrial RNA species analyzed were found to be metabolically unstable, with half-lives of 2.5 to 3.5 h for the two ribosomal RNA components and between 25 and 90 min for the various putative messenger RNAs. A cordycepin "chase" experiment yielded half-life values for the messenger RNA species which were, in general, larger by a factor of 1.5 to 2.5 than those estimated in the labeling kinetics experiments. On the basis of previous observations, a model is proposed whereby the rate of mitochondrial RNA decay is under feedback control by some mechanism linked to RNA synthesis or processing. A short half-life was determined for five large polyadenylated RNAs, which are probably precursors of mature species. A rate of synthesis of one to two molecules per minute per cell was estimated for the various H-strand-coded messenger RNA species, and a rate of synthesis 50 to 100 times higher was estimated for the ribosomal RNA species. These data indicate that the major portion of the H-strand in each mitochondrial deoxyribonucleic acid molecule is transcribed very infrequently, possibly as rarely as once or twice per cell generation. Furthermore, these results are consistent with a previously proposed model of H-strand transcription in the form of a single polycistronic molecule.

Partial sequence analysis of ribosomal RNA from HeLa cells

1968 ◽  
Vol 33 (3) ◽  
pp. 757-775 ◽  
Author(s):  
Philippe Jeanteur ◽  
Francesco Amaldi ◽  
Giuseppe Attardi
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Rna ◽  
Hela Cells ◽  
Partial Sequence

Studies on the Control of Ribosomal RNA Synthesis in HeLa Cells

1975 ◽  
Vol 57 (1) ◽  
pp. 79-83 ◽  
Author(s):  
C. James CHESTERTON ◽  
Barbara E. H. COUPAR ◽  
Peter H. W. BUTTER WORTH ◽  
Janice BUSS ◽  
Melvin H. GREEN
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Rna Synthesis

Methylation of 45 s ribosomal RNA precursor in HeLa cells

1967 ◽  
Vol 23 (2) ◽  
pp. 149-161 ◽  
Author(s):  
Ernest F. Zimmerman ◽  
Barbara W. Holler
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Rna Precursor

scholarly journals THE SELECTIVE INTERRUPTION OF NUCLEOLAR RNA SYNTHESIS IN HELA CELLS BY CORDYCEPIN

1969 ◽  
Vol 41 (2) ◽  
pp. 510-520 ◽  
Author(s):  
Moshe Siev ◽  
Robert Weinberg ◽  
Sheldon Penman
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Rna Synthesis ◽  
Transfer Rna ◽  
Short Exposure ◽  
Low Concentrations ◽  
Dna And Protein Synthesis ◽  
Ribosomal Precursor ◽  
Precursor Molecules ◽  
Nucleolar Rna

Cordycepin is an analogue of adenosine lacking the 3'-OH. When incorporated into a growing RNA molecule, cordycepin prevents further elongation, thus producing a prematurely terminated RNA molecule. When HeLa cells are exposed to low concentrations of cordycepin, DNA and protein synthesis are unaffected during short exposure periods. The synthesis of completed ribosomal and ribosomal-precursor (45S) RNA is significantly depressed. Partially completed 45S ribosomal precursor molecules accumulate in the nucleolus. 18S ribosomal RNA can be cleaved from these incomplete precursors, while 32S ribosomal precursor cannot be produced from partially snythesized 45S molecules. The synthesis of transfer RNA is also reduced in the presence of cordycepin. The synthesis of the nuclear heterogeneous RNA species is unaffected by the drug while the cytoplasmic heterogeneous RNA is slightly reduced.

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