The Effects of Whole-Body X-Irradiation on Mouse Liver Alpha-Hydroxy Acid Oxidase

1969 ◽  
Vol 40 (1) ◽  
pp. 213
Author(s):  
S. Kleinbergs ◽  
I. A. Bernstein
Keyword(s):  
Hydroxy Acid ◽  
Mouse Liver ◽  
Whole Body ◽  
Acid Oxidase ◽  
X Irradiation

THE EFFECT OF 2-AMINOETHYLISOTHIOURONIUM (AET) ON THE RESPIRATION OF MOUSE LIVER AND SPLEEN SLICES

1960 ◽  
Vol 38 (1) ◽  
pp. 819-822
Author(s):  
P. V. Vittorio ◽  
Wilma P. Spence
Keyword(s):  
Mouse Liver ◽  
Whole Body ◽  
Depressing Effect ◽  
Bicarbonate Buffer ◽  
Liver Slices ◽  
X Irradiation

The effect of AET injected in vivo on the respiration of liver and spleen slices from non-irradiated and X-irradiated mice was studied. The mice were killed 4 or 24 hours after the injection of a protective dose of AET and the livers and spleens were removed, sliced, and incubated for 3 hours in Krebs–Henseleit bicarbonate buffer containing uniformly labelled C14 glucose. In both non-irradiated and X-irradiated mice, initially, AET depressed the respiration of C14O2 from incubated liver slices but this depressing effect appeared to be overcome with time. Whole body X irradiation increased the total C14O2 respired by liver slices from mice injected with saline or AET prior to X irradiation and killed 4 or 24 hours after X irradiation. Whole body X irradiation decreased the total C14O2 respired by the spleen slices and the 24-hour samples showed the greatest decrease. In both the non-irradiated and X-irradiated mice AET did not appear to affect the total C14O2 respired by spleen slices.

scholarly journals Oxidation of methionine and 2-hydroxy 4-methylthiobutanoic acid stereoisomers in chicken tissues

1989 ◽  
Vol 62 (1) ◽  
pp. 63-75 ◽  
Author(s):  
Liliane Dupuis ◽  
C. Linda Saunderson ◽  
Antoine Puigserver ◽  
Patrick Brachet
Keyword(s):  
Hydroxy Acid ◽  
Specific Activity ◽  
Whole Body ◽  
Acid Oxidase ◽  
Chicken Tissue ◽  
Tissue Homogenates ◽  
Liver And Kidney ◽  
Oxidation Of Methionine ◽  
Kidney Liver ◽  
Substrate Concentrations

Oxidation of dl-2-hydroxy 4-methylthiobutanoic acid (dl-HMB), dl-methionine (dl-MET) and l-methionine (l-MET) in chicken tissue homogenates was compared using 1-14C-labelled tracers. The pattern of oxidation of the substrates was similar at both low (0.7 mm) and high (20 mm) concentrations. The rate of conversion to 2-keto 4-methylthiobutanoic acid (KMB) was highest for dl-MET and lowest for l-MET in kidney, liver and intestinal mucosa. In breast muscle, rates for dl-MET and l-MET were similar at 0.7 mm, but dl-HMB showed the highest rate at 20 mm. Kidney contained the highest specific activity for oxidation of all three substrates. Raising the pH of liver and kidney homogenates from 7.5 to 8.6 increased the oxidation of dl-MET, exclusively. Experiments with inhibitors of D-2-hydroxy acid dehydrogenase (EC 1.1.99.6) and L-2-hydroxy acid oxidase (EC 1.1.3.15) suggested that d- and l-HMB were stereospecifically oxidized by the enzymes. KMB stimulated l-MET oxidation in kidney yet inhibited l-MET oxidation in liver homogenates. The effect of KMB on dl-MET and dl-HMB oxidation also varied between tissues. Amino-oxyacetate inhibited l-MET oxidation completely and dl-MET and dl-HMB oxidation almost completely in both kidney and liver. L-Cycloserine was less potent than amino-oxyacetate and decreased l-MET oxidation more in kidney than in liver. It can be calculated from the results that, at low substrate concentrations, the liver contributes principally to the whole body oxidation of both dl-HMB and dl-MET. At high (greater than physiological) concentrations, dl-HMB would be oxidized principally in skeletal muscle. At all concentrations, l-MET would be converted to KMB mainly in the muscle.

THE EFFECT OF 2-AMINOETHYLISOTHIOURONIUM (AET) ON THE RESPIRATION OF MOUSE LIVER AND SPLEEN SLICES

1960 ◽  
Vol 38 (8) ◽  
pp. 819-822
Author(s):  
P. V. Vittorio ◽  
Wilma P. Spence
Keyword(s):  
Mouse Liver ◽  
Whole Body ◽  
Depressing Effect ◽  
Bicarbonate Buffer ◽  
Liver Slices ◽  
X Irradiation

The effect of AET injected in vivo on the respiration of liver and spleen slices from non-irradiated and X-irradiated mice was studied. The mice were killed 4 or 24 hours after the injection of a protective dose of AET and the livers and spleens were removed, sliced, and incubated for 3 hours in Krebs–Henseleit bicarbonate buffer containing uniformly labelled C14 glucose. In both non-irradiated and X-irradiated mice, initially, AET depressed the respiration of C14O2 from incubated liver slices but this depressing effect appeared to be overcome with time. Whole body X irradiation increased the total C14O2 respired by liver slices from mice injected with saline or AET prior to X irradiation and killed 4 or 24 hours after X irradiation. Whole body X irradiation decreased the total C14O2 respired by the spleen slices and the 24-hour samples showed the greatest decrease. In both the non-irradiated and X-irradiated mice AET did not appear to affect the total C14O2 respired by spleen slices.

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