The Indirect Effect of Irradiation on Embryonic Development: III. The Contribution of Ovarian Irradiation, Uterine Irradiation, Oviduct Irradiation, and Zygote Irradiation to Fetal Mortality and Growth Retardation in the Rat

1967 ◽  
Vol 30 (4) ◽  
pp. 759 ◽  
Author(s):  
Robert L. Brent ◽  
Booker T. Bolden
Keyword(s):  
Embryonic Development ◽  
Growth Retardation ◽  
Indirect Effect ◽  
Fetal Mortality ◽  
Ovarian Irradiation

Effects of position in the uterus on fetal mortality and on response to trypan blue

Development ◽  
1967 ◽  
Vol 17 (3) ◽  
pp. 617-624
Author(s):  
Sidney L. Beck
Keyword(s):  
Embryonic Development ◽  
Blood Supply ◽  
Relative Position ◽  
Trypan Blue ◽  
Normal Development ◽  
Uterine Horn ◽  
Fetal Mortality ◽  
Uterine Environment ◽  
The Common

In a polytocous species such as the mouse, the relative position in the uterus that a fetus occupies may well have an effect upon the likelihood of normal development or indeed survival (Trasler, 1960; Woollam & Millen, 1962). It would seem desirable, therefore, especially since the mouse is so valuable a tool in studies of development (Grüneberg, 1963) and teratogenesis (Fave, 1964; Woollam, 1966), to learn as much as possible about the contribution of the uterine environment to embryonic development. It has been suggested that fetuses implanted in the portion of the uterine horn nearest the common uterus may have an increased tendency toward death or deformity when their dams are made anoxic experimentally during gestation (Woollam & Millen, 1962). These authors suggest that the variations in response to anoxia might be accounted for by differences in blood supply or different times of implantation in the different regions of the uterus.

scholarly journals Loss of Ing3 Expression Results in Growth Retardation and Embryonic Death

Cancers ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 80
Author(s):  
Dieter Fink ◽  
Tienyin Yau ◽  
Arash Nabbi ◽  
Bettina Wagner ◽  
Christine Wagner ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Cancer Progression ◽  
Growth Retardation ◽  
Developmental Disorders ◽  
Tumour Suppressor ◽  
Neural Tube Closure ◽  
Developmental Defect ◽  
Histone Acetyl Transferase ◽  
Transgenic Mouse Strain ◽  
Candidate Tumour Suppressor

The ING3 candidate tumour suppressor belongs to a family of histone modifying proteins involved in regulating cell proliferation, senescence, apoptosis, chromatin remodeling, and DNA repair. It is a stoichiometric member of the minimal NuA4 histone acetyl transferase (HAT) complex consisting of EAF6, EPC1, ING3, and TIP60. This complex is responsible for the transcription of an essential cascade of genes involved in embryonic development and in tumour suppression. ING3 has been linked to head and neck and hepatocellular cancers, although its status as a tumour suppressor has not been well established. Recent studies suggest a pro-metastasis role in prostate cancer progression. Here, we describe a transgenic mouse strain with insertional mutation of an UbC-mCherry expression cassette into the endogenous Ing3 locus, resulting in the disruption of ING3 protein expression. Homozygous mutants are embryonically lethal, display growth retardation, and severe developmental disorders. At embryonic day (E) 10.5, the last time point viable homozygous embryos were found, they were approximately half the size of heterozygous mice that develop normally. µCT analysis revealed a developmental defect in neural tube closure, resulting in the failure of formation of closed primary brain vesicles in homozygous mid-gestation embryos. This is consistent with high ING3 expression levels in the embryonic brains of heterozygous and wild type mice and its lack in homozygous mutant embryos that show a lack of ectodermal differentiation. Our data provide direct evidence that ING3 is an essential factor for normal embryonic development and that it plays a fundamental role in prenatal brain formation.

The effect of cathepsin inhibitor on rat embryos grown in vitro

Development ◽  
1982 ◽  
Vol 71 (1) ◽  
pp. 1-9
Author(s):  
Felix Beck ◽  
Adam Lowy
Keyword(s):  
Amino Acids ◽  
Embryonic Development ◽  
Growth Retardation ◽  
Culture Medium ◽  
Storage Disease ◽  
Morphological Changes ◽  
Protein Breakdown ◽  
Rat Embryos ◽  
Visceral Layer

The addition of leupeptin to New cultures of rat embryos produces growth retardation and abnormalities of embryonic development. The effect is probably due to inhibition of the maternal protein breakdown necessary for embryonic growth. This function is carried out by the visceral layer of the yolk sac which shows distinct morphological changes akin to storage disease when leupeptin is added to the culture medium. We have not found it possible to reverse the effects of leupeptin by addition of amino acids to the culture medium.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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