scholarly journals Influence of Exogenous Estrogen Receptor Ligands on Uterine Leiomyoma: Evidence from an in Vitro/in Vivo Animal Model for Uterine Fibroids

2000 ◽  
Vol 108 ◽  
pp. 829 ◽  
Author(s):  
Deborah S. Hunter ◽  
Leslie C. Hodges ◽  
Patricia K. Eagon ◽  
Peter M. Vonier ◽  
Robin Fuchs-Young ◽  
...  
Keyword(s):  
Animal Model ◽  
Estrogen Receptor ◽  
Uterine Leiomyoma ◽  
Uterine Fibroids ◽  
Receptor Ligands ◽  
Exogenous Estrogen ◽  
In Vivo Animal Model ◽  
Estrogen Receptor Ligands

scholarly journals Influence of Exogenous Estrogen Receptor Ligands on Uterine Leiomyoma: Evidence from an in Vitro/in Vivo Animal Model for Uterine Fibroids

2000 ◽  
Vol 108 (s5) ◽  
pp. 829-834 ◽  
Author(s):  
Deborah S Hunter ◽  
Leslie C Hodges ◽  
Patricia K Eagon ◽  
Peter M Vonier ◽  
Robin Fuchs-Young ◽  
...  
Keyword(s):  
Animal Model ◽  
Estrogen Receptor ◽  
Uterine Leiomyoma ◽  
Uterine Fibroids ◽  
Receptor Ligands ◽  
Exogenous Estrogen ◽  
In Vivo Animal Model ◽  
Estrogen Receptor Ligands

scholarly journals Interpreting TMEM67 missense variants of uncertain significance (VUS) in an animal model

2021 ◽  
Author(s):  
Karen I Lange ◽  
Sunayna Best ◽  
Sofia Tsiropoulou ◽  
Ian Berry ◽  
Colin A Johnson ◽  
...  
Keyword(s):  
Animal Model ◽  
Molecular Genetic ◽  
Genetic Diagnosis ◽  
Knock Out ◽  
Variants Of Uncertain Significance ◽  
C Elegans ◽  
In Vivo Animal Model ◽  
Uncertain Significance

Purpose: A molecular genetic diagnosis is essential for accurate counselling and management of patients with ciliopathies. Uncharacterized missense alleles are often classified as variants of uncertain significance (VUS) and are not clinically useful. In this study, we explore the use of a tractable animal model (C. elegans) for in vivo interpretation of missense VUS alleles of TMEM67, a gene frequently mutated as a cause of ciliopathies. Methods: CRISPR/Cas9 gene editing was used to generate homozygous worm strains carrying TMEM67 patient variants. Quantitative phenotypic assays (dye filling, roaming, chemotaxis) assessed cilia structure and function. Results were validated by genetic complementation assays in a human TMEM67 knock-out hTERT-RPE1 cell line. Results: Quantitative assays in C. elegans distinguished between known benign (Asp359Glu, Thr360Ala) and pathogenic (Glu361Ter, Gln376Pro) variants. Analysis of seven missense VUS alleles predicted two benign (Cys173Arg, Thr176Ile) and four pathogenic variants (Cys170Tyr, His782Arg, Gly786Glu, His790Arg). Results from one VUS (Gly979Arg) were inconclusive in worms, but additional in vitro validation suggested it was likely benign. Conclusion: Efficient genome editing and quantitative functional assays in C. elegans make it a tractable in vivo animal model that allows stratification and rapid, cost-effective interpretation of ciliopathy-associated missense VUS alleles.

Thermo-responsive chitosan hydrogel for healing of full-thickness wounds infected with XDR bacteria isolated from burn patients: In vitro and in vivo animal model

2020 ◽  
Vol 164 ◽  
pp. 4475-4486
Author(s):  
Zahra Aliakbar Ahovan ◽  
Sadjad Khosravimelal ◽  
Behnaz Sadat Eftekhari ◽  
Soraya Mehrabi ◽  
Ali Hashemi ◽  
...  
Keyword(s):  
Animal Model ◽  
Burn Patients ◽  
Full Thickness ◽  
Chitosan Hydrogel ◽  
In Vivo Animal Model

In vitro activity and in vivo animal model efficacy of IB-367 alone and in combination with imipenem and colistin against Gram-negative bacteria

Peptides ◽  
2014 ◽  
Vol 55 ◽  
pp. 17-22 ◽  
Author(s):  
Oriana Simonetti ◽  
Oscar Cirioni ◽  
Roberto Ghiselli ◽  
Fiorenza Orlando ◽  
Carmela Silvestri ◽  
...  
Keyword(s):  
Animal Model ◽  
Vitro Activity ◽  
Gram Negative Bacteria ◽  
In Vitro Activity ◽  
Gram Negative ◽  
In Vivo Animal Model

scholarly journals Amalgamation and Characterization of Copper Nanoparticles Alleviated With Stachytarpheta Cayennensis and Its Anti-cancer Activity in Both in Vitro and in Vivo Animal Model

2021 ◽  
Author(s):  
Vidya Devanathadesikan Seshadri
Keyword(s):  
Animal Model ◽  
Skin Cancer ◽  
Copper Nanoparticles ◽  
Enzymatic Antioxidants ◽  
In Vivo Animal Model ◽  
Anti Cancer ◽  
Stachytarpheta Cayennensis ◽  
Cancer Activity

Abstract Melanoma is an extremely malignant skin cancer with a probability of metastasis and accountable for the mainstream skin associated mortality. In the present study, we described the novel usage of Stachytarpheta cayennensis mediated copper nanoparticles and its anti-cancer activity in both in vitro and in vivo model of skin cancer. The synthesis of Cu-NPs was confirmed using UV-absorbance peak values ranging from 325-345 nm. The size of the nanoparticles was around 90nm, as deduced by the dynamic light scattering study.Furthermore, transmission electron microscopy (TEM) established that the morphology of the copper particles. Cytotoxicity of Cu-NPs of Stachytarpheta cayennensis illustrates the toxicity level of Stachytarpheta cayennensis. Also, the anti-cancer potential of Cu-NPs was evaluated in A375 cells. In experimental animals, body biochemical parameters like SOD, CAT, GSH were diminished in DMBA induced animals while Cu-NPs treatment raised the levels of the aforementioned enzymatic antioxidants compared to the control animals. Additionally, cytotoxicity assay, mitochondrial membrane potential (MMP), cell adhesion analysis, and the estimation of reactive oxygen species (ROS) in the presence of Cu-NPs was evaluated by standard protocols. The present study's outcomes confirm the defensive and valuable effects of copper loaded Stachytarpheta cayennensis against DMBA induced skin melanoma, animal model.

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