In vitro anti-inflammatory properties of fermented pepino (Solanum muricatum ) milk by γ -aminobutyric acid-producing Lactobacillus brevis and an in vivo animal model for evaluating its effects on hypertension

2015 ◽  
Vol 96 (1) ◽  
pp. 192-198 ◽  
Author(s):  
Vincent Hung-Shu Chang ◽  
Tsai-Hsin Chiu ◽  
Szu-Chieh Fu
Keyword(s):  
Animal Model ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Solanum Muricatum ◽  
In Vivo Animal Model ◽  
Anti Inflammatory

scholarly journals Interpreting TMEM67 missense variants of uncertain significance (VUS) in an animal model

2021 ◽  
Author(s):  
Karen I Lange ◽  
Sunayna Best ◽  
Sofia Tsiropoulou ◽  
Ian Berry ◽  
Colin A Johnson ◽  
...  
Keyword(s):  
Animal Model ◽  
Molecular Genetic ◽  
Genetic Diagnosis ◽  
Knock Out ◽  
Variants Of Uncertain Significance ◽  
C Elegans ◽  
In Vivo Animal Model ◽  
Uncertain Significance

Purpose: A molecular genetic diagnosis is essential for accurate counselling and management of patients with ciliopathies. Uncharacterized missense alleles are often classified as variants of uncertain significance (VUS) and are not clinically useful. In this study, we explore the use of a tractable animal model (C. elegans) for in vivo interpretation of missense VUS alleles of TMEM67, a gene frequently mutated as a cause of ciliopathies. Methods: CRISPR/Cas9 gene editing was used to generate homozygous worm strains carrying TMEM67 patient variants. Quantitative phenotypic assays (dye filling, roaming, chemotaxis) assessed cilia structure and function. Results were validated by genetic complementation assays in a human TMEM67 knock-out hTERT-RPE1 cell line. Results: Quantitative assays in C. elegans distinguished between known benign (Asp359Glu, Thr360Ala) and pathogenic (Glu361Ter, Gln376Pro) variants. Analysis of seven missense VUS alleles predicted two benign (Cys173Arg, Thr176Ile) and four pathogenic variants (Cys170Tyr, His782Arg, Gly786Glu, His790Arg). Results from one VUS (Gly979Arg) were inconclusive in worms, but additional in vitro validation suggested it was likely benign. Conclusion: Efficient genome editing and quantitative functional assays in C. elegans make it a tractable in vivo animal model that allows stratification and rapid, cost-effective interpretation of ciliopathy-associated missense VUS alleles.

Thermo-responsive chitosan hydrogel for healing of full-thickness wounds infected with XDR bacteria isolated from burn patients: In vitro and in vivo animal model

2020 ◽  
Vol 164 ◽  
pp. 4475-4486
Author(s):  
Zahra Aliakbar Ahovan ◽  
Sadjad Khosravimelal ◽  
Behnaz Sadat Eftekhari ◽  
Soraya Mehrabi ◽  
Ali Hashemi ◽  
...  
Keyword(s):  
Animal Model ◽  
Burn Patients ◽  
Full Thickness ◽  
Chitosan Hydrogel ◽  
In Vivo Animal Model

In vitro activity and in vivo animal model efficacy of IB-367 alone and in combination with imipenem and colistin against Gram-negative bacteria

Peptides ◽  
2014 ◽  
Vol 55 ◽  
pp. 17-22 ◽  
Author(s):  
Oriana Simonetti ◽  
Oscar Cirioni ◽  
Roberto Ghiselli ◽  
Fiorenza Orlando ◽  
Carmela Silvestri ◽  
...  
Keyword(s):  
Animal Model ◽  
Vitro Activity ◽  
Gram Negative Bacteria ◽  
In Vitro Activity ◽  
Gram Negative ◽  
In Vivo Animal Model

scholarly journals Influence of Exogenous Estrogen Receptor Ligands on Uterine Leiomyoma: Evidence from an in Vitro/in Vivo Animal Model for Uterine Fibroids

2000 ◽  
Vol 108 ◽  
pp. 829 ◽  
Author(s):  
Deborah S. Hunter ◽  
Leslie C. Hodges ◽  
Patricia K. Eagon ◽  
Peter M. Vonier ◽  
Robin Fuchs-Young ◽  
...  
Keyword(s):  
Animal Model ◽  
Estrogen Receptor ◽  
Uterine Leiomyoma ◽  
Uterine Fibroids ◽  
Receptor Ligands ◽  
Exogenous Estrogen ◽  
In Vivo Animal Model ◽  
Estrogen Receptor Ligands

scholarly journals Immunomodulatory Properties of a γ-Aminobutyric Acid-Enriched Strawberry Juice Produced by Levilactobacillus brevis CRL 2013

2020 ◽  
Vol 11 ◽  
Author(s):  
Pablo G. Cataldo ◽  
Julio Villena ◽  
Mariano Elean ◽  
Graciela Savoy de Giori ◽  
Lucila Saavedra ◽  
...  
Keyword(s):  
Current Trend ◽  
Starter Culture ◽  
Aminobutyric Acid ◽  
Inflammatory Factors ◽  
Food Ingredient ◽  
Protein Amino Acid ◽  
Anti Inflammatory ◽  
Strawberry Juice

Gamma-aminobutyric acid (GABA) plays a key role in mammals as the major inhibitory neurotransmitter of the central nervous system. Although GABA may not be able to cross the human blood-brain barrier, it was approved as a food ingredient because of its benefits to the host after oral administration including anti-hypertensive, anti-depressant and anti-inflammatory activities. Considering the current trend toward the development of new functional and natural products and that microbial fermentation is one of the most promising methods to produce this non-protein amino acid, the in situ production of GABA through fermentation of strawberry and blueberry juices by the efficient GABA producer strain, Levilactobacillus brevis (formerly known as Lactobacillus brevis) CRL 2013, was evaluated. A high GABA production (262 mM GABA) was obtained after fermenting strawberry juice supplemented with yeast extract for 168 h, being GABA yield significantly higher in strawberry juices than in the blueberry ones. Thus, GABA-enriched fermented strawberry juice (FSJ) was selected to carry out in vivo and in vitro studies. The in vitro functional analysis of the GABA-enriched FSJ demonstrated its ability to significantly decrease the expression of cox-2 gene in LPS stimulated RAW 264.7 macrophages. In addition, in vivo studies in mice demonstrated that both, L. brevis CRL 2013 and the GABA-enriched FSJ were capable of reducing the levels of peritoneal, intestinal and serum TNF-α, IL-6, and CXCL1, and increasing IL-10 and IFN-γ in mice exposed to an intraperitoneal challenge of LPS. Of note, the GABA-enriched FSJ was more efficient than the CRL 2013 strain to reduce the pro-inflammatory factors and enhance IL-10 production. These results indicated that the CRL 2013 strain exerts anti-inflammatory effects in the context of LPS stimulation and that this effect is potentiated by fermentation. Our results support the potential use of L. brevis CRL 2013 as an immunomodulatory starter culture and strawberry juice as a remarkable vegetable matrix for the manufacture of GABA-enriched fermented functional foods capable of differentially modulating the inflammatory response triggered by TLR4 activation.

scholarly journals DEVELOPMENT AND CHARACTERIZATION OF BIOCOMPATIBLE POLYHYDROXY BUTYRATE IMPREGNATED WITH HERBAL PLANTS AGAINST WOUND HEALING ACTIVITY ON IN VIVO ANIMAL MODEL

2019 ◽  
pp. 196-201
Author(s):  
RAM NARENDRAN R ◽  
MALEEKA BEGUM SF ◽  
RUBAVATHI S
Keyword(s):  
Wound Healing ◽  
Drug Carrier ◽  
In Vitro Cytotoxicity ◽  
Toxic Substances ◽  
Cast Film ◽  
In Vivo Animal Model ◽  
Anti Inflammatory ◽  
Wound Healing Activity

Objective: The current study is to evaluate the antimicrobial, antioxidant, anti-inflammatory, and in vitro cytotoxicity activities of polyhydroxybutyrate (PHB) and to develop the herbal impregnated PHB cast film for wound healing activities using Albino Wistar rat model. Methods: PHB produced by Azotobacter chroococcum A3 strain was synthesized and characterized (previous study). The PHB was subjected to various biocompatibility studies such as antimicrobial, antioxidant, and anti-inflammatory studies. The PHB was also subjected to cytotoxicity study by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. PHB films were made using different combinations of plant and algal blends (herbal blends). The herbal blends of PHB films were evaluated for in vivo wound healing activity using Albino Wistar rats. Results: The turmeric impregnated PHB showed the highest result for antimicrobial with 27.25±0.23 mm against skin pathogens and antioxidant activity with the highest percentage of inhibition of 76%. The result predicts that PHB will not let to any toxic substances rather it acts as a chemoprotective agent followed by the inhibitory concentration value was found to be 1.56 μg/ml for 100 μg. The in vivo study showed better wound healing activity for PHB blended with 2% turmeric leaf and rhizome cast film. Whereas the wound healing activity of control and crude PHB was 90.4±0.4 and 91.3±0.56 respectively. Conclusion: The results from the present study showed that PHB can act as a good candidate for drug carrier and it is biocompatible in living cells.

scholarly journals Influence of Exogenous Estrogen Receptor Ligands on Uterine Leiomyoma: Evidence from an in Vitro/in Vivo Animal Model for Uterine Fibroids

2000 ◽  
Vol 108 (s5) ◽  
pp. 829-834 ◽  
Author(s):  
Deborah S Hunter ◽  
Leslie C Hodges ◽  
Patricia K Eagon ◽  
Peter M Vonier ◽  
Robin Fuchs-Young ◽  
...  
Keyword(s):  
Animal Model ◽  
Estrogen Receptor ◽  
Uterine Leiomyoma ◽  
Uterine Fibroids ◽  
Receptor Ligands ◽  
Exogenous Estrogen ◽  
In Vivo Animal Model ◽  
Estrogen Receptor Ligands
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