Macrophage Function in Patients with American Cutaneous Leishmaniasis: In vitro Cytotoxicity and Interleukin-1 Production

1987 ◽  
Vol 73 (4) ◽  
pp. 769 ◽  
Author(s):  
P. -R. Ridel ◽  
J. -P. Dedet ◽  
P. Esterre
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Interleukin 1 ◽  
In Vitro Cytotoxicity ◽  
Macrophage Function ◽  
American Cutaneous Leishmaniasis

In vitro infectivity of species of Leishmania (Viannia) responsible for American cutaneous leishmaniasis

2008 ◽  
Vol 103 (4) ◽  
pp. 771-776 ◽  
Author(s):  
Marliane Batista Campos ◽  
Cláudia Maria De Castro Gomes ◽  
Adelson Alcimar Almeida de Souza ◽  
Ralph Lainson ◽  
Carlos Eduardo Pereira Corbett ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
American Cutaneous Leishmaniasis

American Leishmania spp: Formycin B treatment of cutaneous leishmaniasis in mice

Parasitology ◽  
1987 ◽  
Vol 94 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Tamara Rojas ◽  
J. L. Avila
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Optimal Dose ◽  
Dose Schedule ◽  
Inhibitory Effect ◽  
American Cutaneous Leishmaniasis ◽  
Leishmania Spp ◽  
Low Toxicity ◽  
Foot Pad

Using foot-pad infection of female C57BL/6, DBA/2J and NMRI-IVIC mice as an animal model for American cutaneous leishmaniasis (ACL), we evaluated the inhibitory effect of Formycin B (FoB) on the infection produced by 7 different Leishmania isolates. When treatment was initiated some days, or even some weeks, after infection a significant leishmanistatic effect was detected on mice infected with all Leishmania isolates, which reached 30–55 weeks for some isolates. The optimal dose schedule was 1·25 mg/kg body weight/day, injected intraperitoneally for 20 consecutive days. Significant differences in the sensitivity of various Leishmania spp. to FoB were found, either in vivo, or in vitro where a high [3H]FoB incorporation rate was found only for certain Leishmania isolates. The low toxicity of this drug and the sensitivity of the 7 Leishmania isolates tested suggest that FoB could be useful in the treatment of ACL.

scholarly journals PARTICIPAÇÃO DOS RECEPTORES TOLL-LIKE NA RESPOSTA A INFECÇÃO in vitro POR Leishmania (Viannia) braziliensis: UMA REVISÃO DE LITERATURA

2021 ◽  
Vol 2 (1) ◽  
pp. 54
Author(s):  
Anna Clara Silva Fonseca ◽  
Nathália Brígida de Oliveira ◽  
Daniela Camargos Costa
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Natural Killer ◽  
Leishmania Braziliensis ◽  
Toll Like Receptors ◽  
American Cutaneous Leishmaniasis ◽  
Tnf Α ◽  
Mucosal Leishmaniasis

Introdução: A Leishmania braziliensis é um protozoário da família Trypanosomatidae responsável pelo desenvolvimento de uma das formas dermotrópicas da doença denominada leishmaniose mucosa (LM). O parasito é intracelular obrigatório e se multiplica dentro dos macrófagos e monócitos de seus hospedeiros. Os receptores tolllike (TLR) são glicoproteínas transmembrana presentes nas células de defesa, principalmente Natural Killer, macrófagos e células dendríticas, que reconhecem estruturas microbianas e que promovem uma série de sinais que produzem citocinas próinflamatórias importantes para que a resposta imune inata seja efetiva, como por exemplo TNF-α, IL-10 e TGF-β. Objetivo: Realizar revisão da literatura a respeito do papel dos receptores Toll-like na resposta à infecção in vitro por L. braziliensis. Material e métodos: Realizado no formato de revisão integrativa, a pesquisa abrangeu artigos científicos das bases de dados SCIELO e PUBMED. Os descritores utilizados foram “American Cutaneous leishmaniasis”, “Leishmania braziliensis”, “mucosal leishmaniasis” e “Toll-like receptors”. Foram selecionados artigos acadêmicos originais, escritos na língua inglesa, que foram publicados no período de 2014 a 2020, obtendo -se 20 artigos. Após a leitura dos títulos e resumos e ao adotar os critérios de exclusão, foram selecionados 12 artigos originais para a presente revisão. Resultados: As pesquisas selecionadas avaliaram 147 pacientes com diagnóstico histopatológico de Leishmaniose Cutânea (LC). Os pacientes que apresentavam LM, concomitantemente possuíam uma maior expressão de células TCD4+, TCD8+, IL-10+ e TGF-β+ em comparação com aqueles que possuíam LC, que expressavam uma menor quantidades dessas citocinas. Em oposição, o TNF-α em pacientes com LC, encontrava-se aumentado se comparada a LM. Na análise relacionada a TLR-2 e TLR-4, houve uma maior expressão em monócitos nos pacientes com LC associadas a L. braziliensis e que o bloqueio dos TRL reduziu as respostas oxidativas das células de pacientes com LC. Conclusão: Diante dos resultados é possível concluir que a maior expressão de TLR-2 e TLR-4 promove a hiper-reatividade de citocinas pró-inflamatórias e consequente pré-disposição para desenvolvimento da doença. Portanto, seu bloqueio é eficaz para redução das lesões. Esse dado demonstra a relação e o papel dos TLR na infecção por L. braziliensis, trazendo importantes esclarecimentos para a resposta imune e desenho vacinal.

scholarly journals American cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis resistant to meglumine antimoniate, but with good response to pentamidine: a case report

2011 ◽  
Vol 44 (2) ◽  
pp. 254-256 ◽  
Author(s):  
Maria Inês Fernandes Pimentel ◽  
Cibele Baptista ◽  
Évelyn Figueiredo Rubin ◽  
Érica de Camargo Ferreira e Vasconcellos ◽  
Marcelo Rosandiski Lyra ◽  
...  
Keyword(s):  
Case Report ◽  
Cutaneous Leishmaniasis ◽  
Amphotericin B ◽  
Meglumine Antimoniate ◽  
American Cutaneous Leishmaniasis ◽  
Systemic Treatments ◽  
Poor Tolerance

This is a case report of a Brazilian soldier with cutaneous leishmaniasis. The lesion relapsed following two systemic treatments with meglumine antimoniate. The patient was treated with amphotericin B, which was interrupted due to poor tolerance. Following isolation of Leishmania sp., six intralesional infiltrations of meglumine antimoniate resulted in no response. Leishmania sp promastigotes were again isolated. The patient was submitted to intramuscular 4mg/kg pentamidine. Parasites from the first and second biopsies were identified as Leishmania (Viannia) braziliensis; those isolated from the first biopsy were more sensitive to meglumine antimoniate in vitro than those isolated from the second biopsy. No relapse was observed.

scholarly journals Immune responses induced by a Leishmania (Leishmania) amazonensis recombinant antigen in mice and lymphocytes from vaccinated subjects

1997 ◽  
Vol 39 (2) ◽  
pp. 71-78 ◽  
Author(s):  
Ana Paula FERNANDES ◽  
Elizabeth Cortez HERRERA ◽  
Wilson MAYRINK ◽  
Ricardo T. GAZZINELLI ◽  
Wen Yu LIU ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Recombinant Antigen ◽  
Leishmania Amazonensis ◽  
Mouse Strains ◽  
Susceptible Mouse ◽  
Blood Leukocytes ◽  
Th1 Cell ◽  
American Cutaneous Leishmaniasis ◽  
Antigenic Properties

In the search for Leishmania recombinant antigens that can be used as a vaccine against American Cutaneous Leishmaniasis, we identified a Leishmania (Leishmania) amazonensis recombinant protein of 33 kD (Larp33) which is recognized by antibodies and peripheral blood leukocytes (PBL) from subjects vaccinated with Leishvacin ®, Larp33 was expressed in Escherichia coli after cloning of a 2,2 kb Sau3A digested genomic fragment of L. (L.) amazonensis into the pDS56-6 His vector. Immunoblotting analysis indicated that Larp33 corresponds to an approximately 40-kD native protein expressed in promastigotes of L.(L.) amazonensis and L. (Viannia) braziliensis. Northern blots of total RNA also demonstrated that the gene coding for this protein is expressed in promastigotes of the major lineages of Leishmania causing American Cutaneous Leishmaniasis. Larp33 induced partial protection in susceptible mouse strains (BALB/c and C57BL/10) against L. (L.) amazonensis after vaccination using Bacille Calmette-Guerin (BCG) as adjuvant. In vitro stimulation of splenocytes from BALB/c protected mice with Larp33 elicited the secretion of IL-2 and IFN-<FONT FACE="Symbol">g</font>, suggesting that a Th1 cell-mediated protective response is associated with the resistance observed in these mice. As revealed by its immunogenic and antigenic properties, this novel recombinant antigen is a suitable candidate to compose a vaccine against cutaneous leishmaniasis

In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests

2018 ◽  
Vol 21 (4) ◽  
pp. 262-270 ◽  
Author(s):  
Zehao Huang ◽  
Na Li ◽  
Kaifeng Rao ◽  
Cuiting Liu ◽  
Zijian Wang ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
A549 Cells ◽  
Quantitative Structure Activity Relationship ◽  
In Vitro Cytotoxicity ◽  
Cytotoxic Effects ◽  
Qsar Analysis ◽  
Cell Assays ◽  
Micronucleus Tests ◽  
Damaging Effects

Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations. Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China. Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549. Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.

Mixed Micelles as Nano Polymer Therapeutics of Docetaxel: Increased In vitro Cytotoxicity and Decreased In vivo Toxicity

2018 ◽  
Vol 15 (4) ◽  
pp. 564-575 ◽  
Author(s):  
Arehalli S. Manjappa ◽  
Popat S. Kumbhar ◽  
Prajakta S. Khopade ◽  
Ajit B. Patil ◽  
John I. Disouza
Keyword(s):  
Mixed Micelles ◽  
In Vitro Cytotoxicity ◽  
Polymer Therapeutics ◽  
In Vivo Toxicity
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