In vitro Cultivation of the Digenetic Trematode Sphaeridiotrema globulus (Rudolphi) from the Metacercarial Stage to Egg Production

1969 ◽  
Vol 55 (1) ◽  
pp. 136 ◽  
Author(s):  
Allen K. Berntzen ◽  
Ralph W. Macy
Keyword(s):  
Egg Production ◽  
Digenetic Trematode ◽  
In Vitro Cultivation

scholarly journals Oviposition by Schistosoma mansoni during in vitro cultivation

1996 ◽  
Vol 38 (6) ◽  
pp. 423-426 ◽  
Author(s):  
Leo Roberto Barth ◽  
Ana Paula Morais Fernandes ◽  
Vanderlei Rodrigues
Keyword(s):  
Schistosoma Mansoni ◽  
Egg Production ◽  
Culture Medium ◽  
Parasite Development ◽  
In Vitro Cultivation ◽  
Progressive Reduction ◽  
Maximum Period ◽  
Medium Time ◽  
Kinetics Of

Observation of Schistosoma mansoni oviposition during in vitro culture of adult worms for a maximum period of 10 days showed three well distinct phases in the kinetics of oviposition: an initial phase with low egg production, a period of maximum oviposition and finally a progressive reduction in the number of eggs during the late phases of culture. The kinetics of oviposition and the number of eggs laid by the parasites are influenced by the number of worm pairs per amount of RPMI 1640 medium, time of parasite development in the vertebrate host and type of serum utilized in the culture medium.

Preliminary experiments on the in vitro cultivation of an amphistome Orthocoelium scoliocoelium (Trematoda: Digenea)

1982 ◽  
Vol 56 (3) ◽  
pp. 169-176 ◽  
Author(s):  
A. N. Sharma ◽  
P. N. Sharma
Keyword(s):  
Amino Acids ◽  
Egg Production ◽  
Rumen Fluid ◽  
Inorganic Salts ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Physiological Alterations

ABSTRACTA medium containing inorganic salts, vitamins and amino-acids is described for the in vitro cultivation of the amphistome, Orthocoelium scoliocoelium, from the rumen of buffalo. Based on the properties of rumen fluid, this medium promoted and sustained normal egg production, at 37°C temperature and pH 7·4 to 7·8 for 15 days. In contrast to other media used, no patho-physiological alterations except abnormal cytoplasmic lipid, were detected. Since the parasites produced normal eggs, it is concluded that this medium is suitable for short-term cultivation of the parasites.

scholarly journals Optimization of Conditions for In Vitro Culture of the Microphallid DigeneanGynaecotyla adunca

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Jenna West ◽  
Alexandra Mitchell ◽  
Oscar J. Pung
Keyword(s):  
Egg Production ◽  
Horse Serum ◽  
Calf Serum ◽  
Culture Conditions ◽  
In Vitro Cultivation ◽  
Ilyanassa Obsoleta ◽  
Uca Pugnax ◽  
Newborn Calf Serum ◽  
Chicken Serum

In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode,Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs,Uca pugnax, excysted in trypsin, were incubated overnight to permit fertilization, and were cultured in different conditions to find those that resulted in maximum worm longevity and egg production. When cultured in media lacking serum, worms lived longer in Hanks balanced salt solution and Dulbecco’s Modified Eagle medium/F-12 (DME/F-12) than in RPMI-1640 but produced the most eggs in DME/F-12. Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum. Horse serum was chosen over chicken serum due to the formation of a precipitate in chicken serum. The optimal concentration of horse serum with respect to egg production ranged from 5 to 20%. Infectivity of eggs deposited by worms in culture was tested by feeding eggs to mud snails,Ilyanassa obsoleta. None of these snails producedG. aduncacercariae.

The in vitro cultivation of Hymenolepis microstoma from cysticercoid to egg-producing adult

1970 ◽  
Vol 48 (5) ◽  
pp. 1135-1137 ◽  
Author(s):  
W. S. Evans
Keyword(s):  
Growth And Development ◽  
Egg Production ◽  
Liver Extract ◽  
Horse Serum ◽  
Body Growth ◽  
In Vitro Cultivation ◽  
General Body ◽  
Hymenolepis Microstoma

Cysticercoids of Hymenolepis microstoma have been cultured in vitro to egg-producing adults in 16 days using Eagle's medium with horse serum plus sheep or hamster liver extract, and ox bile. Bile was essential for egg production but not for general body growth. Worms became gravid when cultured under 0, 5, and 10% oxygen. However, growth and development was greatly inhibited when higher concentrations of oxygen were used.

scholarly journals High-resolution AP-SMALDI MSI as a tool for drug imaging in Schistosoma mansoni

2021 ◽  
Author(s):  
Annika S. Mokosch ◽  
Stefanie Gerbig ◽  
Christoph G. Grevelding ◽  
Simone Haeberlein ◽  
Bernhard Spengler
Keyword(s):  
Schistosoma Mansoni ◽  
Egg Production ◽  
Drug Repurposing ◽  
Cancer Drug ◽  
Ionization Mass ◽  
Parasitic Flatworm ◽  
Paired Female ◽  
First Time ◽  
Organ Tropism

AbstractSchistosoma mansoni is a parasitic flatworm causing schistosomiasis, an infectious disease affecting several hundred million people worldwide. Schistosomes live dioeciously, and upon pairing with the male, the female starts massive egg production, which causes pathology. Praziquantel (PZQ) is the only drug used, but it has an inherent risk of resistance development. Therefore, alternatives are needed. In the context of drug repurposing, the cancer drug imatinib was tested, showing high efficacy against S. mansoni in vitro. Besides the gonads, imatinib mainly affected the integrity of the intestine in males and females. In this study, we investigated the potential uptake and distribution of imatinib in adult schistosomes including its distribution kinetics. To this end, we applied for the first time atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) for drug imaging in paired S. mansoni. Our results indicate that imatinib was present in the esophagus and intestine of the male as early as 20 min after in vitro exposure, suggesting an oral uptake route. After one hour, the drug was also found inside the paired female. The detection of the main metabolite, N-desmethyl imatinib, indicated metabolization of the drug. Additionally, a marker signal for the female ovary was successfully applied to facilitate further conclusions regarding organ tropism of imatinib. Our results demonstrate that AP-SMALDI MSI is a useful method to study the uptake, tissue distribution, and metabolization of imatinib in S. mansoni. The results suggest using AP-SMALDI MSI also for investigating other antiparasitic compounds and their metabolites in schistosomes and other parasites. Graphical abstract

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Diane G. Edmondson ◽  
Steven J. Norris
Keyword(s):  
Treponema Pallidum ◽  
In Vitro Cultivation
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