The in vitro cultivation of Hymenolepis microstoma from cysticercoid to egg-producing adult

1970 ◽  
Vol 48 (5) ◽  
pp. 1135-1137 ◽  
Author(s):  
W. S. Evans
Keyword(s):  
Growth And Development ◽  
Egg Production ◽  
Liver Extract ◽  
Horse Serum ◽  
Body Growth ◽  
In Vitro Cultivation ◽  
General Body ◽  
Hymenolepis Microstoma

Cysticercoids of Hymenolepis microstoma have been cultured in vitro to egg-producing adults in 16 days using Eagle's medium with horse serum plus sheep or hamster liver extract, and ox bile. Bile was essential for egg production but not for general body growth. Worms became gravid when cultured under 0, 5, and 10% oxygen. However, growth and development was greatly inhibited when higher concentrations of oxygen were used.

scholarly journals Optimization of Conditions for In Vitro Culture of the Microphallid DigeneanGynaecotyla adunca

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Jenna West ◽  
Alexandra Mitchell ◽  
Oscar J. Pung
Keyword(s):  
Egg Production ◽  
Horse Serum ◽  
Calf Serum ◽  
Culture Conditions ◽  
In Vitro Cultivation ◽  
Ilyanassa Obsoleta ◽  
Uca Pugnax ◽  
Newborn Calf Serum ◽  
Chicken Serum

In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode,Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs,Uca pugnax, excysted in trypsin, were incubated overnight to permit fertilization, and were cultured in different conditions to find those that resulted in maximum worm longevity and egg production. When cultured in media lacking serum, worms lived longer in Hanks balanced salt solution and Dulbecco’s Modified Eagle medium/F-12 (DME/F-12) than in RPMI-1640 but produced the most eggs in DME/F-12. Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum. Horse serum was chosen over chicken serum due to the formation of a precipitate in chicken serum. The optimal concentration of horse serum with respect to egg production ranged from 5 to 20%. Infectivity of eggs deposited by worms in culture was tested by feeding eggs to mud snails,Ilyanassa obsoleta. None of these snails producedG. aduncacercariae.

scholarly journals Oviposition by Schistosoma mansoni during in vitro cultivation

1996 ◽  
Vol 38 (6) ◽  
pp. 423-426 ◽  
Author(s):  
Leo Roberto Barth ◽  
Ana Paula Morais Fernandes ◽  
Vanderlei Rodrigues
Keyword(s):  
Schistosoma Mansoni ◽  
Egg Production ◽  
Culture Medium ◽  
Parasite Development ◽  
In Vitro Cultivation ◽  
Progressive Reduction ◽  
Maximum Period ◽  
Medium Time ◽  
Kinetics Of

Observation of Schistosoma mansoni oviposition during in vitro culture of adult worms for a maximum period of 10 days showed three well distinct phases in the kinetics of oviposition: an initial phase with low egg production, a period of maximum oviposition and finally a progressive reduction in the number of eggs during the late phases of culture. The kinetics of oviposition and the number of eggs laid by the parasites are influenced by the number of worm pairs per amount of RPMI 1640 medium, time of parasite development in the vertebrate host and type of serum utilized in the culture medium.

Preliminary experiments on the in vitro cultivation of an amphistome Orthocoelium scoliocoelium (Trematoda: Digenea)

1982 ◽  
Vol 56 (3) ◽  
pp. 169-176 ◽  
Author(s):  
A. N. Sharma ◽  
P. N. Sharma
Keyword(s):  
Amino Acids ◽  
Egg Production ◽  
Rumen Fluid ◽  
Inorganic Salts ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Physiological Alterations

ABSTRACTA medium containing inorganic salts, vitamins and amino-acids is described for the in vitro cultivation of the amphistome, Orthocoelium scoliocoelium, from the rumen of buffalo. Based on the properties of rumen fluid, this medium promoted and sustained normal egg production, at 37°C temperature and pH 7·4 to 7·8 for 15 days. In contrast to other media used, no patho-physiological alterations except abnormal cytoplasmic lipid, were detected. Since the parasites produced normal eggs, it is concluded that this medium is suitable for short-term cultivation of the parasites.

scholarly journals Propagación vegetativa in vitro a partir de hojas jóvenes de caña de azúcar (Saccharum officinarum L.)

2016 ◽  
Vol 8 (1) ◽  
pp. 74
Author(s):  
José M. Cuellar
Keyword(s):  
El Salvador ◽  
Acid Concentration ◽  
Growth And Development ◽  
Saccharum Officinarum ◽  
The Other ◽  
In Vitro Cultivation ◽  
Young Leaves ◽  
Macro Nutrients

This paper reports on the first in vitro cultivation tests done in El Salvador. The responses of cultivars MEX 70-485 and MEX 68-P-23 on various propagation stages, were evaluated in vitro. Young leaves still in the rolled stage were utilized. Explants 5 mm in lenght were placed on a Murashige and Skoog (1962) medium, modified for each stage of planttet recovery as follows: For the development and growth of “calluses” dosis, 2.0, 2.5 and 3.0 mg/l of 2.4/D were added and for differentiation, it was subtracted. To induce rhyzogenesis we used a medium with half of the macro-nutrients , one with the complete salts the other with the complete salts plus a supplement of 5 mg/l of AIA. Outstanding results were the following: In the phase of “calluses” formation, the major problem was the phenolization of the explants; the best 2.4-D acid concentration was 3.0 mgl, for both the calluses formation as for their growth and development. The differentiation was obtained with the renoval of 2.4-D acid from the medium, and the rhyzogenesis was favored with the supplement of 5 mg/l of AIA

scholarly journals The Effect of Artificial Media and Temperature on the Growth and Development of the Honey Bee Brood Pathogen Ascosphaera apis

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 431
Author(s):  
Petr Mráz ◽  
Marian Hýbl ◽  
Marek Kopecký ◽  
Andrea Bohatá ◽  
Jana Konopická ◽  
...  
Keyword(s):  
Honey Bee ◽  
Growth And Development ◽  
Specific Pattern ◽  
In Vitro Cultivation ◽  
Mating Types ◽  
The European Union ◽  
Ascosphaera Apis ◽  
Reproductive Structures ◽  
Different Temperatures

Ascosphaera apis is a causative agent of chalkbrood, which is one of the most widespread honey bee diseases. In our experiments, the influence of several artificial media and cultivation under different temperatures was evaluated. Concretely, the radial growth of separated mating types was measured, reproductive structures in a Neubauer hemocytometer chamber were counted simultaneously, and the morphometry of spore cysts and spore balls was assessed. The complex set of experiments determined suitable cultivation conditions. A specific pattern between reproductive structure size and temperature was found. The optimal temperature for both mating types was 30 °C. SDA and YGPSA media are suitable for fast mycelial growth. Moreover, the effect of bee brood on fungus growth and development in vitro was investigated by modification of culture medium. The newly modified medium PDA-BB4 was most effective for the production of the reproductive structures. The result suggests that honey bee brood provides necessary nutrients for proper fungus development during in vitro cultivation. As there is no registered therapeutic agent against chalkbrood in most countries, including the European Union, the assessment of A. apis growth and development in different conditions could help to understand fungus pathogenesis and thus control chalkbrood disease.

In Vitro Cultivation of the Fetal Guinea Pig Inner Ear

1981 ◽  
Vol 90 (3) ◽  
pp. 246-250 ◽  
Author(s):  
Gustave L. Davis ◽  
Mary M. Hawrisiak
Keyword(s):  
Guinea Pig ◽  
Inner Ear ◽  
Gestational Age ◽  
Growth And Development ◽  
Progressive Increase ◽  
In Vitro Cultivation ◽  
Large Size ◽  
Electron Microscopes

Fetal guinea pig inner ears, gestational age from 21 to 54 days, were cultivated in vitro and then observed with light and electron microscopes. Early 21-day gestation otocysts developed cartilaginous capsules from differentiating mesenchyme and discrete neurosensory structures from endolabyrinthine epithelium. Coiling of the cochlea occurred. Older, differentiated inner ears showed progressive increase in size and development of the labyrinth. The 26-day gestation cochlea, maintained for 12 days in vitro, doubled in size with development comparable to the 37-day gestation in vivo control. The large size of the guinea pig inner ear resulted in varying degrees of necrosis. However, the well-defined growth and development of the guinea pig fetal inner ear in vitro indicates the efficacy of the technique and suggests that similar success would be forthcoming with other animals used in audiologic research, such as chinchilla and hamster.

Cultivation as an Estimate for Infectivity of Larval Anisakis simplex from Processed Herring

1988 ◽  
Vol 51 (9) ◽  
pp. 734-736 ◽  
Author(s):  
JADIGWA GRABDA ◽  
JEFFREY W. BIER
Keyword(s):  
Salt Concentration ◽  
Liver Extract ◽  
Anisakis Simplex ◽  
In Vitro Cultivation ◽  
Bovine Blood ◽  
Baltic Herring ◽  
Fish Flesh ◽  
Edible Fish ◽  
Spontaneous Motion

In vitro cultivation was used to estimate viability and potential infectivity of anisakine nematodes in edible fish. Salted and spiced Baltic herring were examined by dissection for the presence of larval Anisakis simplex. Stage-3 larvae, dissected from herring, were incubated for 4 d at 37°C in a medium containing liver extract and fresh bovine blood to enable dormant larvae to display spontaneous motion. In some instances movement became apparent only on days 2 or 3 in vitro. Larvae able to develop in vitro through one molt in these cultures were considered to have greater potential for causing disease in consumers than those that moved but did not molt. Mortality of larvae in fish increased as fish brining increased from week 1 to week 4, when it reached 100%. Salt concentration in the fish flesh also increased with time: 1 week, 5.6–8.2%; 2 weeks, 9.6–12.9%, 3 weeks, 11.6–14.0%; 4 weeks, 12.2–14.6%.

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