Sheep Liver Fluke, Fasciola hepatica L., 1758 from Buffalo, Bison bison (L., 1758) in Western Wyoming

1967 ◽  
Vol 53 (4) ◽  
pp. 724 ◽  
Author(s):  
Robert C. Bergstrom
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Bison Bison ◽  
Sheep Liver ◽  
Sheep Liver Fluke

The excretory metabolism of the endoparasitic digenean Fasciola hepatica and its relationship to its respiratory metabolism

Parasitology ◽  
1970 ◽  
Vol 60 (1) ◽  
pp. 1-19 ◽  
Author(s):  
G. D. Moss
Keyword(s):  
Lactic Acid ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Hymenolepis Diminuta ◽  
Respiratory Metabolism ◽  
Sheep Liver Fluke ◽  
Liver Flukes ◽  
Meat Market ◽  
Veterinary Officer

SUMMARYThe amounts of ammonia excreted by two endoparasitic flatworms have been compared and, weight for weight, the sheep liver fluke Fasciola hepatica produces ten times as much ammonia as the rat tapeworm Hymenolepis diminuta. This is thought to reflect a difference in diet, the fluke feeding mainly on the blood and tissue proteins of its host, and the tapeworm mainly on carbohydrates from the food materials in the gut of the host. However, the addition of glucose to the saline in which the flukes are cultured produces a marked decrease in the ammonia and an increase in the lactic acid excreted, suggesting that flukes are not obligatory protein feeders but that they may utilize carbohydrate when it is available. The relatively low production of lactic acid when proteins are being metabolized suggests that the flukes are producing an alternative end-product of respiratory metabolism. It is possible that this is the neutral lipid found in the excretory system of Fasciola and the possibility of an alternative metabolic pathway for the production of such lipid is discussed. The respiratory metabolism appears to be independent of oxygen, and the possible role of oxygen in other aspects of the metabolism of the fluke is discussed.I should like to express my sincere gratitude to Dr J. Llewellyn for his constant advice and encouragement during the course of this work and the preparation of the manuscript. I also thank Professor 0. E. Lowenstein, F.R.S., for allowing me the use of the facilities in his department during the tenure of an S.R.C. Research Studentship. Finally, my thanks are due to Mr E. Wilson, the Veterinary Officer at the Birmingham City Meat Market, for his constant co-operation in obtaining supplies of liver flukes.

scholarly journals The soluble glutathione transferase superfamily: role of Mu class in triclabendazole sulphoxide challenge in Fasciola hepatica

2021 ◽  
Vol 120 (3) ◽  
pp. 979-991
Author(s):  
Rebekah B. Stuart ◽  
Suzanne Zwaanswijk ◽  
Neil D. MacKintosh ◽  
Boontarikaan Witikornkul ◽  
Peter M. Brophy ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Liver Fluke ◽  
Glutathione Transferase ◽  
Fasciola Hepatica ◽  
Affinity Purification ◽  
Economic Loss ◽  
Differential Abundance ◽  
Parasitic Helminths

AbstractFasciola hepatica (liver fluke), a significant threat to food security, causes global economic loss for the livestock industry and is re-emerging as a foodborne disease of humans. In the absence of vaccines, treatment control is by anthelmintics; with only triclabendazole (TCBZ) currently effective against all stages of F. hepatica in livestock and humans. There is widespread resistance to TCBZ and its detoxification by flukes might contribute to the mechanism. However, there is limited phase I capacity in adult parasitic helminths with the phase II detoxification system dominated by the soluble glutathione transferase (GST) superfamily. Previous proteomic studies have demonstrated that the levels of Mu class GST from pooled F. hepatica parasites respond under TCBZ-sulphoxide (TCBZ-SO) challenge during in vitro culture ex-host. We have extended this finding by exploiting a sub-proteomic lead strategy to measure the change in the total soluble GST profile (GST-ome) of individual TCBZ-susceptible F. hepatica on TCBZ-SO-exposure in vitro culture. TCBZ-SO exposure demonstrated differential abundance of FhGST-Mu29 and FhGST-Mu26 following affinity purification using both GSH and S-hexyl GSH affinity. Furthermore, a low or weak affinity matrix interacting Mu class GST (FhGST-Mu5) has been identified and recombinantly expressed and represents a new low-affinity Mu class GST. Low-affinity GST isoforms within the GST-ome was not restricted to FhGST-Mu5 with a second likely low-affinity sigma class GST (FhGST-S2) uncovered. This study represents the most complete Fasciola GST-ome generated to date and has supported the potential of subproteomic analyses on individual adult flukes.

Prevalence and pathology of liver fluke (Fasciola hepatica) in fallow deer (Dama dama)

2021 ◽  
Vol 293 ◽  
pp. 109427
Author(s):  
Jane Lamb ◽  
Emma Doyle ◽  
Jamie Barwick ◽  
Michael Chambers ◽  
Lewis Kahn
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fallow Deer ◽  
Dama Dama

scholarly journals Diagnosis of Liver Fluke (Fasciola hepatica) Infection in Human Beings by Means of Immunoelectrophoresis

Nature ◽  
1963 ◽  
Vol 198 (4876) ◽  
pp. 204-204 ◽  
Author(s):  
D. TEODOROVIĆ ◽  
I. BERKEŠ ◽  
M. MILOVANAVIĆ
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Human Beings ◽  
Hepatica Infection

Spermatogenesis and the fine structure of the mature spermatozoon of the liver fluke, Fasciola hepatica (Trematoda: Digenea)

Parasitology ◽  
1990 ◽  
Vol 101 (3) ◽  
pp. 395-407 ◽  
Author(s):  
A. W. Stitt ◽  
I. Fairweather
Keyword(s):  
Fine Structure ◽  
Liver Fluke ◽  
Central Body ◽  
Fasciola Hepatica ◽  
Mature Spermatozoon ◽  
Basal Bodies ◽  
Nuclear Region ◽  
Sperm Ultrastructure ◽  
Transmission Electron ◽  
Median Process

SUMMARYSpermatogenesis and the fine structure of the mature spermatozoon of Fasciola hepatica have been studied by transmission electron microscopy. The primary spermatogonia display a typical gonial morphology and occupy the periphery of the testis. They undergo 3 mitotic divisions to give rise to 8 primary spermatocytes forming a rosette of cells connected to a central cytophore. The primary spermatocytes undergo 2 meiotic divisions, resulting in 32 spermatids that develop into spermatozoa. Intranuclear synaptonemal complexes in primary spermatocytes confirm the first meiotic division. The onset of spermiogenesis is marked by the formation of the zone of differentiation which contains 2 basal bodies and a further centriole derivative, the central body. The zone extends away from the spermatid cell to form the median process; into this migrates the differentiated and elongate nucleus. Simultaneously, 2 axonemes develop from the basal bodies. During development, they rotate through 90° to extend parallel to the median process. The migration of the nucleus to the distal end of the median process coincides with the fusion of the axonemes to the latter to form a monopartite spermatozoon. The mature spermatozoon possesses 2 axonemes of the 9 + ‘1’ pattern typical of parasitic platyhelminths, 2 elongate mitochondria and a variable array of peripheral microtubules. The nuclear region of the spermatozoon is immotile. The value of sperm ultrastructure as a taxonomic tool in platyhelminth phylogeny is discussed.

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