scholarly journals Diagnosis of Liver Fluke (Fasciola hepatica) Infection in Human Beings by Means of Immunoelectrophoresis

Nature ◽  
1963 ◽  
Vol 198 (4876) ◽  
pp. 204-204 ◽  
Author(s):  
D. TEODOROVIĆ ◽  
I. BERKEŠ ◽  
M. MILOVANAVIĆ
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Human Beings ◽  
Hepatica Infection

Innate and adaptive resistance of Indonesian Thin Tail sheep to liver fluke: A comparative analysis of Fasciola gigantica and Fasciola hepatica infection

2011 ◽  
Vol 178 (3-4) ◽  
pp. 264-272 ◽  
Author(s):  
Jill Pleasance ◽  
Herman W. Raadsma ◽  
S.E. Estuningsih ◽  
S. Widjajanti ◽  
Els Meeusen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fasciola Gigantica ◽  
Hepatica Infection ◽  
Adaptive Resistance

PRODUCTION EFFECTS OF LIVER FLUKE (FASCIOLA HEPATICA) INFECTION IN BEEF CATTLE

1980 ◽  
Vol 56 (12) ◽  
pp. 588-592 ◽  
Author(s):  
B. F. Chick ◽  
O. R. Coverdale ◽  
A. R. B. Jackson
Keyword(s):  
Beef Cattle ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Hepatica Infection ◽  
Production Effects

scholarly journals The soluble glutathione transferase superfamily: role of Mu class in triclabendazole sulphoxide challenge in Fasciola hepatica

2021 ◽  
Vol 120 (3) ◽  
pp. 979-991
Author(s):  
Rebekah B. Stuart ◽  
Suzanne Zwaanswijk ◽  
Neil D. MacKintosh ◽  
Boontarikaan Witikornkul ◽  
Peter M. Brophy ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Liver Fluke ◽  
Glutathione Transferase ◽  
Fasciola Hepatica ◽  
Affinity Purification ◽  
Economic Loss ◽  
Differential Abundance ◽  
Parasitic Helminths

AbstractFasciola hepatica (liver fluke), a significant threat to food security, causes global economic loss for the livestock industry and is re-emerging as a foodborne disease of humans. In the absence of vaccines, treatment control is by anthelmintics; with only triclabendazole (TCBZ) currently effective against all stages of F. hepatica in livestock and humans. There is widespread resistance to TCBZ and its detoxification by flukes might contribute to the mechanism. However, there is limited phase I capacity in adult parasitic helminths with the phase II detoxification system dominated by the soluble glutathione transferase (GST) superfamily. Previous proteomic studies have demonstrated that the levels of Mu class GST from pooled F. hepatica parasites respond under TCBZ-sulphoxide (TCBZ-SO) challenge during in vitro culture ex-host. We have extended this finding by exploiting a sub-proteomic lead strategy to measure the change in the total soluble GST profile (GST-ome) of individual TCBZ-susceptible F. hepatica on TCBZ-SO-exposure in vitro culture. TCBZ-SO exposure demonstrated differential abundance of FhGST-Mu29 and FhGST-Mu26 following affinity purification using both GSH and S-hexyl GSH affinity. Furthermore, a low or weak affinity matrix interacting Mu class GST (FhGST-Mu5) has been identified and recombinantly expressed and represents a new low-affinity Mu class GST. Low-affinity GST isoforms within the GST-ome was not restricted to FhGST-Mu5 with a second likely low-affinity sigma class GST (FhGST-S2) uncovered. This study represents the most complete Fasciola GST-ome generated to date and has supported the potential of subproteomic analyses on individual adult flukes.

Prevalence and pathology of liver fluke (Fasciola hepatica) in fallow deer (Dama dama)

2021 ◽  
Vol 293 ◽  
pp. 109427
Author(s):  
Jane Lamb ◽  
Emma Doyle ◽  
Jamie Barwick ◽  
Michael Chambers ◽  
Lewis Kahn
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fallow Deer ◽  
Dama Dama

scholarly journals Comparison of two coprological methods for the veterinary diagnosis of fasciolosis

2005 ◽  
Vol 57 (2) ◽  
pp. 181-185 ◽  
Author(s):  
F. Kleiman ◽  
S. Pietrokovsky ◽  
S. Gil ◽  
C. Wisnivesky-Colli
Keyword(s):  
Endemic Area ◽  
Diagnostic Method ◽  
Staining Method ◽  
Fasciola Hepatica ◽  
False Negative ◽  
Sample Processing ◽  
Hepatica Infection ◽  
Sedimentation Method ◽  
Faecal Samples ◽  
Reliable Diagnostic Method

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.

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