Effects of Low Molecular Weight Serum Glycoproteins on Granulopoiesis in Rat Bone Marrow

1974 ◽  
Vol 93 (2) ◽  
pp. 226
Author(s):  
Robert L. K. Tjan ◽  
James D. Graham
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
Low Molecular Weight ◽  
Rat Bone

P2Z purinoceptor-associated pores induced by extracellular ATP in macrophages and J774 cells

1997 ◽  
Vol 273 (6) ◽  
pp. C1793-C1800 ◽  
Author(s):  
Robson Coutinho-Silva ◽  
Pedro Muanis Persechini
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
Patch Clamp ◽  
Plasma Membranes ◽  
Extracellular Atp ◽  
Low Molecular Weight ◽  
Whole Cell ◽  
Voltage Dependent ◽  
J774 Cells ◽  
Bone Marrow Derived Cells

Millimolar concentrations of extracellular ATP (ATPo) can induce the permeabilization of plasma membranes of macrophages and other bone marrow-derived cells to low-molecular-weight solutes, a phenomenon that is the hallmark of P2Z purinoceptors. However, patch-clamp and whole cell electrophysiological experiments have so far failed to demonstrate the existence of any ATPo-induced P2Z-associated pores underlying this permeabilization phenomenon. Here, we describe ATPo-induced pores of 409 ± 33 pS recorded using cell-attached patch-clamp experiments performed in macrophages and J774 cells. These pores are voltage dependent and display several properties of the P2Z-associated permeabilization phenomenon: they are permeable to both large cations and anions, such as tris(hydroxymethyl)aminomethane, N-methyl-d-glucamine, and glutamate; their opening is favored at temperatures higher than 30°C; they are blocked by oxidized ATP and Mg2+; and they can be triggered by 3′- O-(4-benzoylbenzoyl)-ATP but not by UTP or ADP. We conclude that the pores described in this report are associated with the P2Z permeabilization phenomenon.

scholarly journals The separation of Antler Polypeptide and its effects on the proliferation and osteogenetic differentiation of Bone Marrow Mesenchymal Stem Cells

2020 ◽  
Author(s):  
Wang Ping ◽  
Sun Tie-Feng ◽  
Li Gang ◽  
Zhang Hui-Min ◽  
Liu Fan-jie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Weight ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
High Performance ◽  
The Other ◽  
Proliferation Index ◽  
Blank Group ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

AbstractThe effects of antler polypeptide on rat bone marrow mesenchymal stem cells (BMSCs) were investigated. Antler polypeptide was separated from Colla Cornus Cervi by ultrafiltration into different samples according to molecular weight: A (molecular weight <800 Da), B (molecular weight 800-1500 Da) and C (molecular weight >1500 Da). The content of antler polypeptide in A, B and C solutions were quantified by high-performance liquid chromatography (HPLC). The effects of antler polypeptide at different concentrations on the proliferation, cell cycle, and osteogenesis of BMSCs were investigated. The highest cell proliferation rate (84.66%) was observed for antler polypeptide B at a concentration of 1.578 × 10−2 g/mL. Antler polypeptide B significantly promoted the proliferation of BMSCs with a proliferation index of 38.68%, which was significantly higher than that of the other groups. Antler polypeptide B significantly enhanced the activity of alkaline phosphatase in BMSCs compared to that of blank group (P <0.001). Antler polypeptide B increased the BMP7 protein expression in BMSCs. Our data suggested that antler polypeptide may promote the proliferation and osteogenic differentiation of BMSCs.

PBI-1402: A Low Molecular Weight Synthetic Hematopoietic Growth Stimulant.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4222-4222 ◽  
Author(s):  
Brigitte Grouix ◽  
Nathalie Julien ◽  
Mouna Lagraoui ◽  
Marie-Josée Morin ◽  
Gorazd Krosl ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
Marrow Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Low Molecular Weight ◽  
Hematopoietic Progenitors ◽  
Gm Csf ◽  
Growth Stimulant

Abstract PBI-1402 is a non-toxic, well-defined low molecular weight synthetic hematopoietic growth stimulant. PBI-1402 promotes the proliferation and maturation of hematopoietic progenitors (myeloid and erythroid populations) yielding a biological efficacy comparable to G-CSF, GM-CSF and EPO in in vitro human bone marrow cell proliferation and colony formation assays. An additive effect is observed when PBI-1402 is combined with G-CSF, GM-CSF and EPO. In human bone marrow colony assay, PBI-1402 enhances the differentiation of pluripotent stem cells: CFU-GEMM, CFU-GM with a predominant effect on BFU-E. Furthermore, PBI-1402 exerts its activity via a different mechanism of action than EPO and stem cell factor (SCF) and at an earlier stage on more immature hematopoietic progenitors. PBI-1402 is targeted as an adjunct to cancer chemo/radiotherapy, bone marrow transplantation and diseases involving neutropenia and anemia.

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