Effect of high- and low-molecular-weight soluble bone marrow factors on antibody formation and pain sensitivity in animals

1990 ◽  
Vol 109 (1) ◽  
pp. 72-75 ◽  
Author(s):  
O. G. Yanoskii ◽  
L. A. Zakharova ◽  
A. M. Vasilenko
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
Antibody Formation ◽  
Pain Sensitivity ◽  
Low Molecular Weight

P2Z purinoceptor-associated pores induced by extracellular ATP in macrophages and J774 cells

1997 ◽  
Vol 273 (6) ◽  
pp. C1793-C1800 ◽  
Author(s):  
Robson Coutinho-Silva ◽  
Pedro Muanis Persechini
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
Patch Clamp ◽  
Plasma Membranes ◽  
Extracellular Atp ◽  
Low Molecular Weight ◽  
Whole Cell ◽  
Voltage Dependent ◽  
J774 Cells ◽  
Bone Marrow Derived Cells

Millimolar concentrations of extracellular ATP (ATPo) can induce the permeabilization of plasma membranes of macrophages and other bone marrow-derived cells to low-molecular-weight solutes, a phenomenon that is the hallmark of P2Z purinoceptors. However, patch-clamp and whole cell electrophysiological experiments have so far failed to demonstrate the existence of any ATPo-induced P2Z-associated pores underlying this permeabilization phenomenon. Here, we describe ATPo-induced pores of 409 ± 33 pS recorded using cell-attached patch-clamp experiments performed in macrophages and J774 cells. These pores are voltage dependent and display several properties of the P2Z-associated permeabilization phenomenon: they are permeable to both large cations and anions, such as tris(hydroxymethyl)aminomethane, N-methyl-d-glucamine, and glutamate; their opening is favored at temperatures higher than 30°C; they are blocked by oxidized ATP and Mg2+; and they can be triggered by 3′- O-(4-benzoylbenzoyl)-ATP but not by UTP or ADP. We conclude that the pores described in this report are associated with the P2Z permeabilization phenomenon.

PBI-1402: A Low Molecular Weight Synthetic Hematopoietic Growth Stimulant.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4222-4222 ◽  
Author(s):  
Brigitte Grouix ◽  
Nathalie Julien ◽  
Mouna Lagraoui ◽  
Marie-Josée Morin ◽  
Gorazd Krosl ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
Marrow Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Low Molecular Weight ◽  
Hematopoietic Progenitors ◽  
Gm Csf ◽  
Growth Stimulant

Abstract PBI-1402 is a non-toxic, well-defined low molecular weight synthetic hematopoietic growth stimulant. PBI-1402 promotes the proliferation and maturation of hematopoietic progenitors (myeloid and erythroid populations) yielding a biological efficacy comparable to G-CSF, GM-CSF and EPO in in vitro human bone marrow cell proliferation and colony formation assays. An additive effect is observed when PBI-1402 is combined with G-CSF, GM-CSF and EPO. In human bone marrow colony assay, PBI-1402 enhances the differentiation of pluripotent stem cells: CFU-GEMM, CFU-GM with a predominant effect on BFU-E. Furthermore, PBI-1402 exerts its activity via a different mechanism of action than EPO and stem cell factor (SCF) and at an earlier stage on more immature hematopoietic progenitors. PBI-1402 is targeted as an adjunct to cancer chemo/radiotherapy, bone marrow transplantation and diseases involving neutropenia and anemia.

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