Analysis of Epidemic and Endemic Isolates of Xanthomonas maltophilia by Contour-Clamped Homogeneous Electric Field Gel Electrophoresis

1994 ◽  
Vol 15 (11) ◽  
pp. 691-696 ◽  
Author(s):  
Carolyn VanCouwenberghe ◽  
Stuart Cohen
Keyword(s):  
Electric Field ◽  
Gel Electrophoresis ◽  
Homogeneous Electric Field ◽  
Xanthomonas Maltophilia

Analysis of Epidemic and Endemic Isolates of Xanthomonas maltophilia by Contour-Clamped Homogeneous Electric Field Gel Electrophoresis

1994 ◽  
Vol 15 (11) ◽  
pp. 691-696 ◽  
Author(s):  
Carolyn VanCouwenberghe ◽  
Stuart Cohen
Keyword(s):  
Intensive Care ◽  
Electric Field ◽  
Gel Electrophoresis ◽  
Spatial Proximity ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Xanthomonas Maltophilia ◽  
Intensive Care Nursery ◽  
Mode Of Transmission ◽  
Temporal And Spatial

AbstractBackground:Xanthomonas maltophiliais increasingly a cause of nosocomial infections. The mode of transmission of this organism is not well known.Objective:To investigate clonality ofX maltophiliaisolates in epidemic and endemic settings.Methods:An outbreak ofX maltophiliawas noted in the Intensive Care Nursery (ICN). Over the ensuing 9 months, hospitalwide isolates ofX maltophiliawere analyzed using contour-clamped homogeneous electric field (CHEF) gel electrophoresis of chromosomal DNA. This method was compared with the antibiogram for detecting differences and similarities among strains.Results:X maltophiliawas recovered from 76 sites in 72 patients; 65 isolates from 61 patients and the hands of one nurse were available for analysis. CHEF demonstrated differences between most epidemiologically unrelated strains and similarity between most epidemiologically related strains. Several strains, initially presumed to be related because of temporal and spatial proximity of the patients involved, were determined by CHEF analysis to be independent infections. One pair of isolates whoseXbalCHEF patterns differed by a single band were differentiated clearly by Sspl. There was enough variation in the minimum inhibitory concentrations of selected antibiotics to allow typing of some strains. The antibiogram, however, did not group all of the ICN outbreak isolates with others found to be genetically identical by CHEF and it grouped 39 of 56 isolates with others not genetically the same.Conclusions:Although it is a convenient and economical tool, the antibiogram has limitations. Analysis by CHEF should help to elucidate the epidemiological spread ofX maltophiliain the hospital.

Rapid Protocol for Characterizing Klebsiella pneumoniae Isolates by Pulsed Field Gel Electrophoresis (PFGE) in Contour Clamped Homogeneous Electric Field (CHEF) Minigels

2020 ◽  
pp. 1-11
Author(s):  
Maribel Santiago Rodríguez ◽  
Juan C. Bravata Alcántara ◽  
Juan C. Martínez Briseño ◽  
Eduardo Díaz Escamilla ◽  
Ileana A. Cortes Ortiz ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Electric Field ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Homogeneous Electric Field ◽  
Pulsed Field

Evaluation of Propionibacterium acnes Isolates Using Contour-clamped Homogeneous Electric Field Gel Electrophoresis

Anaerobe ◽  
1999 ◽  
Vol 5 (6) ◽  
pp. 579-582 ◽  
Author(s):  
T Daniel Ting ◽  
Kenneth C Chern ◽  
David M Meisler ◽  
Gerri S Hall ◽  
Cindy C Knapp ◽  
...  
Keyword(s):  
Electric Field ◽  
Gel Electrophoresis ◽  
Propionibacterium Acnes ◽  
Homogeneous Electric Field
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