Brief Report: Bactericidal Effects of Antiseptics and Disinfectants against Methicillin-Resistant Staphylococcus aureus

1989 ◽  
Vol 10 (12) ◽  
pp. 562-564 ◽  
Author(s):  
Hiroyoshi Kobayashi ◽  
Masakazu Tsuzuki ◽  
Kazunari Hosobuchi
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Bactericidal Effects

scholarly journals Bacteriostatic and Bactericidal effects of ethyl acetate root bark extract of Terminalia avicennioides on Methicillin-resistant Staphylococcus aureus

2018 ◽  
Vol 12 (5) ◽  
pp. 45-54
Author(s):  
Ekene Ugochukwu Adim ◽  
Emeka John Dingwoke ◽  
Fatima Amin Adamude ◽  
Chidi Edenta ◽  
Ndubuisi Nwobodo Nwobodo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bark Extract ◽  
Root Bark ◽  
Methicillin Resistant ◽  
Bactericidal Effects

scholarly journals Bactericidal Effects of Low-Irradiance Low Level Light Therapy on Methicillin-Resistant Staphylococcus Aureus in Vitro

Fine Focus ◽  
2016 ◽  
Vol 2 (2) ◽  
pp. 104-115
Author(s):  
Patrick J. McMullan ◽  
Jakob Krzyston ◽  
Robert Osgood
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Load ◽  
Light Therapy ◽  
Methicillin Resistant ◽  
Low Level Light Therapy ◽  
Bactericidal Properties ◽  
Bactericidal Effects ◽  
Total Fluence

Low Level Light Therapy (LLLT) within the visible blue spectrum (400-470 nanometers) is a well-documented therapeutic alternative to combat multidrug resistant organism infections through the generation of reactive oxygen species (ROS). However, one shortcoming of LLLT is that many studies deliver therapy through high powered lasers and lamps. High powered light sources not only require specialized staff to operate, but they also deliver the total light dose (fluence) at an exceptionally high intensity, or irradiance, which could consequently deplete the oxygen supplies required to promote LLLT’s bactericidal properties. To overcome these faults, low-irradiance LLLT, or delivering the same total fluence of LLLT over an extended period of time with decreased irradiance was evaluated in vitro. To further explore this alternative approach, the bactericidal effects of low-irradiance (10.44 mW/cm2) LLLT using wavelengths of 405-nm, 422-nm and 470-nm were studied on methicillin-resistant Staphylococcus aureus (MRSA) cultures. Among these wavelengths, it was determined that 405-nm LLLT provided the most effective reduction of bacterial load at the lowest total fluence (75 J/cm2) (94.50% reduction). The bactericidal effects of 405-nm low-irradiance LLLT were then further studied by treating MRSA cultures to 75 J/cm2 LLLT while using irradiances of 5.22 mW/cm2 and 3.48 mW/cm2. It was concluded that there was a greater reduction of MRSA bacterial load when samples were exposed to irradiances of 5.22 mW/cm2 (95.71% reduction) and 3.48 mW/cm2 (99.63% reduction). This study validates the bactericidal properties of low-irradiance LLLT on MRSA, and subsequent studies should be completed to optimize its full therapeutic potential.

scholarly journals Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA)

2019 ◽  
Vol 55 (1) ◽  
pp. 1
Author(s):  
Amaliyah Nurul Hidayah ◽  
Eddy Bagus Wasito ◽  
Kartuti Debora ◽  
Achmad Basori ◽  
Isnaeni Isnaeni ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pearson Correlation ◽  
Statistical Significance ◽  
Bactericidal Effect ◽  
Methicillin Resistant ◽  
Citronella Oil ◽  
Bactericidal Effects ◽  
Effect Test

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r=-0.956 (p=0.000), but the correlation was positive for biofilm eradication with r=0.918 (p=0.000) and anticolony spreading with r=1.000 (p=0.000).

scholarly journals Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA)

2021 ◽  
Vol 55 (1) ◽  
pp. 1
Author(s):  
Amaliyah Nurul Hidayah ◽  
Eddy Bagus Wasito ◽  
Kartuti Debora ◽  
Achmad Basori ◽  
Isnaeni Isnaeni ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pearson Correlation ◽  
Statistical Significance ◽  
Bactericidal Effect ◽  
Methicillin Resistant ◽  
Citronella Oil ◽  
Bactericidal Effects ◽  
Effect Test

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r = -0.956 (p = 0.000), but the correlation was positive for biofilm eradication with r = 0.918 (p = 0.000) and anticolony spreading with r = 1.000 (p = 0.000).

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