Farm and College; The College of Agriculture of the University of Wisconsin, A History

1953 ◽  
Vol 40 (2) ◽  
pp. 369
Author(s):  
Walton Bean ◽  
W. H. Glover
Author(s):  
Hans Ris

The High Voltage Electron Microscope Laboratory at the University of Wisconsin has been in operation a little over one year. I would like to give a progress report about our experience with this new technique. The achievement of good resolution with thick specimens has been mainly exploited so far. A cold stage which will allow us to look at frozen specimens and a hydration stage are now being installed in our microscope. This will soon make it possible to study undehydrated specimens, a particularly exciting application of the high voltage microscope.Some of the problems studied at the Madison facility are: Structure of kinetoplast and flagella in trypanosomes (J. Paulin, U. of Georgia); growth cones of nerve fibers (R. Hannah, U. of Georgia Medical School); spiny dendrites in cerebellum of mouse (Scott and Guillery, Anatomy, U. of Wis.); spindle of baker's yeast (Joan Peterson, Madison) spindle of Haemanthus (A. Bajer, U. of Oregon, Eugene) chromosome structure (Hans Ris, U. of Wisconsin, Madison). Dr. Paulin and Dr. Hanna are reporting their work separately at this meeting and I shall therefore not discuss it here.


Author(s):  
Patricia N. Hackney

Ustilago hordei and Ustilago violacea are yeast-like basidiomycete pathogens ofHordeum vulgare and Silene alba respectively. The mating type system in both species of Ustilago is bipolar, with alleles, A,a, (U.hordei) and a1, a2 (U.violacea) at a single locus. Haploid sporidia maintain the asexual phase by budding, while the sexual phase is initiated by conjugation tube formation between the mating types during budding and conjugation.For observation of budding, sporidia were prepared by culturing the four types on YEG (yeast extract glucose) broth for 24 hours. After centrifugation at 5000g cells were either left unmated or mated in a1/a2,A/a combinations. The sporidia were then mixed 1:1 with 4% agar and the resulting 1mm cubes fixed in 8% gluteraldehyde and post fixed in osmium tetroxide. After dehydration and embedding cubes were thin sectioned with a LKB ultratome and photographed in a Zeiss 9s transmission electron microscope or in an AE1 electron microscope of MK11 1MEV at the High Voltage Electron Microscopy Center of the University of Wisconsin-Madison.


Author(s):  
Neveen Anwer Abdalla

The experiment has been conducted in the nursery of the Department of Horticulture and Landscape Design, College of Agriculture, the University of Basrah to study the effect of Spraying foliar of the biostimulants Izomen and Humus on the growth and flowering of Freesia plants. The corms in similar size were planted in pots with a diameter and height of 25 cm, which filled with 2.5 kg of sterilized loam soil. After 50 days of planting, the plants sprayed with Humus at 0, 1.5, 2.5 ml L-1 and after five days sprayed with Izomen at 0, 1.5, 2.5 ml L-1 The different concentrations of biostimulants are sprayed three times, the period between one spray and another 15 is days. The results showed that the spraying of Humus at 2.5 ml L-1 significantly increased the plant height, the number of leaves and the leaf content of chlorophyll recorded (29.56 cm, 8.33 and 58.43%) respectively. Moreover, it is recorded early the flowering date (130 days), and the highest flowering mean is (2.12 inflorescence/plant) and the highest period of the remained flowers on the plant and the vase life (10 and 8 days) respectively. The effects of both Humus and Izomen were similar. In addition to the highest mean of their interaction at 2.5 ml L -1for all the studied traits.


1923 ◽  
Vol 9 (1) ◽  
pp. 81-90
Author(s):  
W. L. Westermann ◽  
A. G. Laird

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