Differentiation of Oncogenic and Nononcogenic Strains of Marek's Disease Virus Type 1 by Using Polymerase Chain Reaction DNA Amplification

1992 ◽  
Vol 36 (3) ◽  
pp. 637 ◽  
Author(s):  
Geng-Sheng Zhu ◽  
Tomoko Ojima ◽  
Takashi Hironaka ◽  
Takeshi Ihara ◽  
Noriko Mizukoshi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Virus Type ◽  
Dna Amplification ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Chain Reaction ◽  
Disease Virus Type ◽  
Polymerase Chain

scholarly journals Comparative suitability of the feathers, peripheral blood and spleen tissue samples of chickens for the detection of Marek’s disease virus by polymerase chain reaction

2019 ◽  
Vol 35 (1-2) ◽  
pp. 1-6
Author(s):  
MW Rahman ◽  
M Nooruzzaman ◽  
US Suma ◽  
EH Chowdhury ◽  
MR Islam
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Peripheral Blood ◽  
Buffy Coat ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Chain Reaction ◽  
Polymerase Chain

A total of 24 clinical specimens (10 feathers, 8 peripheral bloods and 6 spleens) were collected from 10 suspected outbreaks of Marek’s disease (MD) from Mymensingh, Tangail, Gazipur and Pabna districts of Bangladesh. A polymerase chain reaction (PCR) protocol originally described by Silva (1992) was adopted to detect Marek’s disease virus (MDV) genome in these specimens. All the tested peripheral blood buffy coat samples (100%) were positive for MDV in PCR, while 70% of feather samples and 66.6% of spleen samples were positive. A band of 317 bp size was found in all positive samples. A few samples also yielded additional bands of 185 bp size and/or multiple bands of larger than 317 bp size, indicating the presence of both virulent MDV and the vaccine virus. The study suggests that peripheral blood and feathers from live birds, and feathers from dead birds are the samples of choice for the detection of MDV by PCR. The Bangladesh Veterinarian (2018) 35(1&2): 1-6

scholarly journals Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

2000 ◽  
Vol 74 (7) ◽  
pp. 3217-3226 ◽  
Author(s):  
Kengo Sonoda ◽  
Masashi Sakaguchi ◽  
Hiroshi Okamura ◽  
Kenji Yokogawa ◽  
Eiji Tokunaga ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Maternal Antibodies ◽  
F Protein ◽  
Polyvalent Vaccine ◽  
Disease Virus Type ◽  
Commercial Chickens

ABSTRACT An earlier report (M. Sakaguchi et al., Vaccine 16:472–479, 1998) showed that recombinant Marek's disease virus type 1 (rMDV1) expressing the fusion (F) protein of Newcastle disease virus (NDV-F) under the control of the simian virus 40 late promoter [rMDV1-US10L(F)] protected specific pathogen-free chickens from NDV challenge, but not commercial chickens with maternal antibodies against NDV and MDV1. In the present study, we constructed an improved polyvalent vaccine based on MDV1 against MDV and NDV in commercial chickens with maternal antibodies. The study can be summarized as follows. (i) We constructed rMDV1 expressing NDV-F under the control of the MDV1 glycoprotein B (gB) promoter [rMDV1-US10P(F)]. (ii) Much less NDV-F protein was expressed in cells infected with rMDV1-US10P(F) than in those infected with rMDV1-US10L(F). (iii) The antibody response against NDV-F and MDV1 antigens of commercial chickens vaccinated with rMDV1-US10P(F) was much stronger and faster than with rMDV1-US10L(F), and a high level of antibody against NDV-F persisted for over 80 weeks postvaccination. (iv) rMDV1-US10P(F) was readily reisolated from the vaccinated chickens, and the recovered viruses were found to express NDV-F. (v) Vaccination of commercial chickens having maternal antibodies to rMDV1-US10P(F) completely protected them from NDV challenge. (vi) rMDV1-US10P(F) offered the same degree of protection against very virulent MDV1 as the parental MDV1 and commercial vaccines. These results indicate that rMDV1-US10P(F) is an effective and stable polyvalent vaccine against both Marek's and Newcastle diseases even in the presence of maternal antibodies.

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