In ovo Vaccination of Chicken Embryos with Experimental Newcastle Disease and Avian Influenza Oil-Emulsion Vaccines

1997 ◽  
Vol 41 (4) ◽  
pp. 856 ◽  
Author(s):  
Henry Stone ◽  
Bailey Mitchell ◽  
Max Brugh
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
In Ovo ◽  
Oil Emulsion ◽  
Chicken Embryos

scholarly journals Pathological and Immunological Study of an in ovo Complex Vaccine against Infectious Bursal Disease

2000 ◽  
Vol 48 (4) ◽  
pp. 443-454 ◽  
Author(s):  
Mária Kelemen ◽  
Katalin Forgách ◽  
Judit Iván ◽  
V. Palya ◽  
T. Süveges ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Broiler Chicken ◽  
Haemagglutination Inhibition ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Hatching Rate ◽  
Immunological Study ◽  
In Ovo ◽  
Chicken Embryos ◽  
Bursal Disease

The appearance of very virulent strains of infectious bursal disease (IBD) virus at the end of the 1980s made it necessary to develop more effective immunization procedures. To facilitate this, the immunogenicity and the immunosuppressive effect of a mild (G-87), an intermediate (LIBD) and an intermediate-plus (IBDV 2512) IBDV strain were tested after the in ovo inoculation of 18-day-old SPF and broiler chicken embryos. It was established that no noteworthy difference existed between the immunized and the control embryos in hatching rate and hatching weight. The higher the virulence of the vaccine virus strain, the more severe damage it caused to the lymphocytes of the bursa of Fabricius. In SPF chickens, the haemagglutination inhibition (HI) titres induced by a Newcastle disease (ND) vaccine administered at day old decreased in inverse ratio to the virulence of the IBD vaccine strain, while in broiler chickens this was not observed. Despite the decrease of the HI titre, the level of protection did not decline, or did so only after the use of the ‘hot’ strain. SPF chickens immunized in ovo with a complex vaccine prepared from strain IBDV 2512 and IBD antibody showed the same protection against Newcastle disease as the broilers. In broiler chicken embryos immunized in ovo, only strain IBDV 2512 induced antibody production, and such chickens were protected against IBD at 3 weeks of age. The complex vaccine administered in ovo has been used successfully at farm hatcheries as well.

scholarly journals Evaluation of In Ovo Antiviral Activities of Medicinal Flowers against Newcastle Disease Virus and Avian Influenza Virus

2021 ◽  
Vol 26 (01) ◽  
pp. 185-192
Author(s):  
Iqra Nazir
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Avian Influenza Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
In Ovo ◽  
Hemagglutination Assay ◽  
Ethanolic Extracts

In Pakistan, the poultry industry is one of the rapidly growing industries. Due to lack of biosecurity measures, this is affected by some important infectious agents such as Avian Influenza virus (H9N2) and Newcastle disease virus (NDV) results in a huge economic loss. So, to control these losses discovery of new anti-viral drugs required to bring into line to fight against these infections. It is a general perception that the active components of medicinal plants have effective results against various infections like the influenza virus. The current therapeutic facilities need to be improved by investigating new antiviral drugs from natural resources to fight against viral infections. The present study was conducted on ethanolic extracts of seven different flowers to examine their antiviral activity against NDV and H9N2 in ovo using chicken embryonated egg inoculation. The spot agglutination and hemagglutination tests showed inhibitory effects of Rosa damascena Miller, Achillea millefolium, Woodfordia fruticosa Kurtz and Bombax ceiba L. against NDV as no agglutination observed. While the extracts of Taxacum officianale Weber, Hyssopus officianalis L. and Chrysanthemum cinerafolium (Trevis.) Vis. showed positive results for both spot agglutination and hemagglutination assay against NDV. However, both spot agglutination and hemagglutination assay showed inhibitory effect of all the flowers extracts against H9N2. The bioactive components such as alkaloids, ethers, terpenoids, etc. of each flower were analyzed through Gas chromatography mass spectrometry (GC-MS). The current results revealed that ethanolic extracts of these flowers possess strong antiviral activity because of their active ingredients. These ingredients should be isolated, commercialized and used for therapeutic purpose. © 2021 Friends Science Publishers

scholarly journals A combination in-ovo vaccine for avian influenza virus and Newcastle disease virus

Vaccine ◽  
2008 ◽  
Vol 26 (4) ◽  
pp. 522-531 ◽  
Author(s):  
John Steel ◽  
Svetlana V. Burmakina ◽  
Colleen Thomas ◽  
Erica Spackman ◽  
Adolfo García-Sastre ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Avian Influenza Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
In Ovo

scholarly journals Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus

2019 ◽  
Vol 12 (1) ◽  
pp. 97-105 ◽  
Author(s):  
Ahmed Ali ◽  
Marwa Safwat ◽  
Walid H. Kilany ◽  
Abdou Nagy ◽  
Awad A. Shehata ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
Highly Pathogenic Avian Influenza ◽  
Highly Pathogenic ◽  
Virus Shedding ◽  
Oil Emulsion ◽  
Pathogenic Avian Influenza ◽  
Clinical Protection ◽  
Hpai H5n1 ◽  
Antibody Titers

Aim: The aim of the current study was to evaluate the efficacy of a trivalent-inactivated oil-emulsion vaccine against challenge by different clades highly pathogenic avian influenza (HPAI) viruses including HPAI-H5N8 and the virulent genotype VII Newcastle disease virus (NDV) (vNDV). Materials and Methods: The vaccine studied herein is composed of reassortant AI viruses rgA/Chicken/Egypt/ ME1010/2016 (clade 2.2.1.1), H5N1 rgA/Chicken/Egypt/RG-173CAL/2017 (clade 2.2.1.2), and "NDV" (LaSota NDV/ CK/Egypt/11478AF/11); all used at a concentration of 108 EID50/bird and mixed with Montanide-ISA70 oil adjuvant. Two-week-old specific pathogen free (SPF) chickens were immunized subcutaneously with 0.5 ml of the vaccine, and hemagglutination inhibition (HI) antibody titers were monitored weekly. The intranasal challenge was conducted 4 weeks post-vaccination (PV) using 106 EID50/0.1 ml of the different virulent HPAI-H5N1 viruses representing clades 2.2.1, 2.2.1.1, 2.2.1.2, 2.3.4.4b-H5N8, and the vNDV. Results: The vaccine induced HI antibody titers of >6log2 against both H5N1 and NDV viruses at 2 weeks PV. Clinical protection against all HPAI H5N1 viruses and vNDV was 100%, except for HPAI H5N1 clade-2.2.1 and HPAI H5N8 clade- 2.3.4.4b viruses that showed 93.3% protection. Challenged SPF chickens showed significant decreases in the virus shedding titers up to <3log10 compared to challenge control chickens. No virus shedding was detected 6 "days post-challenge" in all vaccinated challenged groups. Conclusion: Our results indicate that the trivalent H5ND vaccine provides significant clinical protection against different clades of the HPAI viruses including the newly emerging H5N8 HPAI virus. Availability of such potent multivalent oil-emulsion vaccine offers an effective tool against HPAI control in endemic countries and promises simpler vaccination programs.

scholarly journals CAvant® WO-60 as an Effective Immunological Adjuvant for Avian Influenza and Newcastle Disease Vaccine

2021 ◽  
Vol 8 ◽  
Author(s):  
Eun-Seo Lee ◽  
Young-Jung Shim ◽  
W. A. Gayan Chathuranga ◽  
Young-Hoon Ahn ◽  
In-Joong Yoon ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
Antibody Responses ◽  
Economic Losses ◽  
Cell Mediated Immunity ◽  
Inactivated Vaccine ◽  
Aquatic Bird ◽  
Oil Emulsion ◽  
Water In Oil Emulsion ◽  
Newcastle Disease Vaccine

Despite the immunogenicity of vaccines currently used in poultry, several pathogens, including avian influenza virus (AIV) and Newcastle disease virus (NDV), cause enormous economic losses to the global poultry industry. The efficacy of vaccines can be improved by the introduction of effective adjuvants. This study evaluated a novel water-in-oil emulsion adjuvant, CAvant® WO-60, which effectively enhanced both the immunogenicity of conserved influenza antigen sM2HA2 and inactivated whole H9N2 antigen (iH9N2). CAvant® WO-60 induced both humoral and cell-mediated immunity in mice and provided 100% protection from challenge with 10 LD50 of A/Aquatic bird/Korea/W81/2005 (H5N2) and A/Chicken/Korea/116/2004 (H9N2) AIV. Importantly, immunization of chickens with iH9N2 plus inactivated NDV LaSota (iNDV) bivalent inactivated vaccine emulsified in CAvant® WO-60 induced seroprotective levels of antigen-specific antibody responses. Taken together, these results suggested that CAvant® WO-60 is a promising adjuvant for poultry vaccines.

Sign in / Sign up

Export Citation Format

Share Document