Optimization of Hydrophile-Lipophile Balance for Improved Efficacy of Newcastle Disease and Avian Influenza Oil-Emulsion Vaccines

1988 ◽  
Vol 32 (1) ◽  
pp. 68 ◽  
Author(s):  
Henry D. Stone
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
Oil Emulsion

scholarly journals Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus

2019 ◽  
Vol 12 (1) ◽  
pp. 97-105 ◽  
Author(s):  
Ahmed Ali ◽  
Marwa Safwat ◽  
Walid H. Kilany ◽  
Abdou Nagy ◽  
Awad A. Shehata ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
Highly Pathogenic Avian Influenza ◽  
Highly Pathogenic ◽  
Virus Shedding ◽  
Oil Emulsion ◽  
Pathogenic Avian Influenza ◽  
Clinical Protection ◽  
Hpai H5n1 ◽  
Antibody Titers

Aim: The aim of the current study was to evaluate the efficacy of a trivalent-inactivated oil-emulsion vaccine against challenge by different clades highly pathogenic avian influenza (HPAI) viruses including HPAI-H5N8 and the virulent genotype VII Newcastle disease virus (NDV) (vNDV). Materials and Methods: The vaccine studied herein is composed of reassortant AI viruses rgA/Chicken/Egypt/ ME1010/2016 (clade 2.2.1.1), H5N1 rgA/Chicken/Egypt/RG-173CAL/2017 (clade 2.2.1.2), and "NDV" (LaSota NDV/ CK/Egypt/11478AF/11); all used at a concentration of 108 EID50/bird and mixed with Montanide-ISA70 oil adjuvant. Two-week-old specific pathogen free (SPF) chickens were immunized subcutaneously with 0.5 ml of the vaccine, and hemagglutination inhibition (HI) antibody titers were monitored weekly. The intranasal challenge was conducted 4 weeks post-vaccination (PV) using 106 EID50/0.1 ml of the different virulent HPAI-H5N1 viruses representing clades 2.2.1, 2.2.1.1, 2.2.1.2, 2.3.4.4b-H5N8, and the vNDV. Results: The vaccine induced HI antibody titers of >6log2 against both H5N1 and NDV viruses at 2 weeks PV. Clinical protection against all HPAI H5N1 viruses and vNDV was 100%, except for HPAI H5N1 clade-2.2.1 and HPAI H5N8 clade- 2.3.4.4b viruses that showed 93.3% protection. Challenged SPF chickens showed significant decreases in the virus shedding titers up to <3log10 compared to challenge control chickens. No virus shedding was detected 6 "days post-challenge" in all vaccinated challenged groups. Conclusion: Our results indicate that the trivalent H5ND vaccine provides significant clinical protection against different clades of the HPAI viruses including the newly emerging H5N8 HPAI virus. Availability of such potent multivalent oil-emulsion vaccine offers an effective tool against HPAI control in endemic countries and promises simpler vaccination programs.

scholarly journals CAvant® WO-60 as an Effective Immunological Adjuvant for Avian Influenza and Newcastle Disease Vaccine

2021 ◽  
Vol 8 ◽  
Author(s):  
Eun-Seo Lee ◽  
Young-Jung Shim ◽  
W. A. Gayan Chathuranga ◽  
Young-Hoon Ahn ◽  
In-Joong Yoon ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
Antibody Responses ◽  
Economic Losses ◽  
Cell Mediated Immunity ◽  
Inactivated Vaccine ◽  
Aquatic Bird ◽  
Oil Emulsion ◽  
Water In Oil Emulsion ◽  
Newcastle Disease Vaccine

Despite the immunogenicity of vaccines currently used in poultry, several pathogens, including avian influenza virus (AIV) and Newcastle disease virus (NDV), cause enormous economic losses to the global poultry industry. The efficacy of vaccines can be improved by the introduction of effective adjuvants. This study evaluated a novel water-in-oil emulsion adjuvant, CAvant® WO-60, which effectively enhanced both the immunogenicity of conserved influenza antigen sM2HA2 and inactivated whole H9N2 antigen (iH9N2). CAvant® WO-60 induced both humoral and cell-mediated immunity in mice and provided 100% protection from challenge with 10 LD50 of A/Aquatic bird/Korea/W81/2005 (H5N2) and A/Chicken/Korea/116/2004 (H9N2) AIV. Importantly, immunization of chickens with iH9N2 plus inactivated NDV LaSota (iNDV) bivalent inactivated vaccine emulsified in CAvant® WO-60 induced seroprotective levels of antigen-specific antibody responses. Taken together, these results suggested that CAvant® WO-60 is a promising adjuvant for poultry vaccines.

Comparison of flagellin and an oil-emulsion adjuvant in inactivated Newcastle disease vaccine in stimulation of immunogenic parameters

2021 ◽  
Vol 75 ◽  
pp. 101622
Author(s):  
Maryam Barkhordari ◽  
Masoumeh Bagheri ◽  
Saeed Irian ◽  
Mohammad-Hosein Khani ◽  
Mohammad Majid Ebrahimi ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Oil Emulsion ◽  
Newcastle Disease Vaccine ◽  
Stimulation Of
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