Detection of Mycoplasma gallisepticum by Direct Immunofluorescence Using a Species-Specific Monoclonal Antibody

1986 ◽  
Vol 30 (1) ◽  
pp. 204 ◽  
Author(s):  
J. W. Morse ◽  
J. T. Boothby ◽  
R. Yamamoto
Keyword(s):  
Monoclonal Antibody ◽  
Mycoplasma Gallisepticum ◽  
Direct Immunofluorescence ◽  
Specific Monoclonal Antibody ◽  
Species Specific

scholarly journals Chlamydia pneumoniae Major Outer Membrane Protein Is a Surface-Exposed Antigen That Elicits Antibodies Primarily Directed against Conformation-Dependent Determinants

2001 ◽  
Vol 69 (5) ◽  
pp. 3082-3091 ◽  
Author(s):  
Katerina Wolf ◽  
Elizabeth Fischer ◽  
David Mead ◽  
Guangming Zhong ◽  
Roseanna Peeling ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Chlamydia Pneumoniae ◽  
Major Outer Membrane Protein ◽  
Mass Spectrometry Analysis ◽  
Antigenic Determinants ◽  
Specific Monoclonal Antibody ◽  
Species Specific

ABSTRACT The major outer membrane protein (MOMP) of Chlamydia trachomatis serovariants is known to be an immunodominant surface antigen. Moreover, it is known that the C. trachomatis MOMP elicits antibodies that recognize both linear and conformational antigenic determinants. In contrast, it has been reported that the MOMP of Chlamydia pneumoniae is not surface exposed and is immunorecessive. We hypothesized that the discrepancies betweenC. trachomatis and C. pneumoniae MOMP exposure on intact chlamydiae and immunogenic properties might be because the focus of the host's immune response is directed to conformational epitopes of the C. pneumoniae MOMP. We therefore conducted studies aimed at defining the surface exposure of MOMP and the conformational dominance of MOMP antibodies. We present here a description of C. pneumoniaespecies-specific monoclonal antibody (MAb), GZD1E8, which recognizes a conformational epitope on the surface of C. pneumoniae. This MAb is potent in the neutralization ofC. pneumoniae infectivity in vitro. Another previously described C. pneumoniaespecies-specific monoclonal antibody, RR-402, displayed very similar characteristics. However, the antigenic determinant recognized by RR-402 has yet to be identified. We show by immunoprecipitation ofC. pneumoniae with GZD1E8 and RR-402 MAbs and by mass spectrometry analysis of immunoprecipitated proteins that both antibodies GZD1E8 and RR-402 recognize the MOMP of C. pneumoniae and that this protein is localized on the surface of the organism. We also show that human sera fromC. pneumoniae-positive donors consistently recognize the MOMP by immunoprecipitation, indicating that the MOMP ofC. pneumoniae is an immunogenic protein. These findings have potential implications for both C. pneumoniae vaccine and diagnostic assay development.

scholarly journals Tick-Borne Relapsing Fever in British Columbia, Canada: First Isolation of Borrelia hermsii

1998 ◽  
Vol 36 (12) ◽  
pp. 3505-3508 ◽  
Author(s):  
Satyendra N. Banerjee ◽  
Maya Banerjee ◽  
Keerthi Fernando ◽  
Willy Burgdorfer ◽  
Tom G. Schwan
Keyword(s):  
Monoclonal Antibody ◽  
British Columbia ◽  
Plasmid Dna ◽  
Geographic Area ◽  
Protein Profiles ◽  
Specific Monoclonal Antibody ◽  
Relapsing Fever ◽  
Borrelia Hermsii ◽  
First Case ◽  
Species Specific

The spirochete that causes tick-borne relapsing fever,Borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern British Columbia, Canada. The geographic area of exposure is a known focus of this disease dating back to 1930 when the first case was recognized in a human. Analyses of plasmid DNA, protein profiles, and reactivity with a species-specific monoclonal antibody identified the new isolates of spirochetes as B. hermsii, all of which were most similar to an isolate of this spirochete from northern California described previously. These are the first reported isolates of B. hermsii from Canada.

scholarly journals Diagnostic and Prognostic Potential of a Competitive Enzyme-Linked Immunosorbent Assay for Leishmaniasis in India

1999 ◽  
Vol 6 (4) ◽  
pp. 550-554 ◽  
Author(s):  
Mitali Chatterjee ◽  
Charles L. Jaffe ◽  
Shyam Sundar ◽  
Debasis Basu ◽  
Sandeep Sen ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Leishmania Donovani ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Percent Inhibition ◽  
Chemotherapeutic Response ◽  
Before And After ◽  
Direct Elisa ◽  
Species Specific

ABSTRACT A Leishmania donovani species-specific monoclonal antibody (monoclonal antibody D2) was evaluated for its diagnostic and prognostic potential by a competitive enzyme-linked immunosorbent assay (C-ELISA) in sera from Indian patients with visceral leishmaniasis (VL) and seven patients with post-kala-azar dermal leishmaniasis (PKDL). These results were compared with those obtained by microscopy with Giemsa-stained tissue smears and a direct enzyme-linked immunosorbent assay (direct ELISA) with crude parasite antigen. Of 121 patients with clinically diagnosed VL examined, 103 (85.1%) were positive and 11 (9.1%) were negative by all three methods. An additional 7 (5.8%) who were negative by microscopy were positive by both C-ELISA and direct ELISA. Seven PKDL patients were also examined and were found to be positive by all three methods. Analysis of the chemotherapeutic response to sodium antimony gluconate of these 110 serologically positive VL patients showed that 57 (51.8%) were drug responsive and 53 (48.2%) were drug resistant. The C-ELISA with sera from 20 longitudinally monitored VL patients before and after chemotherapy showed a significant decrease in percent inhibition of monoclonal antibody D2 in drug-responsive patients. However, in drug-unresponsive patients, the percent inhibition of D2 was unchanged or was slightly increased. Our results therefore indicate (i) the applicability of L. donovani species-specific monoclonal antibody D2 for sensitive and specific serodiagnosis by C-ELISA, (ii) that the C-ELISA is more sensitive than microscopy, especially for early diagnosis, (iii) that L. donovani is still the main causative agent of VL, irrespective of the chemotherapeutic response, and (iv) that the C-ELISA can be used to evaluate the success of drug treatment.

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