Development of a species‐specific monoclonal antibody for rapid detection and identification of foodborne pathogen Vibrio vulnificus

2021 ◽  
Author(s):  
Pradit Wangman ◽  
Thanai Surasilp ◽  
Chalinan Pengsuk ◽  
Paisarn Sithigorngul ◽  
Siwaporn Longyant
Keyword(s):  
Monoclonal Antibody ◽  
Rapid Detection ◽  
Vibrio Vulnificus ◽  
Foodborne Pathogen ◽  
Specific Monoclonal Antibody ◽  
Detection And Identification ◽  
Species Specific

scholarly journals Chlamydia pneumoniae Major Outer Membrane Protein Is a Surface-Exposed Antigen That Elicits Antibodies Primarily Directed against Conformation-Dependent Determinants

2001 ◽  
Vol 69 (5) ◽  
pp. 3082-3091 ◽  
Author(s):  
Katerina Wolf ◽  
Elizabeth Fischer ◽  
David Mead ◽  
Guangming Zhong ◽  
Roseanna Peeling ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Chlamydia Pneumoniae ◽  
Major Outer Membrane Protein ◽  
Mass Spectrometry Analysis ◽  
Antigenic Determinants ◽  
Specific Monoclonal Antibody ◽  
Species Specific

ABSTRACT The major outer membrane protein (MOMP) of Chlamydia trachomatis serovariants is known to be an immunodominant surface antigen. Moreover, it is known that the C. trachomatis MOMP elicits antibodies that recognize both linear and conformational antigenic determinants. In contrast, it has been reported that the MOMP of Chlamydia pneumoniae is not surface exposed and is immunorecessive. We hypothesized that the discrepancies betweenC. trachomatis and C. pneumoniae MOMP exposure on intact chlamydiae and immunogenic properties might be because the focus of the host's immune response is directed to conformational epitopes of the C. pneumoniae MOMP. We therefore conducted studies aimed at defining the surface exposure of MOMP and the conformational dominance of MOMP antibodies. We present here a description of C. pneumoniaespecies-specific monoclonal antibody (MAb), GZD1E8, which recognizes a conformational epitope on the surface of C. pneumoniae. This MAb is potent in the neutralization ofC. pneumoniae infectivity in vitro. Another previously described C. pneumoniaespecies-specific monoclonal antibody, RR-402, displayed very similar characteristics. However, the antigenic determinant recognized by RR-402 has yet to be identified. We show by immunoprecipitation ofC. pneumoniae with GZD1E8 and RR-402 MAbs and by mass spectrometry analysis of immunoprecipitated proteins that both antibodies GZD1E8 and RR-402 recognize the MOMP of C. pneumoniae and that this protein is localized on the surface of the organism. We also show that human sera fromC. pneumoniae-positive donors consistently recognize the MOMP by immunoprecipitation, indicating that the MOMP ofC. pneumoniae is an immunogenic protein. These findings have potential implications for both C. pneumoniae vaccine and diagnostic assay development.

Rapid detection and identification of Streptococcus ratti by a species-specific PCR method

Anaerobe ◽  
2012 ◽  
Vol 18 (1) ◽  
pp. 44-47
Author(s):  
Jumpei Nishio ◽  
Makoto Taniguchi ◽  
Juichiro Higashi ◽  
Masakazu Takahashi ◽  
Takuma Ando ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Detection And Identification ◽  
Pcr Method ◽  
Species Specific ◽  
Streptococcus Ratti

scholarly journals Tick-Borne Relapsing Fever in British Columbia, Canada: First Isolation of Borrelia hermsii

1998 ◽  
Vol 36 (12) ◽  
pp. 3505-3508 ◽  
Author(s):  
Satyendra N. Banerjee ◽  
Maya Banerjee ◽  
Keerthi Fernando ◽  
Willy Burgdorfer ◽  
Tom G. Schwan
Keyword(s):  
Monoclonal Antibody ◽  
British Columbia ◽  
Plasmid Dna ◽  
Geographic Area ◽  
Protein Profiles ◽  
Specific Monoclonal Antibody ◽  
Relapsing Fever ◽  
Borrelia Hermsii ◽  
First Case ◽  
Species Specific

The spirochete that causes tick-borne relapsing fever,Borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern British Columbia, Canada. The geographic area of exposure is a known focus of this disease dating back to 1930 when the first case was recognized in a human. Analyses of plasmid DNA, protein profiles, and reactivity with a species-specific monoclonal antibody identified the new isolates of spirochetes as B. hermsii, all of which were most similar to an isolate of this spirochete from northern California described previously. These are the first reported isolates of B. hermsii from Canada.

Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation

2003 ◽  
Vol 81 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Marina Papadelli ◽  
Eugenia Manolopoulou ◽  
George Kalantzopoulos ◽  
Effie Tsakalidou
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Detection And Identification ◽  
Dna Hybridisation ◽  
Species Specific
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