Failure of Two Serotype II Infectious Bursal Disease Viruses to Affect the Humoral Immune Response of Turkeys

1984 ◽  
Vol 28 (1) ◽  
pp. 100 ◽  
Author(s):  
D. J. Jackwood ◽  
Y. M. Saif ◽  
P. D. Moorhead ◽  
G. Bishop
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease ◽  
Humoral Immune ◽  
Infectious Bursal Disease Viruses ◽  
Bursal Disease

scholarly journals Adjuvanticity of sliver nanoparticles with Infectious bursal disease virus VP2 protein and its effect on humoral immune response in chickens

2021 ◽  
Author(s):  
Sudhakar P. Awandkar ◽  
Prabhakar A Tembhurne ◽  
Nitin V Kurkure ◽  
Sandeep P Chaudhari ◽  
Sachin W Bonde ◽  
...  
Keyword(s):  
Immune Response ◽  
Silver Nanoparticles ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Gene Segment ◽  
Infectious Bursal Disease ◽  
Vp2 Protein ◽  
Humoral Immune ◽  
Bursal Disease

Abstract The present study was conducted to decipher the effect of silver nanoparticles conjugated marker recombinant VP2 immunogen of Infectious bursal disease virus on humoral immune response in chickens. The hypervariable VP2 gene segment of field Infectious bursal disease virus, consisting of major and minor hydrophilic loops, was amplified using reverse transcription-polymerase chain reaction. The gene segment of size 664 bp was cloned into pGEM-T Easy plasmid followed by subcloning into pET32a plasmid vector. Truncated recombinant VP2 protein (rVP2, 19 kDa) was expressed in a prokaryotic expression system using Escherichia coli BL32DE3 cells. The rVP2 protein showed reactivity with specific anti VP2 chicken antibodies. The results of Western blot revealed its utility in serological diagnosis. The recombinant antigen was tested for immunogenic potential by vaccinating the chickens with and without silver nanoparticles. The rVP2 protein blended with adjuvant grade montanide oil showed a highly significant rise in serum IgY titers. The titers induced by rVP2 protein mixed with montanide oil were non-significant when compared with titers induced by the conventional vaccines. The IgY response was highly significant in chickens vaccinated with rVP2 protein blended with montanide oil and silver nanoparticles than in chickens vaccinated with conventional vaccines or rVP2 protein. The results represent Infectious bursal disease virus rVP2 protein as a promising candidate for the DIVA vaccine and sero-diagnostic tool. For the first time, the current study elucidated the adjuvanticity effect of silver nanoparticles on avian Infectious bursal disease virus rVP2 vaccine potency.

scholarly journals Comparative Efficacy and Humoral Responses of an Inactivated Infectious Bursal Disease Virus Vaccine Prepared from a Local Isolate with that of a Commercial Live Vaccine in Layer Birds

1970 ◽  
Vol 2 (1) ◽  
pp. 37-38
Author(s):  
MIA Begum ◽  
MA Islam ◽  
ATM Mahbub-E-Elahi ◽  
M Rahman ◽  
MER Bhuiyan
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Live Vaccine ◽  
Infectious Bursal Disease ◽  
Inactivated Vaccine ◽  
Humoral Immune ◽  
Local Isolate ◽  
Bursal Disease

The humoral immune response and efficacy of an inactivated adjuvanted infectious bursal disease virus (IBDV) vaccine prepared with a virulent local isolate was compared with a live commercial IBDV vaccine (Nobilis D78®, Intervet) in 20 layer birds during the period from October to November 2002. These day-old experimental birds were divided into four groups, A, B, C and D, each consisting of 5 birds. Each bird of groups A, B and C was immunized with live IBDV vaccine (Nobilis D78®, Intervet), live + inactivated vaccine, and inactivated IBDV vaccine, respectively at day 7, day 21 and day 28, whereas birds of group D served as unvaccinated controls. The sera of chickens vaccinated with either combined (live + inactivated) or only inactivated IBDV vaccine showed clear band of precipitation with agar gel immunodiffusion test (AGIDT) and higher antibody titre with ELISA. The protection test revealed that the experimentally prepared inactivated IBDV vaccine gave 100% protection against 80% protection in layer birds immunized with live commercial vaccine. Key words: Efficacy; humoral immune response; IBD: inactivated vaccine; live vaccine; layer birds doi: 10.3329/bjvm.v2i1.1932 Bangl. J. Vet. Med. (2004). 2 (1) : 37-38

Field efficacy of different vaccines against infectious bursal disease in broiler flocks

2011 ◽  
Vol 59 (3) ◽  
pp. 385-398 ◽  
Author(s):  
Olga Zorman Rojs ◽  
Uroš Krapež ◽  
Brigita Slavec ◽  
Rahela Juršič-Cizerl ◽  
Tea Poljanec
Keyword(s):  
Immune Response ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Risk Area ◽  
Vector Vaccine ◽  
Humoral Immune ◽  
Production Parameters ◽  
Bursal Disease ◽  
Antibody Titres ◽  
High Level

A field study was performed to determine the efficacy of three commercially available vaccines against infectious bursal disease (IBD) in commercial broilers raised in a high IBD virus (IBDV) risk area. Live attenuated intermediate and intermediate plus vaccines were used in four flocks. Birds were vaccinated orally at the estimated vaccination time. Three broiler flocks were vaccinated subcutaneously with a turkey herpesvirus (HVT)-IBD vector vaccine at one day old. Evaluation of the efficacy of different vaccines was focused on humoral immune response, bursa/body weight (B/Bw) ratio, molecular detection of IBDV in ileocaecal tonsils and bursa of Fabricius, and production parameters. The serological results showed that although the uptake of all three vaccine strains was confirmed in the lymphoid organs, no significant antibody response to vaccination was detected in flocks vaccinated with intermediate and intermediate plus vaccines. A significant increase in antibody titres detected in flocks vaccinated with the vector vaccine indicated its ability to induce an immune response in birds with a high level of maternally derived antibodies. Observations obtained in this field trial did not confirm the expected reduction of the B/Bw ratio in flocks vaccinated with less attenuated vaccines. No significant differences were observed between birds vaccinated with the vector vaccine and those immunised with the intermediate plus vaccine. Very virulent IBDV was confirmed in the flock vaccinated with the intermediate vaccine. The infection induced reduced B/Bw and moderate mortality but did not affect the production parameters. Field infection was not detected in broilers vaccinated with the intermediate plus vaccine and the vector vaccine.

APPLICATION OF SHIFTING ASSAY OF STANDARDIZED INDIRECT AGGLUTINATION (SSIA) FOR DETECTION OF ANTIGENS OF NEWCASTLE DISEASE AND INFECTIOUS BURSAL DISEASE VIRUSES IN CHICKEN FAECES

2013 ◽  
Vol 83 (5) ◽  
Author(s):  
Phạm Hồng Sơn ◽  
Phạm Hồng Kỳ ◽  
Nguyễn Thị Lan Hương ◽  
Phạm Thị Hồng Hà
Keyword(s):  
Newcastle Disease ◽  
Winter Season ◽  
Summer Season ◽  
Infectious Bursal Disease ◽  
Infection Rates ◽  
Faecal Samples ◽  
Infectious Bursal Disease Viruses ◽  
Bursal Disease ◽  
Long Time ◽  
Higher Sensitivity

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi  accounted for the highest percentage of 25% of the infected chickens and Huong Thuy  the lowest  of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied.

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