Field efficacy of different vaccines against infectious bursal disease in broiler flocks

2011 ◽  
Vol 59 (3) ◽  
pp. 385-398 ◽  
Author(s):  
Olga Zorman Rojs ◽  
Uroš Krapež ◽  
Brigita Slavec ◽  
Rahela Juršič-Cizerl ◽  
Tea Poljanec
Keyword(s):  
Immune Response ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Risk Area ◽  
Vector Vaccine ◽  
Humoral Immune ◽  
Production Parameters ◽  
Bursal Disease ◽  
Antibody Titres ◽  
High Level

A field study was performed to determine the efficacy of three commercially available vaccines against infectious bursal disease (IBD) in commercial broilers raised in a high IBD virus (IBDV) risk area. Live attenuated intermediate and intermediate plus vaccines were used in four flocks. Birds were vaccinated orally at the estimated vaccination time. Three broiler flocks were vaccinated subcutaneously with a turkey herpesvirus (HVT)-IBD vector vaccine at one day old. Evaluation of the efficacy of different vaccines was focused on humoral immune response, bursa/body weight (B/Bw) ratio, molecular detection of IBDV in ileocaecal tonsils and bursa of Fabricius, and production parameters. The serological results showed that although the uptake of all three vaccine strains was confirmed in the lymphoid organs, no significant antibody response to vaccination was detected in flocks vaccinated with intermediate and intermediate plus vaccines. A significant increase in antibody titres detected in flocks vaccinated with the vector vaccine indicated its ability to induce an immune response in birds with a high level of maternally derived antibodies. Observations obtained in this field trial did not confirm the expected reduction of the B/Bw ratio in flocks vaccinated with less attenuated vaccines. No significant differences were observed between birds vaccinated with the vector vaccine and those immunised with the intermediate plus vaccine. Very virulent IBDV was confirmed in the flock vaccinated with the intermediate vaccine. The infection induced reduced B/Bw and moderate mortality but did not affect the production parameters. Field infection was not detected in broilers vaccinated with the intermediate plus vaccine and the vector vaccine.

scholarly journals Determination of the optimal time of vaccination against infectious bursal disease virus (Gumboro) in Algeria

2015 ◽  
Vol 82 (1) ◽  
Author(s):  
Omar Besseboua ◽  
Abdelhanine Ayad ◽  
Hama Benbarek
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Vaccination Schedule ◽  
Vaccine Virus ◽  
Infectious Bursal Disease ◽  
Optimal Time ◽  
Bursal Disease ◽  
Parent Stock ◽  
High Level

This study was conducted to determine the effect of maternally derived antibody (MDA) on live vaccine against infectious bursal disease. A total of 140 chicks selected from vaccinated parent stock were used in this investigation. In a preset vaccination schedule, blood samples were collected to check for the actual effect. It was noticed that on day 1 the chicks contained a high level (6400.54 ± 2993.67) of maternally derived antibody that gradually decreased below a positive level within 21 days (365.86 ± 634.46). It was found that a high level of MDA interferes with the vaccine virus, resulting in no immune response. For better immune response, it is suggested that the chickens should be vaccinated at day 21, as the uniformity of MDA is poor (coefficient of the variation [CV] > 30%), and boosted at day 28. Indeed, two vaccinations are necessary to achieve good protection against infectious bursal disease virus of the entire flock.

scholarly journals Adjuvanticity of sliver nanoparticles with Infectious bursal disease virus VP2 protein and its effect on humoral immune response in chickens

2021 ◽  
Author(s):  
Sudhakar P. Awandkar ◽  
Prabhakar A Tembhurne ◽  
Nitin V Kurkure ◽  
Sandeep P Chaudhari ◽  
Sachin W Bonde ◽  
...  
Keyword(s):  
Immune Response ◽  
Silver Nanoparticles ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Gene Segment ◽  
Infectious Bursal Disease ◽  
Vp2 Protein ◽  
Humoral Immune ◽  
Bursal Disease

Abstract The present study was conducted to decipher the effect of silver nanoparticles conjugated marker recombinant VP2 immunogen of Infectious bursal disease virus on humoral immune response in chickens. The hypervariable VP2 gene segment of field Infectious bursal disease virus, consisting of major and minor hydrophilic loops, was amplified using reverse transcription-polymerase chain reaction. The gene segment of size 664 bp was cloned into pGEM-T Easy plasmid followed by subcloning into pET32a plasmid vector. Truncated recombinant VP2 protein (rVP2, 19 kDa) was expressed in a prokaryotic expression system using Escherichia coli BL32DE3 cells. The rVP2 protein showed reactivity with specific anti VP2 chicken antibodies. The results of Western blot revealed its utility in serological diagnosis. The recombinant antigen was tested for immunogenic potential by vaccinating the chickens with and without silver nanoparticles. The rVP2 protein blended with adjuvant grade montanide oil showed a highly significant rise in serum IgY titers. The titers induced by rVP2 protein mixed with montanide oil were non-significant when compared with titers induced by the conventional vaccines. The IgY response was highly significant in chickens vaccinated with rVP2 protein blended with montanide oil and silver nanoparticles than in chickens vaccinated with conventional vaccines or rVP2 protein. The results represent Infectious bursal disease virus rVP2 protein as a promising candidate for the DIVA vaccine and sero-diagnostic tool. For the first time, the current study elucidated the adjuvanticity effect of silver nanoparticles on avian Infectious bursal disease virus rVP2 vaccine potency.

scholarly journals Comparative Efficacy and Humoral Responses of an Inactivated Infectious Bursal Disease Virus Vaccine Prepared from a Local Isolate with that of a Commercial Live Vaccine in Layer Birds

1970 ◽  
Vol 2 (1) ◽  
pp. 37-38
Author(s):  
MIA Begum ◽  
MA Islam ◽  
ATM Mahbub-E-Elahi ◽  
M Rahman ◽  
MER Bhuiyan
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Live Vaccine ◽  
Infectious Bursal Disease ◽  
Inactivated Vaccine ◽  
Humoral Immune ◽  
Local Isolate ◽  
Bursal Disease

The humoral immune response and efficacy of an inactivated adjuvanted infectious bursal disease virus (IBDV) vaccine prepared with a virulent local isolate was compared with a live commercial IBDV vaccine (Nobilis D78®, Intervet) in 20 layer birds during the period from October to November 2002. These day-old experimental birds were divided into four groups, A, B, C and D, each consisting of 5 birds. Each bird of groups A, B and C was immunized with live IBDV vaccine (Nobilis D78®, Intervet), live + inactivated vaccine, and inactivated IBDV vaccine, respectively at day 7, day 21 and day 28, whereas birds of group D served as unvaccinated controls. The sera of chickens vaccinated with either combined (live + inactivated) or only inactivated IBDV vaccine showed clear band of precipitation with agar gel immunodiffusion test (AGIDT) and higher antibody titre with ELISA. The protection test revealed that the experimentally prepared inactivated IBDV vaccine gave 100% protection against 80% protection in layer birds immunized with live commercial vaccine. Key words: Efficacy; humoral immune response; IBD: inactivated vaccine; live vaccine; layer birds doi: 10.3329/bjvm.v2i1.1932 Bangl. J. Vet. Med. (2004). 2 (1) : 37-38

scholarly journals Preclinical studies of immunogenity, protectivity, and safety of the combined vector vaccine for prevention of the middle east respiratory syndrome

Acta Naturae ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 114-123
Author(s):  
Inna V. Dolzhikova ◽  
D. M. Grousova ◽  
O. V. Zubkova ◽  
A. I. Tukhvatulin ◽  
A. V. Kovyrshina ◽  
...  
Keyword(s):  
Immune Response ◽  
Middle East ◽  
Mortality Rate ◽  
Receptor Gene ◽  
Human Adenovirus ◽  
T Cell Response ◽  
Middle East Respiratory Syndrome ◽  
Vector Vaccine ◽  
Humoral Immune ◽  
Cellular Immune

The Middle East Respiratory Syndrome (MERS) is an acute inflammatory disease of the respiratory system caused by the MERS-CoV coronavirus. The mortality rate for MERS is about 34.5%. Due to its high mortality rate, the lack of therapeutic and prophylactic agents, and the continuing threat of the spread of MERS beyond its current confines, developing a vaccine is a pressing task, because vaccination would help limit the spread of MERS and reduce its death toll. We have developed a combined vector vaccine for the prevention of MERS based on recombinant human adenovirus serotypes 26 and 5. Studies of its immunogenicity have shown that vaccination of animals (mice and primates) induces a robust humoral immune response that lasts for at least six months. Studies of the cellular immune response in mice after vaccination showed the emergence of a specific CD4+ and CD8+ T cell response. A study of the vaccine protectivity conducted in a model of transgenic mice carrying the human DPP4 receptor gene showed that our vaccination protected 100% of the animals from the lethal infection caused by the MERS-CoV virus (MERS-CoV EMC/2012, 100LD50 per mouse). Studies of the safety and tolerability of the developed vaccine in rodents, rabbits, and primates showed a good safety profile and tolerance in animals; they revealed no contraindications for clinical testing.

scholarly journals Adding hepatoprotective plants to beverage water: an alternative to conventional drugs for broiler production

2020 ◽  
Vol 14 (6) ◽  
pp. 1928-1940
Author(s):  
Dieudonné Pascal Chuisseu Djamen ◽  
Roland Nankam Chimi ◽  
Arouna Njayou Ngapagna ◽  
Leonard Tedong ◽  
François-Marie Kanmangne ◽  
...  
Keyword(s):  
Immune Response ◽  
Growth Parameters ◽  
Blood Parameters ◽  
Average Weight ◽  
Broiler Chicks ◽  
Humoral Immune ◽  
Growth Performances ◽  
Bursal Disease ◽  
Consumption Index ◽  
Kinetics Of

The present work was to study the effects of Desmoduin adscendens, Khaya grandifoliola, Xylopia phloiodora extracts on growth parameters and selected blood parameters of broilers chickens. A total of 252 broiler chicks were randomly distributed into 4 groups. Chickens of control batch received commercial hepatoprotective (Hepaturyl 1 g/l) and the experimental groups received a formulation based on 3 extracts hepatoprotective plants at a concentration of 200, 100 and 50 mg/kg body weight. The mortality rate of control and experimental groups was 6.3% and 4.7% respectively. Average weight of batches at day 48 was, 2.6 for the control, 2.7 for the batch 2, 2.6 batch 3 and 2.5 kg batch 4 with an average consumption index ranging from 1.6 for the control and 1.7 for the experimental groups. Liver function in broilers was not altered (The values of alanine aminotransferase and aspartate aminotransferase were 5-25 IU/l and 50-350 IU/l respectively). Cholesterolemia, proteinemia and the triglyceridemia increased with the age of the animals (0.6- 4.1 mmol / l; 21-83 g / l; 0.3- 3.8 g / l respectively). The kinetics of the humoral immune response against infectious bursal disease was not influenced. This work has shown that the use of the formulation as hepatoprotective in chick drinking water shows results similar to those of commercial hepatoprotectors.Keywords: Broilers, hepatoprotective plants, hepatic functioning, growth performances, immune response

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