Selection for Resistance to Marek's Disease in Conjunction with Other Economic Traits in Chickens

1972 ◽  
Vol 16 (1) ◽  
pp. 2 ◽  
Author(s):  
G. W. Friars ◽  
J. R. Chambers ◽  
A. Kennedy ◽  
A. D. Smith
Keyword(s):  
Marek's Disease ◽  
Marek’S Disease ◽  
Economic Traits ◽  
Selection For

Selection for Resistance to Marek's Disease and Its Expected Effects on Other Important Traits in White Leghorn Strain Crosses

1972 ◽  
Vol 16 (1) ◽  
pp. 11 ◽  
Author(s):  
C. M. von Krosigk ◽  
C. F. McClary ◽  
E. Vielitz ◽  
D. V. Zander
Keyword(s):  
Marek's Disease ◽  
Marek’S Disease ◽  
White Leghorn ◽  
Selection For

scholarly journals Influence of Selection for Egg Production and Marek's Disease Resistance on the Incidence of Endogenous Viral Genes in White Leghorns

1989 ◽  
Vol 68 (9) ◽  
pp. 1161-1167 ◽  
Author(s):  
U. KUHNLEIN ◽  
M. SABOUR ◽  
J.S. GAVORA ◽  
R.W. FAIRFULL ◽  
D.E. BERNON
Keyword(s):  
Disease Resistance ◽  
Egg Production ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Viral Genes ◽  
White Leghorns ◽  
Selection For

scholarly journals GENETIC RESISTANCE AND SUSCEPTIBILITY TO MAREK’S DISEASE

1970 ◽  
Vol 50 (1) ◽  
pp. 69-81 ◽  
Author(s):  
J. R. MORRIS ◽  
A. E. FERGUSON ◽  
F. N. JEROME
Keyword(s):  
Genetic Resistance ◽  
Small Population ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Base Population ◽  
Population Selection ◽  
Two Generations ◽  
Selection For ◽  
Successive Generations ◽  
Single Comb

Three thousand three hundred and thirty-five sire-pedigreed chicks from two commercial lines of Single Comb White Leghorns were inoculated with the Marek’s disease (MD) agent at one day of age and tested over a seven-week period for MD infection. Significant differences were observed between sexes and between lines with respect to MD mortality and total MD incidence. The females were more susceptible. Sire families in both lines showed heterogeneity in their ability to resist MD infection.A second experiment involving selection for resistance and for susceptibility to the disease was conducted on a population of University of Guelph Columbian Plymouth Rocks. Fully pedigreed chicks from this stock were inoculated and reared for 11 weeks while their full-sibs were reared separately to produce subsequent generations of selected families. Two successive generations of selection resulted in the formation of the resistant (R) and susceptible (S) lines. A random sample of the base population was propagated as a control population. Selection for MD resistance resulted in relatively greater progress than selection for MD susceptibility. After two generations of selection there was 12.5%, 54.1% and 61.9% MD mortality and 58.3%, 89.1% and 96.2% total MD incidence in the R, control and S lines, respectively. The more resistant the strain, the longer was the latent period before death. The variability between sexes and between half-sibs declined as MD mortality and total MD incidence approached high and low extremes. The results of the selection imposed showed progress despite the small population under test.

Selection for Increased Nitric Oxide Production Does Not Increase Resistance to Marek's Disease in a Primary Broiler Breeder Line

2009 ◽  
Vol 4 (3) ◽  
pp. e2-e2
Author(s):  
Celina Buscaglia ◽  
Priscilla H. O'Connell ◽  
Keith W. Jarosinski ◽  
Igal Pevzner ◽  
Karel A. Schat
Keyword(s):  
Nitric Oxide ◽  
Marek's Disease ◽  
Nitric Oxide Production ◽  
Marek’S Disease ◽  
Broiler Breeder ◽  
Oxide Production ◽  
Selection For

Selection for Increased Nitric Oxide Production Does Not Increase Resistance to Marek's Disease in a Primary Broiler Breeder Line

2009 ◽  
Vol 53 (3) ◽  
pp. 336-340 ◽  
Author(s):  
Celina Buscaglia ◽  
Priscilla H. O'Connell ◽  
Keith W. Jarosinski ◽  
Igal Pevzner ◽  
Karel A. Schat
Keyword(s):  
Nitric Oxide ◽  
Marek's Disease ◽  
Nitric Oxide Production ◽  
Marek’S Disease ◽  
Broiler Breeder ◽  
Oxide Production ◽  
Selection For

Electron Microscopy of a Herpesvirus Isolated from Marek's Disease in Duck and Chicken Embryo Fibroblast Cultures

1968 ◽  
Vol 26 ◽  
pp. 222-223 ◽  
Author(s):  
Keyvan Nazerian
Keyword(s):  
Inclusion Bodies ◽  
Chicken Embryo Fibroblast ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Duck Embryo Fibroblast ◽  
Multinucleated Cells ◽  
Viral Particles ◽  
Infected Cells ◽  
And Control ◽  
Do So

A herpes-like virus has been isolated from duck embryo fibroblast (DEF) cultures inoculated with blood from Marek's disease (MD) infected birds. Cultures which contained this virus produced MD in susceptible chickens while virus negative cultures and control cultures failed to do so. This and other circumstantial evidence including similarities in properties of the virus and the MD agent implicate this virus in the etiology of MD.Histochemical studies demonstrated the presence of DNA-staining intranuclear inclusion bodies in polykarocytes in infected cultures. Distinct nucleo-plasmic aggregates were also seen in sections of similar multinucleated cells examined with the electron microscope. These aggregates are probably the same as the inclusion bodies seen with the light microscope. Naked viral particles were observed in the nucleus of infected cells within or on the edges of the nucleoplasmic aggregates. These particles measured 95-100mμ, in diameter and rarely escaped into the cytoplasm or nuclear vesicles by budding through the nuclear membrane (Fig. 1). The enveloped particles (Fig. 2) formed in this manner measured 150-170mμ in diameter and always had a densely stained nucleoid. The virus in supernatant fluids consisted of naked capsids with 162 hollow, cylindrical capsomeres (Fig. 3). Enveloped particles were not seen in such preparations.

Isolation and identification of Marek’s disease virus (MDV) from feather follicle epithelium and internal organs of diseased chickens in Dakahlia Governorate, Egypt

2019 ◽  
Vol 20 (2) ◽  
pp. 6-11
Author(s):  
Aly El-Kenawy ◽  
Mohamed El-Tholoth ◽  
Emad A
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Field Samples ◽  
Feather Follicle ◽  
Positive Results

In the present study, a total of 16 samples including feather follicle epithelium, ovary, spleen and kidney (4 samples for each organ) were collected from diseased chicken flocks suspected to be infected with Marek’s disease virus (MDV) at Dakahlia Governorate, Egypt during the period from October 2016 to October 2017. Each sample was pooled randomly from three to five birds (90 to 360 days old). The isolation of the suspected virus from the collected samples was carried out via chorioallantoic membranes (CAMs) of 12 days old embryonated chicken eggs (ECEs). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard MDV. MDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). Molecular identification of virus was carried out by conventional polymerase chain reaction (PCR) and real- time PCR in four selected samples. The results revealed that 14 samples (87.5%) including 4 (100%) samples from feather follicle epithelium, ovary and kidney and 2 (50%) samples from spleen, showed positive results in virus isolation after 3rd passage. The positive results percentage by AGPT for field samples were 50% (8 out of 16 samples), while after the 3rd passage in ECEs were 37.5% (6 out of 16 samples) and the positive results percentage by IFAT for field samples were 62.5% (10 out of 16 samples), while after the 3rd passage in ECEs were 81.25 % (13 out of 16 samples). Viral nucleic acid was detected in all selected samples by conventional and real- time PCR. The results indicate that feather follicle epithelium is the best organ for MDV detection. IFAT is superior over AGPT in virus detection. Conventional and real - time PCR could be efficiently used for molecular detection of the virus.

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