Studies of Laryngotracheitis Virus in Avian Tissue Cultures V. Growth Characteristics of the Virus in Chicken Kidney and Chicken Embryo Kidney Tissue Cultures

1971 ◽  
Vol 15 (4) ◽  
pp. 730
Author(s):  
C. R. Rossi ◽  
A. M. Watrach
Keyword(s):  
Chicken Embryo ◽  
Kidney Tissue ◽  
Growth Characteristics ◽  
Tissue Cultures ◽  
Chicken Kidney ◽  
Laryngotracheitis Virus

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Melatonin receptors in the chicken kidney are up-regulated by pinealectomy and linked to adenylate cyclase

1996 ◽  
Vol 135 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Y Song ◽  
CS Pang ◽  
EA Ayre ◽  
GM Brown ◽  
SF Pang
Keyword(s):  
Hong Kong ◽  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Kidney Tissue ◽  
Melatonin Receptors ◽  
Melatonin Level ◽  
A Value ◽  
Biological Responses ◽  
Chicken Kidney ◽  
Camp Levels

Song Y, Pang CS, Ayre EA, Brown GM, Pang SF. Melatonin receptors in the chicken kidney are upregulated by pinealectomy and linked to adenylate cyclase. Eur J Endocrinol 1996;135:128–33. ISSN 0804–4643 The effect of pinealectomy on the characteristics of melatonin receptors in the chicken kidney was studied. One-day-old chicks were operated and kept under a 12 h/12 h light/dark photoperiod. Six weeks after operation, the animals were sacrificed at mid-light and mid-dark. Serum melatonin was determined by radioimmunoassay and kidney melatonin receptors were studied by radioreceptor assay using the melatonin agonist 2-[125I]iodomelatonin as the radioligand. Pinealectomy significantly reduced the mid-dark serum melatonin level and abolished the diurnal rhythm of 2-[125I]iodomelatonin binding in the kidney. The density of 2-[125I]Iodomelatonin binding sites in the kidney at mid-dark was increased significantly to a value comparable to the mid-light density after pineal ablation. Our results suggest that melatonin receptors in the chicken kidney are directly regulated by melatonin in the circulation. The coupling of kidney melatonin receptors to adenylate cyclase was investigated. The basal and forskolin-stimulated cAMP production in chicken kidney explants was studied following melatonin or melatonin plus pertussis toxin treatment. Levels of cAMP in chicken kidney explants were extracted and determined by radioimmunoassay. Melatonin had no effect on basal cAMP levels. However, melatonin significantly inhibited the forskolin-stimulated cAMP accumulation at a concentration of 10 pmol/l. Inhibitory effects of melatonin on the forskolin-stimulated cAMP increase in the chicken kidney were totally blocked by preincubating the kidney tissue with 1.0 μg/ml pertussis toxin. Our results suggest that kidney melatonin receptors may modulate the adenylate cyclase leading to biological responses in the renal system. SF Pang, Department of Physiology, Faculty of Medicine, University of Hong Kong, Hong Kong

COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: II. INVESTIGATION OF VARIOUS ENZYME SYSTEMS IN HOMOGENATES OF KIDNEY TISSUE CULTURES OF NORMAL RHESUS MONKEYS

1956 ◽  
Vol 34 (3) ◽  
pp. 600-618 ◽  
Author(s):  
E. Kovacs
Keyword(s):  
Acid Metabolism ◽  
Kidney Tissue ◽  
Nucleic Acid Metabolism ◽  
Enzyme Assays ◽  
Tissue Cultures ◽  
Functional Tests ◽  
Alkaline Phosphatases ◽  
Enzyme Systems ◽  
Tissue Cultivation ◽  
Considerable Loss

Pools of normal tissue cultures were examined for enzymes associated with nucleic acid metabolism. Ribonucleases and desoxyribonucleases, 5-nucleotidases, simple nucleotidases, acid and alkaline phosphatases were studied, and certain others occasionally demonstrated. Characteristic behavior of these enzyme systems during the cultivation procedures, during growth, and during degeneration was described. Quantitative data indicate the presence of significant amounts of enzymes in the supernatant fluid. This accounts for the considerable loss in these specialized constituents during fluid changes. The bearing of these findings on the physiology and pathology of cultivated cells was discussed, as a working hypothesis, with special emphasis on poliomyelitis infection. The use of enzyme assays, as functional tests supplementing morphological methods in tissue cultivation, was recommended.

Susceptibility of Pig Kidney Tissue Cultures to Certain Viruses

1957 ◽  
Vol 96 (2) ◽  
pp. 322-323 ◽  
Author(s):  
L. F. Guerin ◽  
M. M. Guerin
Keyword(s):  
Kidney Tissue ◽  
Tissue Cultures ◽  
Pig Kidney
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