Studies of laryngotracheitis virus in avian tissue cultures

1971 ◽  
Vol 35 (2-3) ◽  
pp. 223-231 ◽  
Author(s):  
C. R. Rossi
1970 ◽  
Vol 19 (6) ◽  
pp. 932-936
Author(s):  
C. R. Rossi ◽  
A. M. Watrach

1969 ◽  
Vol 28 (2) ◽  
pp. 219-228 ◽  
Author(s):  
C. R. Rossi ◽  
H. A. Reynolds ◽  
A. M. Watrach

1970 ◽  
Vol 19 (6) ◽  
pp. 932-936 ◽  
Author(s):  
C. R. Rossi ◽  
A. M. Watrach

Author(s):  
A. M. Watrach

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.


Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).


Author(s):  
R. D. Sjolund ◽  
C. Y. Shih

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.


1993 ◽  
Vol 88 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Patrick von Aderkas ◽  
Parker Anderson

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