A Simple Technique for Preparation of Chicken-Embryo-Skin Cell Cultures

1982 ◽  
Vol 26 (1) ◽  
pp. 182 ◽  
Author(s):  
A. Silim ◽  
M. A. S. Y. El Azhary ◽  
R. S. Roy
Keyword(s):  
Cell Cultures ◽  
Chicken Embryo ◽  
Simple Technique ◽  
Skin Cell

scholarly journals Counter-transport in chick embryo fibroblasts. A significant factor in measurement of glucose entry

1982 ◽  
Vol 206 (2) ◽  
pp. 301-309 ◽  
Author(s):  
R J Gay ◽  
H Amos
Keyword(s):  
Chick Embryo ◽  
Glucose Transport ◽  
Cell Cultures ◽  
Chicken Embryo ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts ◽  
Stimulation Of

Enhanced rates of carrier-mediated 3-O-methyl-D-glucose (0.1 mM) transport were observed in primary cell cultures of chicken embryo fibroblasts deprived of glucose for 1 day. The addition of 5.5 mM-glucose, glucosamine or 2-deoxy-D-glucose for 15 min (37 degrees C) to glucose-starved cultures followed by washing and immediate measurement of 3-O-methyl-D-glucose transport resulted in an apparent further stimulation of transport. Transport stimulation increased with increasing concentrations of the added preincubation sugar and was observed at test concentrations ranging from 0.1 mM- to 10 mM-3-O-methyl-D-glucose. This enhancement occurred when the preloaded sugar was rapidly effluxing from cells and was eliminated by allowing cultures to incubate in buffer without sugar for 30 min (37 degrees C) after the removal of hexose and before measuring transport. A transient overshoot in the cumulative uptake of 3-O-methyl-D-glucose was observed in glucose-starved cultures that were pre-incubated in the presence of 55 mM-glucose or -glucosamine for 15 min (37 degrees C). These data suggest that counter-transport accounts for the apparent enhancement of glucose-transport capability observed in glucose-starved cells when they are briefly re-exposed to hexose.

Human skin cell cultures onto PLA50(PDLLA) bioresorbable polymers: Influence of chemical and morphological surface modifications

2004 ◽  
Vol 72A (2) ◽  
pp. 180-189 ◽  
Author(s):  
Xavier Garric ◽  
Jean-Pierre Molès ◽  
Henri Garreau ◽  
Jean-Jacques Guilhou ◽  
Michel Vert
Keyword(s):  
Human Skin ◽  
Cell Cultures ◽  
Surface Modifications ◽  
Bioresorbable Polymers ◽  
Skin Cell

Quantitative analysis of MC1R gene expression in human skin cell cultures

2006 ◽  
Vol 19 (1) ◽  
pp. 76-89 ◽  
Author(s):  
Donald W. Roberts ◽  
Richard A. Newton ◽  
Kimberley A. Beaumont ◽  
J. Helen Leonard ◽  
Richard A. Sturm
Keyword(s):  
Gene Expression ◽  
Quantitative Analysis ◽  
Human Skin ◽  
Cell Cultures ◽  
Mc1r Gene ◽  
Skin Cell

Image analysis to quantify histological and immunofluorescent staining ofex vivoskin and skin cell cultures

2010 ◽  
Vol 32 (2) ◽  
pp. 143-154 ◽  
Author(s):  
R. L. McMullen ◽  
E. Bauza ◽  
C. Gondran ◽  
G. Oberto ◽  
N. Domloge ◽  
...  
Keyword(s):  
Image Analysis ◽  
Cell Cultures ◽  
Immunofluorescent Staining ◽  
Skin Cell

Growth and differentiation of chicken embryo muscle cell cultures derived from fast- and slow-growing lines

1984 ◽  
Vol 26 (1-3) ◽  
pp. 121-126 ◽  
Author(s):  
Julie F. Ridpath ◽  
Ted W. Huiatt ◽  
Allen H. Trenkle ◽  
Richard M. Robson ◽  
Peter J. Bechtel
Keyword(s):  
Muscle Cell ◽  
Cell Cultures ◽  
Chicken Embryo ◽  
Slow Growing
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