A Tissue Culture-Modified Newcastle Disease Virus. III. The Immunity Induced by the Modified Virus and Crystal Violet Inactivated Vaccines in Laying Birds

1958 ◽  
Vol 2 (4) ◽  
pp. 466
Author(s):  
R. A. Bankowski ◽  
R. Corstvet ◽  
J. Fabricant
Keyword(s):  
Tissue Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Crystal Violet ◽  
Newcastle Disease ◽  
Inactivated Vaccines

scholarly journals Effect of interferon on the development of Trypanosoma cruzi in tissue culture "Vero" cells

1980 ◽  
Vol 75 (1-2) ◽  
pp. 157-160 ◽  
Author(s):  
R. R. Golgher ◽  
M. S. M. Bertelli ◽  
M. L. Petrillo-Peixoto ◽  
Z. Brener
Keyword(s):  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vero Cells ◽  
Cell Infection ◽  
Amniotic Cells

Results are presented on the effects of interferon on the intracellular stages of T. cruzi in tissue culture "Vero" cells. Interferon was obtained by infecting monolayers of human amniotic cells with inactivated Newcastle disease virus. Interferon has not affected the cell infection by T. cruzi culture infective stages and neither has it prevented the transformation of amastigote into trypomastigote stages.

scholarly journals Immunoinformatics analysis and antibody epitope comparison of Newcastle disease virus classical vaccines with a virus involved in the fifth NDV panzootic

2021 ◽  
Author(s):  
Seyed-Elias Tabatabaeizadeh
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Newcastle Disease ◽  
Neutralizing Antibodies ◽  
3D Structure ◽  
Live Vaccines ◽  
Field Virus ◽  
Inactivated Vaccines ◽  
Antibody Epitopes

Abstract Newcastle disease virus (NDV) has negatively affected the poultry industry worldwide. Given that the antigenic similarity of a vaccine strain to a field virus is effective in protection, an immunoinformatics study was performed to examine the similarity between antibody epitopes of classical vaccines and a sub-genotype VII.2 NDV (VII.2 NDV). Considering the role of fusion (F) and hemagglutinin-neuraminidase (HN) proteins in the induction of neutralizing antibodies, the 3D structure of HN and F proteins of the VII.2 NDV and nine vaccine strains were predicted, refined, and validated. Using these structures, linear and conformational antibody epitopes were mapped. Epitope analysis showed distinct results from the evolutionary distance and protein identity analysis and it was found that the range of difference in the number of identical epitopes in relation to F is wider than HN protein. LaSota and B1 vaccine strains showed the least epitope identity to the VII.2 NDV. The V4 and I-2 vaccine strains showed the highest epitope identity with the VII.2 NDV especially in F protein which is important in virus cell-to-cell transmission. In conclusion, excellence of the LaSota vaccine under field condition shows that protection is not just about epitope similarities and especially in the case of live vaccines, the vaccine-induced damage, replicative capacity and tropism of the vaccine strain are important. The prediction of this study may be useful for inactivated vaccines in which the amount of antigen is all that matters.

scholarly journals The efficacy of binary ethylenimine-inactivated vaccines of Gianyar-1/AK/2014 virulent strain in protecting chickens against Tabanan-1/ARP/2017 virulent Newcastle disease virus isolates

2019 ◽  
Vol 12 (6) ◽  
pp. 758-764 ◽  
Author(s):  
Anak Agung Ayu Mirah Adi ◽  
I Nyoman Mantik Astawa ◽  
I Gusti Agung Arta Putra
Keyword(s):  
Antibody Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Post Vaccination ◽  
Group Iii ◽  
Group I ◽  
Virulent Newcastle Disease Virus ◽  
Inactivated Vaccines ◽  
The Mean

Aim: This study aimed to prepare binary ethylenimine (BEI)-inactivated virulent Newcastle disease virus (NDV) vaccine and to examine their ability to induce a protective antibody response in commercial chickens. Materials and Methods: A virulent NDV field isolate Gianyar-1/AK/2014 was propagated in chicken-embryonated eggs and was then inactivated with BEI at a concentration of 4 mM. Three groups of chickens with low-level (2 log2 hemagglutination inhibition [HI] units) maternally derived antibodies against NDV were then immunized with the BEI-inactivated vaccine. A commercial live vaccine (LaSota strain) was used as positive control, and phosphate-buffered saline (PBS) was used as negative control. A challenge experiment with a virulent NDV of Tabanan-1/ARP/2017 was performed at 3 weeks post-vaccination. Results: At 2 weeks post-immunization, the mean titers of antibodies against NDV in serum samples of chickens immunized with 0.2 mL of BEI-inactivated NDV (Group I), with live commercial NDV vaccine (Group II) and with PBS (Group III) were 3±0.94 log2 HI units, 4.9±0.99 log2 HI unit, and 0.0±0.0 HI units, respectively. At week 3 post-immunization, the mean titers of the antibodies for the three groups were 5±1.09 log2 HI units, 6.9±0.32 log2 HI units, and 0.00 HI units, respectively. The antibody titer induced by inactivated NDV Gianyar-1/AK/2014 isolates examined at 2 and 3 weeks post-vaccination was still at a significantly (p<0.01) lower level as compared to those induced by commercial life vaccine. However, the challenge test with virulent NDV of Tabanan 1/ARP/2017 isolates showed that all immunized chickens (Group I and II) survived without exhibiting any clinical sign post-challenge with the protection rates of 100%, whereas all chickens injected with PBS (Group III) died with clinical signs of ND. Conclusion: This finding shows that the BEI-inactivated vaccines prepared using virulent NDV of Gianyar-1/AK/2014 strain was able to induce protective antibody response in chickens but still at a lower level than those induce by commercial live NDV vaccine.

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