A new in vitro method (END) for detection and measurement of hog cholera virus and its antibody by means of effect of HC virus on newcastle disease virus in swine tissue culture

1964 ◽  
Vol 14 (2) ◽  
pp. 215-226 ◽  
Author(s):  
Takehiko Shimizu ◽  
Tetsuo Kumagai ◽  
Sumio Ikeda ◽  
Minoru Matumoto
Keyword(s):  
Tissue Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
In Vitro Method ◽  
Hog Cholera ◽  
Hog Cholera Virus ◽  
Swine Tissue

Transcription and translation of Newcastle disease virus mRNA's in vitro.

1978 ◽  
Vol 28 (1) ◽  
pp. 324-336 ◽  
Author(s):  
P L Collins ◽  
L E Hightower ◽  
L A Ball
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease

scholarly journals Role of the chicken oligoadenylate synthase-like gene during in vitro Newcastle disease virus infection

2021 ◽  
Vol 100 (5) ◽  
pp. 101067
Author(s):  
Ana Paula Del Vesco ◽  
Hyun Jun Jang ◽  
Melissa S. Monson ◽  
Susan J. Lamont
Keyword(s):  
Virus Infection ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Newcastle Disease Virus Infection

scholarly journals Persistent Newcastle disease virus infection in bladder cancer cells is associated with putative pro-survival and anti-viral transcriptomic changes

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lee-Chin Chan ◽  
Jeevanathan Kalyanasundram ◽  
Sze-Wei Leong ◽  
Mas Jaffri Masarudin ◽  
Abhi Veerakumarasivam ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Newcastle Disease Virus ◽  
Cancer Cells ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Persistent Infection ◽  
Signalling Pathways ◽  
Bladder Cancer Cells ◽  
Transcriptomic Changes

Abstract Background Newcastle disease virus (NDV) is an oncolytic virus with excellent selectivity against cancer cells, both in vitro and in vivo. Unfortunately, prolonged in vitro NDV infection results in the development of persistent infection in the cancer cells which are then able to resist NDV-mediated oncolysis. However, the mechanism of persistency of infection remains poorly understood. Methods In this study, we established persistently NDV-infected EJ28 bladder cancer cells, designated as EJ28P. Global transcriptomic analysis was subsequently carried out by microarray analysis. Differentially expressed genes (DEGs) between EJ28 and EJ28P cells identified by the edgeR program were further analysed by Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) analyses. In addition, the microarray data were validated by RT-qPCR. Results Persistently NDV-infected EJ28 bladder cancer cells were successfully established and confirmed by flow cytometry. Microarray analysis identified a total of 368 genes as differentially expressed in EJ28P cells when compared to the non-infected EJ28 cells. GSEA revealed that the Wnt/β-catenin and KRAS signalling pathways were upregulated while the TGF-β signalling pathway was downregulated. Findings from this study suggest that the upregulation of genes that are associated with cell growth, pro-survival, and anti-apoptosis may explain the survivability of EJ28P cells and the development of persistent infection of NDV. Conclusions This study provides insights into the transcriptomic changes that occur and the specific signalling pathways that are potentially involved in the development and maintenance of NDV persistency of infection in bladder cancer cells. These findings warrant further investigation and is crucial towards the development of effective NDV oncolytic therapy against cancer.

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