scholarly journals THE EFFECT OF LITHIUM AND O-IODOSOBENZOIC ACID ON THE EARLY DEVELOPMENT OF THE SEA URCHIN EGG

1963 ◽  
Vol 124 (1) ◽  
pp. 55-64 ◽  
Author(s):  
BERNDT E. HAGSTRÖM
Keyword(s):  
Early Development ◽  
Sea Urchin ◽  
Sea Urchin Egg ◽  
Iodosobenzoic Acid

scholarly journals THE BEHAVIOR OF THE NUCLEIC ACIDS DURING THE EARLY DEVELOPMENT OF THE SEA URCHIN EGG (ARBACIA)

1948 ◽  
Vol 31 (5) ◽  
pp. 457-457
Author(s):  
Gerhard Schmidt ◽  
Liselotte Hecht ◽  
S. J. Thannhauser
Keyword(s):  
Nucleic Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
P Fractions ◽  
Desoxyribonucleic Acid ◽  
Sea Urchin Egg

The authors wish to correct an error in the paper "The behavior of the nucleic acids during the early development of the sea urchin egg (Arbacia)" (J. Gen. Physiol., 1947–48, 31, 203). Owing to an oversight, the figures for the amounts of various P fractions in a single Arbacia egg have been erroneously expressed in γ x 10–3 units (Tables I and II, page 205; the last two lines of page 206). The figures should have been expressed in γ x 10–5 units. Thus, the fertilized Arbacia egg contains an average of 20 γ x 10–5 ribonucleic acid P and 0.7 to 1 γ x 10–5 desoxyribonucleic acid P.

scholarly journals Effects of Dithiothreitol on Fertilization and Early Development in Sea Urchin

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3573
Author(s):  
Nunzia Limatola ◽  
Jong Tai Chun ◽  
Sawsen Cherraben ◽  
Jean-Louis Schmitt ◽  
Jean-Marie Lehn ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Early Development ◽  
Sea Urchin ◽  
Actin Filaments ◽  
Cortical Granule ◽  
Cortical Actin ◽  
Phenotypic Changes ◽  
Sea Urchin Egg ◽  
Cortical Granule Exocytosis

The vitelline layer (VL) of a sea urchin egg is an intricate meshwork of glycoproteins that intimately ensheathes the plasma membrane. During fertilization, the VL plays important roles. Firstly, the receptors for sperm reside on the VL. Secondly, following cortical granule exocytosis, the VL is elevated and transformed into the fertilization envelope (FE), owing to the assembly and crosslinking of the extruded materials. As these two crucial stages involve the VL, its alteration was expected to affect the fertilization process. In the present study, we addressed this question by mildly treating the eggs with a reducing agent, dithiothreitol (DTT). A brief pretreatment with DTT resulted in partial disruption of the VL, as judged by electron microscopy and by a novel fluorescent polyamine probe that selectively labelled the VL. The DTT-pretreated eggs did not elevate the FE but were mostly monospermic at fertilization. These eggs also manifested certain anomalies at fertilization: (i) compromised Ca2+ signaling, (ii) blocked translocation of cortical actin filaments, and (iii) impaired cleavage. Some of these phenotypic changes were reversed by restoring the DTT-exposed eggs in normal seawater prior to fertilization. Our findings suggest that the FE is not the decisive factor preventing polyspermy and that the integrity of the VL is nonetheless crucial to the egg’s fertilization response.

The DNA synthetic period during early development of the sea urchin egg

1964 ◽  
Vol 36 (1) ◽  
pp. 53-61 ◽  
Author(s):  
R.T. Hinegardner ◽  
B. Rao ◽  
D.E. Feldman
Keyword(s):  
Early Development ◽  
Sea Urchin ◽  
Sea Urchin Egg

scholarly journals THE BEHAVIOR OF THE NUCLEIC ACIDS DURING THE EARLY DEVELOPMENT OF THE SEA URCHIN EGG (ARBACIA)

1948 ◽  
Vol 31 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Gerhard Schmidt ◽  
Liselotte Hecht ◽  
S. J. Thannhauser
Keyword(s):  
Nucleic Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Desoxyribonucleic Acid ◽  
Sea Urchin Egg

1. The unfertilized Arbacia egg contains an average of 20γ x 10–3 ribonucleic acid and 0.7 to 1γ x 10–3 desoxyribonucleic acid. 2. During the first 24 hours of development, the amount of ribonucleic acid per embryo remains practically unchanged whereas that of desoxyribonucleic acid steadily increases. At the end of this period, the amount of desoxyribonucleic acid per embryo is 10 to 15 times larger than that of the unfertilized egg.

Sea-urchin Development in the Light of Enzymic and Mitochondrial Studies

Development ◽  
1953 ◽  
Vol 1 (3) ◽  
pp. 251-255
Author(s):  
Tryggve Gustafson
Keyword(s):  
Amino Acids ◽  
Sea Urchin ◽  
Opposite Effect ◽  
Sea Water ◽  
Lithium Ions ◽  
Chemical Agents ◽  
Sea Urchin Egg ◽  
Biochemical Studies ◽  
Iodosobenzoic Acid ◽  
Very High

The sea-urchin egg is characterized by a very high morphogenetic plasticity. Its trend in differentiation can in fact be controlled by means of various chemical agents. The development of the entoderm is thus favoured at the expense of that of the ectoderm by adding lithium ions to the sea-water (Herbst, 1892; Lindahl, 1936). Iodosobenzoic acid (Runnström & Kriszat, 1952) and thiocyanate (Herbst, 1892; Lindahl, 1936) have an opposite effect, i.e. they favour ectodermal development. The mechanism of segregation of the egg into the primary germ-layers might be elucidated if we knew more about the biochemical mode of action of these agents. A series of biochemical studies on lithium-treated and normal eggs and larva was therefore undertaken. The studies began on the level of amino acids and peptides and continued on the levels of enzymes and intracellular inclusions. The total changes in the amino-acid composition of the egg were found to be rather small (Gustafson & Hjelte, 1951).

Sign in / Sign up

Export Citation Format

Share Document